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The map label for this gene is pdhB [H]

Identifier: 209549203

GI number: 209549203

Start: 1631178

End: 1632563

Strand: Direct

Name: pdhB [H]

Synonym: Rleg2_1604

Alternate gene names: 209549203

Gene position: 1631178-1632563 (Clockwise)

Preceding gene: 209549202

Following gene: 209549204

Centisome position: 35.95

GC content: 61.4

Gene sequence:

>1386_bases
ATGCCTATCGATATCCTCATGCCCGCCCTCTCTCCGACCATGGAAGAAGGCACGCTGTCCAAATGGCTGAAGCAGGAAGG
TGACAAGGTCACTTCTGGCGACGTCATTGCCGAAATCGAAACCGACAAGGCGACGATGGAAGTCGAAGCCGTCGACGAAG
GCGTCATCGGCAAGCTGCTCGTCGATGCCGGCACCGAAGGCGTCAAGGTCAACACCAAGATCGCCGTGCTGCTGCAGGAT
GGCGAATCGGCCGCGGATATCTCCGCCGCCAAGCCGGCTGCTGCTGCCGCACCTCAGGCTGCCCAGGAAGAAAAGCCGAC
GAATAGCGGCTCGGCTTCCGCACCGCTTCCGGCCGAGCCGAAGGCCGTCGTGCCGAATGACCCGGAAATTCCGGCCGGCA
CCGAAATGGTGTCGATGACGGTGCGCGAAGCGCTCCGTGACGCCATGGCCGAGGAAATGCGCGCCAGCGAAGATGTCTTC
GTCATGGGCGAGGAAGTCGCCGAATATCAGGGCGCCTACAAGGTCACGCAAGGGTTGCTGCAGGAATTCGGCCCCCGCCG
CGTCATCGATACGCCGATCACCGAGCACGGCTTTGCCGGCGTCGGCGTCGGCGCCGCCATGGCCGGCCTTCGCCCGATCG
TCGAATTCATGACCTTCAACTTCGCCATGCAGGCGATCGACCACATCATCAACTCGGCTGCCAAGACGCTCTATATGTCC
GGCGGCCAGATGGGCGCTCCGATCGTCTTCCGCGGCCCGAACGGTGCAGCCGCCCGCGTCGGCGCCCAGCACAGCCAGGA
TTATGCCGCCTGGTACAGCGCCATCCCCGGCCTGAAGGTCGTCATGCCTTACACGGCAGCCGACGCAAAGGGCCTGTTGA
AGGCTGCGATCCGCGATCCGAACCCGGTCATCTTCCTGGAAAACGAAATTCTCTACGGTCAGCATTTCGATGTGCCGAAG
CTCGATAATTTCGTCCTGCCGATCGGCAAGGCCCGCATCCATCGTTCTGGCAAGGATGTCACCGTCGTCTCCTTCGGCAT
CGGCATGACCTATGCGACGAAGGCGGTTGCCGAACTCGAAAAGATCGGCATCGACGTCGAACTGATCGACCTTCGCACCA
TTCGCCCGATGGATCTCCCGACCGTGATCGAATCGGTGAAGAAGACCGGCCGCCTCGTCACTGTCGAGGAAGGTTATCCG
CAATCTTCAGTCGGCACCGAAATCGCCACCCGCGTCATGCAGCAGGCCTTCGACTATCTCGATGCGCCGATCCTGACGAT
CGCGGGCAAGGACGTGCCGATGCCCTACGCCGCCAATCTCGAAAAACTGGCGCTTCCGAACGTCGGCGAAGTCGTCGATG
CGGTGAAGGCTGTTTGCTATAAATAA

Upstream 100 bases:

>100_bases
CGACATCGTCGCCGACAGCGCCGACTTCGCCCAGGCCGATCCGGAGCCGGATGCATCCGCGCTCTACACCGACATTCTGC
TCTAATCGGGGAGGGAACCC

Downstream 100 bases:

>100_bases
GGGGAGGGTATCTCGATGCCGATCAATATCACGATGCCCGCCCTCTCTCCGACCATGGAAGAAGGCAATCTTTCCAAATG
GCTGGTCAAGGAAGGCGATA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPIDILMPALSPTMEEGTLSKWLKQEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLLVDAGTEGVKVNTKIAVLLQD
GESAADISAAKPAAAAAPQAAQEEKPTNSGSASAPLPAEPKAVVPNDPEIPAGTEMVSMTVREALRDAMAEEMRASEDVF
VMGEEVAEYQGAYKVTQGLLQEFGPRRVIDTPITEHGFAGVGVGAAMAGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMS
GGQMGAPIVFRGPNGAAARVGAQHSQDYAAWYSAIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQHFDVPK
LDNFVLPIGKARIHRSGKDVTVVSFGIGMTYATKAVAELEKIGIDVELIDLRTIRPMDLPTVIESVKKTGRLVTVEEGYP
QSSVGTEIATRVMQQAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVGEVVDAVKAVCYK

Sequences:

>Translated_461_residues
MPIDILMPALSPTMEEGTLSKWLKQEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLLVDAGTEGVKVNTKIAVLLQD
GESAADISAAKPAAAAAPQAAQEEKPTNSGSASAPLPAEPKAVVPNDPEIPAGTEMVSMTVREALRDAMAEEMRASEDVF
VMGEEVAEYQGAYKVTQGLLQEFGPRRVIDTPITEHGFAGVGVGAAMAGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMS
GGQMGAPIVFRGPNGAAARVGAQHSQDYAAWYSAIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQHFDVPK
LDNFVLPIGKARIHRSGKDVTVVSFGIGMTYATKAVAELEKIGIDVELIDLRTIRPMDLPTVIESVKKTGRLVTVEEGYP
QSSVGTEIATRVMQQAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVGEVVDAVKAVCYK
>Mature_460_residues
PIDILMPALSPTMEEGTLSKWLKQEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLLVDAGTEGVKVNTKIAVLLQDG
ESAADISAAKPAAAAAPQAAQEEKPTNSGSASAPLPAEPKAVVPNDPEIPAGTEMVSMTVREALRDAMAEEMRASEDVFV
MGEEVAEYQGAYKVTQGLLQEFGPRRVIDTPITEHGFAGVGVGAAMAGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMSG
GQMGAPIVFRGPNGAAARVGAQHSQDYAAWYSAIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQHFDVPKL
DNFVLPIGKARIHRSGKDVTVVSFGIGMTYATKAVAELEKIGIDVELIDLRTIRPMDLPTVIESVKKTGRLVTVEEGYPQ
SSVGTEIATRVMQQAFDYLDAPILTIAGKDVPMPYAANLEKLALPNVGEVVDAVKAVCYK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=57.0987654320988, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=53.3950617283951, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI4557353, Length=333, Percent_Identity=33.6336336336336, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI34101272, Length=333, Percent_Identity=33.6336336336336, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI203098753, Length=90, Percent_Identity=44.4444444444444, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=48.1481481481481, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI31711992, Length=146, Percent_Identity=37.6712328767123, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=57.6271186440678, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI225637461, Length=256, Percent_Identity=30.078125, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI225637459, Length=256, Percent_Identity=30.078125, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI225637463, Length=256, Percent_Identity=30.078125, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI133778974, Length=283, Percent_Identity=27.5618374558304, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI205277463, Length=302, Percent_Identity=24.5033112582781, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4507521, Length=302, Percent_Identity=24.5033112582781, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=61.3003095975232, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17506935, Length=334, Percent_Identity=40.4191616766467, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17560088, Length=140, Percent_Identity=39.2857142857143, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=58.1039755351682, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324258, Length=124, Percent_Identity=42.741935483871, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321632, Length=101, Percent_Identity=38.6138613861386, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21358145, Length=322, Percent_Identity=59.9378881987578, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24650940, Length=322, Percent_Identity=59.9378881987578, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=34.0557275541796, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=34.0557275541796, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=65.5555555555556, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=65.5555555555556, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI20129315, Length=83, Percent_Identity=46.9879518072289, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45551847, Length=241, Percent_Identity=29.045643153527, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550715, Length=241, Percent_Identity=29.045643153527, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24645119, Length=241, Percent_Identity=29.045643153527, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582497, Length=72, Percent_Identity=44.4444444444444, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49025; Mature: 48894

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDILMPALSPTMEEGTLSKWLKQEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLL
CCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHH
VDAGTEGVKVNTKIAVLLQDGESAADISAAKPAAAAAPQAAQEEKPTNSGSASAPLPAEP
HHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCHHCCCCHHHHCCCCCCCCCCCCCCCCC
KAVVPNDPEIPAGTEMVSMTVREALRDAMAEEMRASEDVFVMGEEVAEYQGAYKVTQGLL
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHH
QEFGPRRVIDTPITEHGFAGVGVGAAMAGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMS
HHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
GGQMGAPIVFRGPNGAAARVGAQHSQDYAAWYSAIPGLKVVMPYTAADAKGLLKAAIRDP
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCC
NPVIFLENEILYGQHFDVPKLDNFVLPIGKARIHRSGKDVTVVSFGIGMTYATKAVAELE
CCEEEEECCEEECCCCCCCCCCCEEECCCHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
KIGIDVELIDLRTIRPMDLPTVIESVKKTGRLVTVEEGYPQSSVGTEIATRVMQQAFDYL
HHCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DAPILTIAGKDVPMPYAANLEKLALPNVGEVVDAVKAVCYK
CCCEEEECCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIDILMPALSPTMEEGTLSKWLKQEGDKVTSGDVIAEIETDKATMEVEAVDEGVIGKLL
CCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHH
VDAGTEGVKVNTKIAVLLQDGESAADISAAKPAAAAAPQAAQEEKPTNSGSASAPLPAEP
HHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCHHCCCCHHHHCCCCCCCCCCCCCCCCC
KAVVPNDPEIPAGTEMVSMTVREALRDAMAEEMRASEDVFVMGEEVAEYQGAYKVTQGLL
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHH
QEFGPRRVIDTPITEHGFAGVGVGAAMAGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMS
HHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
GGQMGAPIVFRGPNGAAARVGAQHSQDYAAWYSAIPGLKVVMPYTAADAKGLLKAAIRDP
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCC
NPVIFLENEILYGQHFDVPKLDNFVLPIGKARIHRSGKDVTVVSFGIGMTYATKAVAELE
CCEEEEECCEEECCCCCCCCCCCEEECCCHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
KIGIDVELIDLRTIRPMDLPTVIESVKKTGRLVTVEEGYPQSSVGTEIATRVMQQAFDYL
HHCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DAPILTIAGKDVPMPYAANLEKLALPNVGEVVDAVKAVCYK
CCCEEEECCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]