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The map label for this gene is phaZ1 [H]

Identifier: 209549066

GI number: 209549066

Start: 1489981

End: 1491072

Strand: Reverse

Name: phaZ1 [H]

Synonym: Rleg2_1463

Alternate gene names: 209549066

Gene position: 1491072-1489981 (Counterclockwise)

Preceding gene: 209549068

Following gene: 209549064

Centisome position: 32.86

GC content: 60.9

Gene sequence:

>1092_bases
ATGAGATCTATGTCCGACACGCTTGCGCGCCTTGCTGCGTTGCGAGGAACGCTTCGCCCCCGCCCCACGCAGGAAAATCC
CGATCTGATCGCCCTGGACGGATTTGGTGCAAACCCCGGGGCTCTGGGAGGTTACATCTACCTGCCGTCGAAGCGTGCAA
AGAACATGGCCCTGGTCACCGTCCTGCACGGCTGCACCCAGACAGCATCAGGCTATGACATCGGGTCGGGGTGGTCTGGG
TTGGCAGAGGATTATGGTTTCGCGCTGCTGTTTCCCGAGCAACGAAGGGCGAACAACCCCAACCTCTGCTTCAACTGGTT
TAACCCCGAGGATATACGCCGGGATCACGGCGAGGTCTTTTCCATTCGCCAGATGATCGCAAAACTCGTTGCGGAGCACA
GCATCGACAGCAGGCGCGTGTTCGTAACCGGGCTTTCGGCCGGCGGCGCCATGACCGCCGCCATGCTTGCCACCTATCCG
GAGATTTTTGCCGGAGGGGCCATCATTGCAGGCCTGCCCTATGCCAGCGCCGCGACCATTCCCGAGGCGTTCGACCGAAT
GCGCGGTCATGGCGGCCCGACCGCCAAGGAACTGCAACTGCGGTTGAGAAACGCCTCAGGCCACAAAGGCCCGTGGCCGA
CTTTGTCGCTCTGGCAGGGAAGCGCAGACAGAACCGTCGTGCCATCCAATGCGAATGCCATCGTGGAGCAATGGAGAAAT
ATCCACGGCATCGACGCATCGCCGCCGCGCGTGGAAAATATCGGACGCCATCAGCGAAGGGTCTGGACCGATGCTCATGG
GGTGGAGGTGATCGAACTCTATACGATTGCCGGAATGGATCACGGCACGCCGCTTGACGTCGGCATCGGCTACGGGGCGA
GCGGCCCCTTCATGCTTGACGTCGGAATTTCCTCGACCGTCGAAATCGCCAGGTCCTGGGGCCTGGTTCCCTCGTTTGAG
AAGCGGCACAATGCAAAACCCGCTGCTGCGCAACCGGAAAACGTCGCAGGTTTATCTGCCATAGGGGATGATCGCGGCGG
CATTCAGAAGGTCATCGAAGACGCCCTTCGGTCTGCCGGGCTGCTGCGGTAA

Upstream 100 bases:

>100_bases
GCCTGCTCAGAAAATATTGTGCAGCGCAGCAACCTTCCGCCGACTGAGGCGTTTACGCAGGCATCACCGCCATCCCGGCT
GCTGATTGGCGGAGACTTTC

Downstream 100 bases:

>100_bases
AGCGACAGAACAGGAGCAAACTGGCTGCCATGCCATTCGCCGGGGGACCGTCCAGATCCAGCGAGATTGCCGCTATCGGT
TCTGAGACTTGGGCAGCGGC

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MRSMSDTLARLAALRGTLRPRPTQENPDLIALDGFGANPGALGGYIYLPSKRAKNMALVTVLHGCTQTASGYDIGSGWSG
LAEDYGFALLFPEQRRANNPNLCFNWFNPEDIRRDHGEVFSIRQMIAKLVAEHSIDSRRVFVTGLSAGGAMTAAMLATYP
EIFAGGAIIAGLPYASAATIPEAFDRMRGHGGPTAKELQLRLRNASGHKGPWPTLSLWQGSADRTVVPSNANAIVEQWRN
IHGIDASPPRVENIGRHQRRVWTDAHGVEVIELYTIAGMDHGTPLDVGIGYGASGPFMLDVGISSTVEIARSWGLVPSFE
KRHNAKPAAAQPENVAGLSAIGDDRGGIQKVIEDALRSAGLLR

Sequences:

>Translated_363_residues
MRSMSDTLARLAALRGTLRPRPTQENPDLIALDGFGANPGALGGYIYLPSKRAKNMALVTVLHGCTQTASGYDIGSGWSG
LAEDYGFALLFPEQRRANNPNLCFNWFNPEDIRRDHGEVFSIRQMIAKLVAEHSIDSRRVFVTGLSAGGAMTAAMLATYP
EIFAGGAIIAGLPYASAATIPEAFDRMRGHGGPTAKELQLRLRNASGHKGPWPTLSLWQGSADRTVVPSNANAIVEQWRN
IHGIDASPPRVENIGRHQRRVWTDAHGVEVIELYTIAGMDHGTPLDVGIGYGASGPFMLDVGISSTVEIARSWGLVPSFE
KRHNAKPAAAQPENVAGLSAIGDDRGGIQKVIEDALRSAGLLR
>Mature_363_residues
MRSMSDTLARLAALRGTLRPRPTQENPDLIALDGFGANPGALGGYIYLPSKRAKNMALVTVLHGCTQTASGYDIGSGWSG
LAEDYGFALLFPEQRRANNPNLCFNWFNPEDIRRDHGEVFSIRQMIAKLVAEHSIDSRRVFVTGLSAGGAMTAAMLATYP
EIFAGGAIIAGLPYASAATIPEAFDRMRGHGGPTAKELQLRLRNASGHKGPWPTLSLWQGSADRTVVPSNANAIVEQWRN
IHGIDASPPRVENIGRHQRRVWTDAHGVEVIELYTIAGMDHGTPLDVGIGYGASGPFMLDVGISSTVEIARSWGLVPSFE
KRHNAKPAAAQPENVAGLSAIGDDRGGIQKVIEDALRSAGLLR

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38982; Mature: 38982

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSMSDTLARLAALRGTLRPRPTQENPDLIALDGFGANPGALGGYIYLPSKRAKNMALVT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEH
VLHGCTQTASGYDIGSGWSGLAEDYGFALLFPEQRRANNPNLCFNWFNPEDIRRDHGEVF
HHHHHCCCCCCCCCCCCCCHHHHHCCEEEEECHHHCCCCCCEEEECCCHHHHHHHHHHHH
SIRQMIAKLVAEHSIDSRRVFVTGLSAGGAMTAAMLATYPEIFAGGAIIAGLPYASAATI
HHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHH
PEAFDRMRGHGGPTAKELQLRLRNASGHKGPWPTLSLWQGSADRTVVPSNANAIVEQWRN
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEECCCCHHHHHHHHHH
IHGIDASPPRVENIGRHQRRVWTDAHGVEVIELYTIAGMDHGTPLDVGIGYGASGPFMLD
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCEEEE
VGISSTVEIARSWGLVPSFEKRHNAKPAAAQPENVAGLSAIGDDRGGIQKVIEDALRSAG
ECCCHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCC
LLR
CCC
>Mature Secondary Structure
MRSMSDTLARLAALRGTLRPRPTQENPDLIALDGFGANPGALGGYIYLPSKRAKNMALVT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEH
VLHGCTQTASGYDIGSGWSGLAEDYGFALLFPEQRRANNPNLCFNWFNPEDIRRDHGEVF
HHHHHCCCCCCCCCCCCCCHHHHHCCEEEEECHHHCCCCCCEEEECCCHHHHHHHHHHHH
SIRQMIAKLVAEHSIDSRRVFVTGLSAGGAMTAAMLATYPEIFAGGAIIAGLPYASAATI
HHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHH
PEAFDRMRGHGGPTAKELQLRLRNASGHKGPWPTLSLWQGSADRTVVPSNANAIVEQWRN
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEECCCCHHHHHHHHHH
IHGIDASPPRVENIGRHQRRVWTDAHGVEVIELYTIAGMDHGTPLDVGIGYGASGPFMLD
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCEEEE
VGISSTVEIARSWGLVPSFEKRHNAKPAAAQPENVAGLSAIGDDRGGIQKVIEDALRSAG
ECCCHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCC
LLR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]