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The map label for this gene is leuA [H]

Identifier: 209550383

GI number: 209550383

Start: 2848253

End: 2849986

Strand: Reverse

Name: leuA [H]

Synonym: Rleg2_2804

Alternate gene names: 209550383

Gene position: 2849986-2848253 (Counterclockwise)

Preceding gene: 209550384

Following gene: 209550378

Centisome position: 62.8

GC content: 60.55

Gene sequence:

>1734_bases
ATGAACGCAAAGACGCAATTCTCCGAGGGAAAAACGGCCTCCGCAAAAGGCATGCCTGACGCCGCGGTGAAATACCGGGC
CTATCCGCAGGTAAACATTCCCGACCGCACCTGGCCGACCAAGACCATCACCAAGGCGCCGGTCTGGTGCTCGGTCGACC
TGCGCGACGGCAACCAGGCGCTGGTCGACCCGATGGGCCACGACCGCAAGGCGCGGATGTTCCAGCTGCTGCTGGAGATG
GGCTTCAAGGAGATCGAGATCGGTTTCCCCTCCGCTTCGCAGACCGATTTCGATTTCGCCCGCTGGTGCGTGGAAGAGGG
CAACGTGCCCGCCGACGTCTCCCTGCAGGTGCTGGTGCAATGCCGCCCGGAACTCATCACCCGCACCTTCGAAGCACTGG
AAGGCGCAAACCGGCCGATCGTGCATTTTTACAACTCGACCAGCGAGCTGCAGCGCCGCGTCGTCTTCGCCAAGGATGTG
CAGGGCATCAAGCAGATCGCCGTCGATGCCGCCAAGATGATTACCGACATGGCCGTCAAGGCAGGCGGTGGCTACCGTTT
CGAATATTCGCCCGAGAGCTTTACCGGCACGGAACTCGAAGTGGCGCTGGAAATCTGCAATGGCGTCATCGAGGTGGTCA
AGCCGACCGCCGATAACAAGCTGATCATCAACCTGCCGTCCACCGTCGAGATGGCGACGCCGAACGTCTATGCCGACCAG
ATCGAGTGGATGTGCCGCAATCTCGACAATCGCGAGAACCTGATCATCTCCCTGCACCCGCATAACGACCGCGGCACCGG
CATCGCCGCTGCCGAGCTGGCGCTGCTGGCGGGCGCCGACCGTGTCGAAGGCACGCTCTTCGGCAATGGCGAGCGCACCG
GCAATGTCGACATGGTGACGATGGCGCTGAACATGTTCACGCAAGGCGTCGATCCCGAGATTGACTGCTCGAACATCGAG
CGCATCAAGGAAGTGTTCGAATATTCGAACCAGATGGCGATTTCCGAGCGTCATCCTTACGTCGGCGAGCTGGTCTATAC
GGCCTTTTCAGGCTCGCATCAGGATGCGATCAACAAGGGCATGAAGGCGGCGCAGGTCGCCAACCATCCCGTCTGGGAAG
TCCCATACCTGCCGATCGATCCGCGCGATGTCGGCCGTTCCTATGAGGCGATCATCCGCATCAACTCGCAGTCCGGCAAG
GGCGGCATCGCCTATATTCTGCAACAGGATTACGGGCTGAACCTGCCGCGCAACCTGCAGGTGGAGTTCCGTGAGGATAT
CCAGCGCATTACCGACGTAGAGGGCAAGGAGCTTCCTTCGAAGCGGATCTATGACCGTTTCATCGAGCGCTATGTGACGC
AGCCGAGCGCGCGCCTCAAATTCGTTGACCATCACACTTATCCGGACACCGAGCATAAGGGCCAGCGGATCGTGGCGGCT
GAGATTACCGACAATGGCGAGATCAAGCGTATCGAAGGGCGCGGCAACGGCCCGATCGACGGCTTCATCAACGCGCTGTC
GCATTATCTCGGCATCGAGATGTCGGTCGAAGACTATTCCGAACATTCGCTGCAGCACGGTTCGAACGCGGCGGCGATAT
CCTATGTCGAAACTTCCTATCCCGGCGGCAAGCTCTTCGGCGCCGGTGTCAATACCAACATCGTCGCGGCATCGCTGGAA
GCGATCGTCTCGGCGGCCAACCGCGTGCTCGAGGTGAAGGCCGGCAAGGCCTGA

Upstream 100 bases:

>100_bases
AAATTGACCTAACGACCGACAAAGGTCCGCATTTGTTTATCGCCAAGCCTGAGATGATCGGCTAAGAGCGGGGCAAATGC
CGGGACGAGGAGACGATACG

Downstream 100 bases:

>100_bases
TTCCGAAACGATTCAGGCCGCGCTCGTCTTGGTACGACGCGGCCTGGCGATTAGGTTGACTTCGTTGCGTCTGCGCTGAG
CGCAGGCGAAAGCAATGCGT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MNAKTQFSEGKTASAKGMPDAAVKYRAYPQVNIPDRTWPTKTITKAPVWCSVDLRDGNQALVDPMGHDRKARMFQLLLEM
GFKEIEIGFPSASQTDFDFARWCVEEGNVPADVSLQVLVQCRPELITRTFEALEGANRPIVHFYNSTSELQRRVVFAKDV
QGIKQIAVDAAKMITDMAVKAGGGYRFEYSPESFTGTELEVALEICNGVIEVVKPTADNKLIINLPSTVEMATPNVYADQ
IEWMCRNLDNRENLIISLHPHNDRGTGIAAAELALLAGADRVEGTLFGNGERTGNVDMVTMALNMFTQGVDPEIDCSNIE
RIKEVFEYSNQMAISERHPYVGELVYTAFSGSHQDAINKGMKAAQVANHPVWEVPYLPIDPRDVGRSYEAIIRINSQSGK
GGIAYILQQDYGLNLPRNLQVEFREDIQRITDVEGKELPSKRIYDRFIERYVTQPSARLKFVDHHTYPDTEHKGQRIVAA
EITDNGEIKRIEGRGNGPIDGFINALSHYLGIEMSVEDYSEHSLQHGSNAAAISYVETSYPGGKLFGAGVNTNIVAASLE
AIVSAANRVLEVKAGKA

Sequences:

>Translated_577_residues
MNAKTQFSEGKTASAKGMPDAAVKYRAYPQVNIPDRTWPTKTITKAPVWCSVDLRDGNQALVDPMGHDRKARMFQLLLEM
GFKEIEIGFPSASQTDFDFARWCVEEGNVPADVSLQVLVQCRPELITRTFEALEGANRPIVHFYNSTSELQRRVVFAKDV
QGIKQIAVDAAKMITDMAVKAGGGYRFEYSPESFTGTELEVALEICNGVIEVVKPTADNKLIINLPSTVEMATPNVYADQ
IEWMCRNLDNRENLIISLHPHNDRGTGIAAAELALLAGADRVEGTLFGNGERTGNVDMVTMALNMFTQGVDPEIDCSNIE
RIKEVFEYSNQMAISERHPYVGELVYTAFSGSHQDAINKGMKAAQVANHPVWEVPYLPIDPRDVGRSYEAIIRINSQSGK
GGIAYILQQDYGLNLPRNLQVEFREDIQRITDVEGKELPSKRIYDRFIERYVTQPSARLKFVDHHTYPDTEHKGQRIVAA
EITDNGEIKRIEGRGNGPIDGFINALSHYLGIEMSVEDYSEHSLQHGSNAAAISYVETSYPGGKLFGAGVNTNIVAASLE
AIVSAANRVLEVKAGKA
>Mature_577_residues
MNAKTQFSEGKTASAKGMPDAAVKYRAYPQVNIPDRTWPTKTITKAPVWCSVDLRDGNQALVDPMGHDRKARMFQLLLEM
GFKEIEIGFPSASQTDFDFARWCVEEGNVPADVSLQVLVQCRPELITRTFEALEGANRPIVHFYNSTSELQRRVVFAKDV
QGIKQIAVDAAKMITDMAVKAGGGYRFEYSPESFTGTELEVALEICNGVIEVVKPTADNKLIINLPSTVEMATPNVYADQ
IEWMCRNLDNRENLIISLHPHNDRGTGIAAAELALLAGADRVEGTLFGNGERTGNVDMVTMALNMFTQGVDPEIDCSNIE
RIKEVFEYSNQMAISERHPYVGELVYTAFSGSHQDAINKGMKAAQVANHPVWEVPYLPIDPRDVGRSYEAIIRINSQSGK
GGIAYILQQDYGLNLPRNLQVEFREDIQRITDVEGKELPSKRIYDRFIERYVTQPSARLKFVDHHTYPDTEHKGQRIVAA
EITDNGEIKRIEGRGNGPIDGFINALSHYLGIEMSVEDYSEHSLQHGSNAAAISYVETSYPGGKLFGAGVNTNIVAASLE
AIVSAANRVLEVKAGKA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=530, Percent_Identity=24.5283018867925, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324682, Length=565, Percent_Identity=46.3716814159292, Blast_Score=517, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6324225, Length=579, Percent_Identity=45.5958549222798, Blast_Score=508, Evalue=1e-144,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63880; Mature: 63880

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKTQFSEGKTASAKGMPDAAVKYRAYPQVNIPDRTWPTKTITKAPVWCSVDLRDGNQA
CCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEE
LVDPMGHDRKARMFQLLLEMGFKEIEIGFPSASQTDFDFARWCVEEGNVPADVSLQVLVQ
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
CRPELITRTFEALEGANRPIVHFYNSTSELQRRVVFAKDVQGIKQIAVDAAKMITDMAVK
CCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGGGYRFEYSPESFTGTELEVALEICNGVIEVVKPTADNKLIINLPSTVEMATPNVYADQ
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHH
IEWMCRNLDNRENLIISLHPHNDRGTGIAAAELALLAGADRVEGTLFGNGERTGNVDMVT
HHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHH
MALNMFTQGVDPEIDCSNIERIKEVFEYSNQMAISERHPYVGELVYTAFSGSHQDAINKG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHEECCCCCCHHHHHHC
MKAAQVANHPVWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYILQQDYGLNLPRNLQ
CHHHHHCCCCCEECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCC
VEFREDIQRITDVEGKELPSKRIYDRFIERYVTQPSARLKFVDHHTYPDTEHKGQRIVAA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEE
EITDNGEIKRIEGRGNGPIDGFINALSHYLGIEMSVEDYSEHSLQHGSNAAAISYVETSY
EECCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCEEEEEEECCC
PGGKLFGAGVNTNIVAASLEAIVSAANRVLEVKAGKA
CCCEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCC
>Mature Secondary Structure
MNAKTQFSEGKTASAKGMPDAAVKYRAYPQVNIPDRTWPTKTITKAPVWCSVDLRDGNQA
CCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEE
LVDPMGHDRKARMFQLLLEMGFKEIEIGFPSASQTDFDFARWCVEEGNVPADVSLQVLVQ
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
CRPELITRTFEALEGANRPIVHFYNSTSELQRRVVFAKDVQGIKQIAVDAAKMITDMAVK
CCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AGGGYRFEYSPESFTGTELEVALEICNGVIEVVKPTADNKLIINLPSTVEMATPNVYADQ
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHH
IEWMCRNLDNRENLIISLHPHNDRGTGIAAAELALLAGADRVEGTLFGNGERTGNVDMVT
HHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHH
MALNMFTQGVDPEIDCSNIERIKEVFEYSNQMAISERHPYVGELVYTAFSGSHQDAINKG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHEECCCCCCHHHHHHC
MKAAQVANHPVWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYILQQDYGLNLPRNLQ
CHHHHHCCCCCEECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCC
VEFREDIQRITDVEGKELPSKRIYDRFIERYVTQPSARLKFVDHHTYPDTEHKGQRIVAA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEE
EITDNGEIKRIEGRGNGPIDGFINALSHYLGIEMSVEDYSEHSLQHGSNAAAISYVETSY
EECCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCEEEEEEECCC
PGGKLFGAGVNTNIVAASLEAIVSAANRVLEVKAGKA
CCCEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA