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The map label for this gene is yafC [C]

Identifier: 209550098

GI number: 209550098

Start: 2549048

End: 2549974

Strand: Reverse

Name: yafC [C]

Synonym: Rleg2_2515

Alternate gene names: 209550098

Gene position: 2549974-2549048 (Counterclockwise)

Preceding gene: 209550103

Following gene: 209550097

Centisome position: 56.19

GC content: 65.59

Gene sequence:

>927_bases
ATGGATATCGTTTCGGCATTGCGGACCTTCCAGCGCGTCGTGGAGACGGGCTCGTTTTCGGCGGCGGCACACGATCTCGA
CGTGACGCAGCCGGCGGTCTCGCGGCAGGTCGCAGCCCTTGAGGGGCATTTCAACACGCGCCTCCTGCACCGCACGACGA
GTGGCCTGTCGCTGACGGCGGAGGGGGAGCGCATGCTGCCGATGGCGCTCAGGATTCTCGAAGCGGTGGAGGAACTCGGC
GATGCCACTGGATCGGACGGCGTCATGGCTTCCGGGAAGGTCAGGCTCAGTGTTCCGGCACCGCTCGGCCTCTATCTCAG
CGAGCAGCTGGGCGATCTTCTTGCCGCCCACCCGAAACTCTCGGTGGAACTGCTCTTCAGGGAGCAGAGCTCCGATATGA
TAGAGGAGCGCCTCGACCTCGAAGTGCGCCTCGGCTCGGTCGCCGACAGCAGCCTGGTCTGCCGCCGGATCGGCTGGACG
ACGGCCTTCCTCGGCGCATCGCCCGCCTATCTCGCGCGTAAGGCGCCGCCGCGGACGCCGAAGGACATCAAGGACCATGA
ATGCCTGTGCTATAACAGGGCGGGCGATGCCGCGACATGGTCGTTCTCCAACGGCTCCGAAGACATTTCGGTTCGGATCT
CGCCGCGGCTGACCGCCTGCAGCGCCGTCGCCATTCACCGGGCAGCCCTTGCCGGCGCGGGCCTGGCCGTGCTTTCCCAT
ATCATCGCCGTGCCCGATATTATCGCCGGCCGGCTGATCCCCGTGATGGAGGATTTTCAGCCGAGCCGGCTGCCGATCAC
CGTCGTCTATCCCTCGCGGCGGAACATGCCGCTGCGCGTCAAGACGGTTCTGGATTTTCTGACCGATGCCATCGGGCGGG
ATCCGTCGATGTGCGCGAGCGGCATGGGCTGGCAACAGAACGGATGA

Upstream 100 bases:

>100_bases
TCCTTATGGATAAACGATATCCGTAATTGCTGGACTGTAAAGCCATTCCAGCCTAGTTTGCCTGCATAAAGCCTATGCGC
AAAAGTATGAGGGACAGGGG

Downstream 100 bases:

>100_bases
TTGTCTCACCGGGCGATTTGCGGTTTTCCGCCTTGCCTAACGCCTGCGAATCCTGCCAAGTCGTCCGATCATGCAATTTG
CGCCGCAACAAGACGAAGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MDIVSALRTFQRVVETGSFSAAAHDLDVTQPAVSRQVAALEGHFNTRLLHRTTSGLSLTAEGERMLPMALRILEAVEELG
DATGSDGVMASGKVRLSVPAPLGLYLSEQLGDLLAAHPKLSVELLFREQSSDMIEERLDLEVRLGSVADSSLVCRRIGWT
TAFLGASPAYLARKAPPRTPKDIKDHECLCYNRAGDAATWSFSNGSEDISVRISPRLTACSAVAIHRAALAGAGLAVLSH
IIAVPDIIAGRLIPVMEDFQPSRLPITVVYPSRRNMPLRVKTVLDFLTDAIGRDPSMCASGMGWQQNG

Sequences:

>Translated_308_residues
MDIVSALRTFQRVVETGSFSAAAHDLDVTQPAVSRQVAALEGHFNTRLLHRTTSGLSLTAEGERMLPMALRILEAVEELG
DATGSDGVMASGKVRLSVPAPLGLYLSEQLGDLLAAHPKLSVELLFREQSSDMIEERLDLEVRLGSVADSSLVCRRIGWT
TAFLGASPAYLARKAPPRTPKDIKDHECLCYNRAGDAATWSFSNGSEDISVRISPRLTACSAVAIHRAALAGAGLAVLSH
IIAVPDIIAGRLIPVMEDFQPSRLPITVVYPSRRNMPLRVKTVLDFLTDAIGRDPSMCASGMGWQQNG
>Mature_308_residues
MDIVSALRTFQRVVETGSFSAAAHDLDVTQPAVSRQVAALEGHFNTRLLHRTTSGLSLTAEGERMLPMALRILEAVEELG
DATGSDGVMASGKVRLSVPAPLGLYLSEQLGDLLAAHPKLSVELLFREQSSDMIEERLDLEVRLGSVADSSLVCRRIGWT
TAFLGASPAYLARKAPPRTPKDIKDHECLCYNRAGDAATWSFSNGSEDISVRISPRLTACSAVAIHRAALAGAGLAVLSH
IIAVPDIIAGRLIPVMEDFQPSRLPITVVYPSRRNMPLRVKTVLDFLTDAIGRDPSMCASGMGWQQNG

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=31.0810810810811, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=30.8219178082192, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=28.4246575342466, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=28.0701754385965, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=28.125, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=26.4604810996564, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=30.6049822064057, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789173, Length=268, Percent_Identity=27.2388059701493, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693105, Length=155, Percent_Identity=27.0967741935484, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790011, Length=289, Percent_Identity=23.8754325259516, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33155; Mature: 33155

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVSALRTFQRVVETGSFSAAAHDLDVTQPAVSRQVAALEGHFNTRLLHRTTSGLSLTA
CCHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEE
EGERMLPMALRILEAVEELGDATGSDGVMASGKVRLSVPAPLGLYLSEQLGDLLAAHPKL
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCC
SVELLFREQSSDMIEERLDLEVRLGSVADSSLVCRRIGWTTAFLGASPAYLARKAPPRTP
EEEEEEECCCHHHHHHHHCEEEEECCCCCHHHHHHHHCCHHHHHCCCHHHHHCCCCCCCC
KDIKDHECLCYNRAGDAATWSFSNGSEDISVRISPRLTACSAVAIHRAALAGAGLAVLSH
CCCCCCCEEEEECCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
IIAVPDIIAGRLIPVMEDFQPSRLPITVVYPSRRNMPLRVKTVLDFLTDAIGRDPSMCAS
HHHHHHHHHCCHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHC
GMGWQQNG
CCCCCCCC
>Mature Secondary Structure
MDIVSALRTFQRVVETGSFSAAAHDLDVTQPAVSRQVAALEGHFNTRLLHRTTSGLSLTA
CCHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEE
EGERMLPMALRILEAVEELGDATGSDGVMASGKVRLSVPAPLGLYLSEQLGDLLAAHPKL
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCC
SVELLFREQSSDMIEERLDLEVRLGSVADSSLVCRRIGWTTAFLGASPAYLARKAPPRTP
EEEEEEECCCHHHHHHHHCEEEEECCCCCHHHHHHHHCCHHHHHCCCHHHHHCCCCCCCC
KDIKDHECLCYNRAGDAATWSFSNGSEDISVRISPRLTACSAVAIHRAALAGAGLAVLSH
CCCCCCCEEEEECCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
IIAVPDIIAGRLIPVMEDFQPSRLPITVVYPSRRNMPLRVKTVLDFLTDAIGRDPSMCAS
HHHHHHHHHCCHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHC
GMGWQQNG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]