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The map label for this gene is pdhC [H]

Identifier: 209549204

GI number: 209549204

Start: 1632579

End: 1633919

Strand: Direct

Name: pdhC [H]

Synonym: Rleg2_1605

Alternate gene names: 209549204

Gene position: 1632579-1633919 (Clockwise)

Preceding gene: 209549203

Following gene: 209549205

Centisome position: 35.98

GC content: 66.14

Gene sequence:

>1341_bases
ATGCCGATCAATATCACGATGCCCGCCCTCTCTCCGACCATGGAAGAAGGCAATCTTTCCAAATGGCTGGTCAAGGAAGG
CGATACGGTCAAGTCTGGCGACGTGATCGCCGAGATCGAGACCGACAAGGCGACGATGGAAGTCGAAGCCGTCGATGAAG
GCACAGTCGCCAAGCTCGTCGTTGCCGCCGGCACCGAAGGTGTCAAGGTCAACGCGCTGATCGCCGTTCTCGCCGCCGAT
GGCGAAGATGTCGCCGCTGCCGCAAGCGGTGCGGGCTCCGCCGCTCCGGCGAAGGCCGAGGCCGCACCCGCACCGAAGGC
CGAGGCTGCACCTGCTCCGGCAGCAGCGCCGGCGCCTGCCGCCGCACCTGCGGCGGTTTCATCAAACGGCAACCGCAGCT
TCTCTTCGCCGCTTGCTCGCAGGCTCGCCAAGGAAGCTGGTATCGATCTTTCGGCTGTCGCCGGCTCCGGACCGCACGGC
CGCGTCATCAAGAGCGACGTCGAAGCCGCCGTTGCCGGTGGTGGCGCCAAACCTGCCGCCGCGCCGGCCGCTGCCGCCCC
GCAGGCCGCCGCCGCTCCGGCTCCGGCCGCAGCGCCGAAGGGCGCTTCCGAGGACGCCGTGCTCAAGCTCTTCGAGCCGG
GCTCCTACGAGCTCGTGCCGCATGACGGCATGCGCAAGACGATTGCCCGGCGCCTGGTCGAATCCAAGCAGACGATCCCG
CATTTCTATGTCAGCGTCGATTGCGAACTCGATGCGCTGATGGCGCTGCGCGCCCAGCTGAACGACGCCGCCCCCCGCAA
GGACAATGCTCCGGCCTACAAGCTCTCGGTCAACGACATGGTCATCAAGGCCATGGCGCTGGCGCTGCGCGACGTGCCGG
ATGCCAACGTCTCATGGACCGACACCAACATGGTCAAGCACAAGCATGCCGATGTCGGCGTCGCCGTCTCGATCCCCGGC
GGCCTGATCACCCCGATCATCCGCAAGGCCGAGCAGAAGACGCTGTCCGTCATCTCCAACGAGATGCGCGATCTCGGCAA
GCGGGCCAAGGACCGGAAGCTGAAGCCCGAGGAATATCAGGGCGGCACCAGCTCGGTTTCGAACATGGGCATGATGGGCG
TCAAGAACTTCGCCGCCGTCGTCAACCCGCCGCATGCGACGATCCTCGCAGTCGGCGCCGGCGAACAGCGGGTCGTCGTC
AAGAACGGCGAAATGGCGATCGCCACCGTGATGTCGGTCACGCTGTCGACCGACCATCGCTGCGTCGACGGCGCGCTCGG
CGCCGAGCTGCTCCAGGCCTTCAAGGGCTACATCGAGAACCCGATGGGCATGCTTGTCTGA

Upstream 100 bases:

>100_bases
GCCCTACGCCGCCAATCTCGAAAAACTGGCGCTTCCGAACGTCGGCGAAGTCGTCGATGCGGTGAAGGCTGTTTGCTATA
AATAAGGGGAGGGTATCTCG

Downstream 100 bases:

>100_bases
TAGCTGGGCGGAGGCGGAAATGACCAAGACCGTTCTTTGCTATGGCGACTCGCTGACCTGGGGTTATGACGCCGACACGA
TCGGCCGTCATGAGTACAAA

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPINITMPALSPTMEEGNLSKWLVKEGDTVKSGDVIAEIETDKATMEVEAVDEGTVAKLVVAAGTEGVKVNALIAVLAAD
GEDVAAAASGAGSAAPAKAEAAPAPKAEAAPAPAAAPAPAAAPAAVSSNGNRSFSSPLARRLAKEAGIDLSAVAGSGPHG
RVIKSDVEAAVAGGGAKPAAAPAAAAPQAAAAPAPAAAPKGASEDAVLKLFEPGSYELVPHDGMRKTIARRLVESKQTIP
HFYVSVDCELDALMALRAQLNDAAPRKDNAPAYKLSVNDMVIKAMALALRDVPDANVSWTDTNMVKHKHADVGVAVSIPG
GLITPIIRKAEQKTLSVISNEMRDLGKRAKDRKLKPEEYQGGTSSVSNMGMMGVKNFAAVVNPPHATILAVGAGEQRVVV
KNGEMAIATVMSVTLSTDHRCVDGALGAELLQAFKGYIENPMGMLV

Sequences:

>Translated_446_residues
MPINITMPALSPTMEEGNLSKWLVKEGDTVKSGDVIAEIETDKATMEVEAVDEGTVAKLVVAAGTEGVKVNALIAVLAAD
GEDVAAAASGAGSAAPAKAEAAPAPKAEAAPAPAAAPAPAAAPAAVSSNGNRSFSSPLARRLAKEAGIDLSAVAGSGPHG
RVIKSDVEAAVAGGGAKPAAAPAAAAPQAAAAPAPAAAPKGASEDAVLKLFEPGSYELVPHDGMRKTIARRLVESKQTIP
HFYVSVDCELDALMALRAQLNDAAPRKDNAPAYKLSVNDMVIKAMALALRDVPDANVSWTDTNMVKHKHADVGVAVSIPG
GLITPIIRKAEQKTLSVISNEMRDLGKRAKDRKLKPEEYQGGTSSVSNMGMMGVKNFAAVVNPPHATILAVGAGEQRVVV
KNGEMAIATVMSVTLSTDHRCVDGALGAELLQAFKGYIENPMGMLV
>Mature_445_residues
PINITMPALSPTMEEGNLSKWLVKEGDTVKSGDVIAEIETDKATMEVEAVDEGTVAKLVVAAGTEGVKVNALIAVLAADG
EDVAAAASGAGSAAPAKAEAAPAPKAEAAPAPAAAPAPAAAPAAVSSNGNRSFSSPLARRLAKEAGIDLSAVAGSGPHGR
VIKSDVEAAVAGGGAKPAAAPAAAAPQAAAAPAPAAAPKGASEDAVLKLFEPGSYELVPHDGMRKTIARRLVESKQTIPH
FYVSVDCELDALMALRAQLNDAAPRKDNAPAYKLSVNDMVIKAMALALRDVPDANVSWTDTNMVKHKHADVGVAVSIPGG
LITPIIRKAEQKTLSVISNEMRDLGKRAKDRKLKPEEYQGGTSSVSNMGMMGVKNFAAVVNPPHATILAVGAGEQRVVVK
NGEMAIATVMSVTLSTDHRCVDGALGAELLQAFKGYIENPMGMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=452, Percent_Identity=40.4867256637168, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI203098816, Length=462, Percent_Identity=36.7965367965368, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI203098753, Length=462, Percent_Identity=36.7965367965368, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=27.3972602739726, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=42.5, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1786946, Length=444, Percent_Identity=29.0540540540541, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786305, Length=420, Percent_Identity=32.6190476190476, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=457, Percent_Identity=42.6695842450766, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17538894, Length=317, Percent_Identity=33.4384858044164, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=435, Percent_Identity=31.264367816092, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=452, Percent_Identity=29.646017699115, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=459, Percent_Identity=43.1372549019608, Blast_Score=308, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6320352, Length=449, Percent_Identity=28.5077951002227, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6321632, Length=176, Percent_Identity=32.9545454545455, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI20129315, Length=461, Percent_Identity=40.1301518438178, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24582497, Length=450, Percent_Identity=39.5555555555556, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI18859875, Length=445, Percent_Identity=28.7640449438202, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=30.3964757709251, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45539; Mature: 45407

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINITMPALSPTMEEGNLSKWLVKEGDTVKSGDVIAEIETDKATMEVEAVDEGTVAKLV
CCEEEECCCCCCCCCCCCCHHHHCCCCCCEECCCEEEEEECCCEEEEEEECCCCCEEEEE
VAAGTEGVKVNALIAVLAADGEDVAAAASGAGSAAPAKAEAAPAPKAEAAPAPAAAPAPA
EECCCCCEEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAPAAVSSNGNRSFSSPLARRLAKEAGIDLSAVAGSGPHGRVIKSDVEAAVAGGGAKPAA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCEEHHHHHHHHCCCCCCCCC
APAAAAPQAAAAPAPAAAPKGASEDAVLKLFEPGSYELVPHDGMRKTIARRLVESKQTIP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCC
HFYVSVDCELDALMALRAQLNDAAPRKDNAPAYKLSVNDMVIKAMALALRDVPDANVSWT
EEEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEC
DTNMVKHKHADVGVAVSIPGGLITPIIRKAEQKTLSVISNEMRDLGKRAKDRKLKPEEYQ
CCHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
GGTSSVSNMGMMGVKNFAAVVNPPHATILAVGAGEQRVVVKNGEMAIATVMSVTLSTDHR
CCCHHHHHCCCHHHHHHHHEECCCCEEEEEECCCCCEEEEECCCEEEEEEEEHHCCCCCC
CVDGALGAELLQAFKGYIENPMGMLV
HHHHHHHHHHHHHHHHHHCCCCHHCC
>Mature Secondary Structure 
PINITMPALSPTMEEGNLSKWLVKEGDTVKSGDVIAEIETDKATMEVEAVDEGTVAKLV
CEEEECCCCCCCCCCCCCHHHHCCCCCCEECCCEEEEEECCCEEEEEEECCCCCEEEEE
VAAGTEGVKVNALIAVLAADGEDVAAAASGAGSAAPAKAEAAPAPKAEAAPAPAAAPAPA
EECCCCCEEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAPAAVSSNGNRSFSSPLARRLAKEAGIDLSAVAGSGPHGRVIKSDVEAAVAGGGAKPAA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCEEHHHHHHHHCCCCCCCCC
APAAAAPQAAAAPAPAAAPKGASEDAVLKLFEPGSYELVPHDGMRKTIARRLVESKQTIP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCC
HFYVSVDCELDALMALRAQLNDAAPRKDNAPAYKLSVNDMVIKAMALALRDVPDANVSWT
EEEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEC
DTNMVKHKHADVGVAVSIPGGLITPIIRKAEQKTLSVISNEMRDLGKRAKDRKLKPEEYQ
CCHHHHEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
GGTSSVSNMGMMGVKNFAAVVNPPHATILAVGAGEQRVVVKNGEMAIATVMSVTLSTDHR
CCCHHHHHCCCHHHHHHHHEECCCCEEEEEECCCCCEEEEECCCEEEEEEEEHHCCCCCC
CVDGALGAELLQAFKGYIENPMGMLV
HHHHHHHHHHHHHHHHHHCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]