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The map label for this gene is yiaO [H]

Identifier: 209549060

GI number: 209549060

Start: 1484020

End: 1485018

Strand: Direct

Name: yiaO [H]

Synonym: Rleg2_1457

Alternate gene names: 209549060

Gene position: 1484020-1485018 (Clockwise)

Preceding gene: 209549059

Following gene: 209549061

Centisome position: 32.7

GC content: 61.36

Gene sequence:

>999_bases
ATGAACAATCTCAACCGACGTAATTTCCTGAGGACCGCCGCTCTAACCGGTACGGCGCTTGCGGCGCCAGGCTTTGTCCG
CACAGCCGCCGCCCGCACGACGACGATCACGATCGCCTCTCTGCTCGGCGACGACAAGCCGGAGACGAAGATCTGGGTGA
AAATCGGCGAGCTGGTCGAAGCCAAACTTCCCGGCCAGTTCAAGTTCAATATCGTCAGGAACGGCGCGCTCGGCGGCGAG
AAGGAAGTGGCCGAAGGCGTGCGGCTCGGCTCGATCCAGGCGAGCCTTTCGACGGTGTCGTCGCTGTCCGGCTGGGCGCC
CGAACTGCAGATCCTCGATCTGCCTTTCCTCTTTCGCGATGCCGACCATGTGCGCAGAACTGTCGGCGGCGATGTCGGCG
CCGATCTCAAGCAGAAACTGCAGGCGCAGAATTTCGTCGTCGGCGATTTTATCAATTACGGCGCCCGCCATCTCCTGACC
AAGGAGCCGGTGACGCGACCCGAGCAGCTCAAGGGCAAGCGCATCCGCGTCATCCAGAGCCCGCTTCACACCAAGCTTTG
GAGCGCATTCGGCACGACGCCGATCGGCATTCCGATCACCGAGACCTACAATGCGCTGGCAACCGGCGTCGCCGACGCCA
TGGACCTGACCAAATCGGCTTACTCAGGCTTCAAGCTTTATGAGGTCGTGCCCGATATGACCGAGACAGGCCACATCTGG
GCATCCGGCGTCATCTATTATTCCTCGACCTTCTGGGCCGGCCTGAATGACGAGCAGAAGGCGGTTTTCCAGCAGGCTTC
CAGCGAAGGAGCCGCCTATTTCAACCAGCTGATCGTCGACGACGAGGTAAAGTCCGTGGAAACGGCGCTTGGCCATGGCG
GCAAGCTCTTGAAGCCGGAAGCCTTCGAGGAATGGCAGAAGGGCGCGCAGGGCGTCTGGGCCGATTTCGCGCCTGTTGTC
GGCGGCCTCGACAGGATCAAAACCGTTCAGGCGGCTTGA

Upstream 100 bases:

>100_bases
GAACTCTTCCGGGCCGTCGTTCCCTATCTGCTCGCCCTTCTGGTCTCGCTGGCAATCCTCTGCGCCTGGGCCATCATTTT
CTAGTGAACAGGACAATACC

Downstream 100 bases:

>100_bases
AGCAGGCGAGGGCGGACGCCTGATGTTTCCGACAGTGCCGATTGTAAAAAATCGGCCCTGTCATCGCTTGGATGCATCGG
AAAAATAATGCCGTCGCAGG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNNLNRRNFLRTAALTGTALAAPGFVRTAAARTTTITIASLLGDDKPETKIWVKIGELVEAKLPGQFKFNIVRNGALGGE
KEVAEGVRLGSIQASLSTVSSLSGWAPELQILDLPFLFRDADHVRRTVGGDVGADLKQKLQAQNFVVGDFINYGARHLLT
KEPVTRPEQLKGKRIRVIQSPLHTKLWSAFGTTPIGIPITETYNALATGVADAMDLTKSAYSGFKLYEVVPDMTETGHIW
ASGVIYYSSTFWAGLNDEQKAVFQQASSEGAAYFNQLIVDDEVKSVETALGHGGKLLKPEAFEEWQKGAQGVWADFAPVV
GGLDRIKTVQAA

Sequences:

>Translated_332_residues
MNNLNRRNFLRTAALTGTALAAPGFVRTAAARTTTITIASLLGDDKPETKIWVKIGELVEAKLPGQFKFNIVRNGALGGE
KEVAEGVRLGSIQASLSTVSSLSGWAPELQILDLPFLFRDADHVRRTVGGDVGADLKQKLQAQNFVVGDFINYGARHLLT
KEPVTRPEQLKGKRIRVIQSPLHTKLWSAFGTTPIGIPITETYNALATGVADAMDLTKSAYSGFKLYEVVPDMTETGHIW
ASGVIYYSSTFWAGLNDEQKAVFQQASSEGAAYFNQLIVDDEVKSVETALGHGGKLLKPEAFEEWQKGAQGVWADFAPVV
GGLDRIKTVQAA
>Mature_332_residues
MNNLNRRNFLRTAALTGTALAAPGFVRTAAARTTTITIASLLGDDKPETKIWVKIGELVEAKLPGQFKFNIVRNGALGGE
KEVAEGVRLGSIQASLSTVSSLSGWAPELQILDLPFLFRDADHVRRTVGGDVGADLKQKLQAQNFVVGDFINYGARHLLT
KEPVTRPEQLKGKRIRVIQSPLHTKLWSAFGTTPIGIPITETYNALATGVADAMDLTKSAYSGFKLYEVVPDMTETGHIW
ASGVIYYSSTFWAGLNDEQKAVFQQASSEGAAYFNQLIVDDEVKSVETALGHGGKLLKPEAFEEWQKGAQGVWADFAPVV
GGLDRIKTVQAA

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=235, Percent_Identity=28.936170212766, Blast_Score=124, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 35977; Mature: 35977

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLNRRNFLRTAALTGTALAAPGFVRTAAARTTTITIASLLGDDKPETKIWVKIGELVE
CCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHEEHHHHHCCCCCCCEEEEEEHHHHH
AKLPGQFKFNIVRNGALGGEKEVAEGVRLGSIQASLSTVSSLSGWAPELQILDLPFLFRD
HCCCCCEEEEEEECCCCCCHHHHHCCCEECHHHHHHHHHHHHCCCCCCEEEEECCHHHCC
ADHVRRTVGGDVGADLKQKLQAQNFVVGDFINYGARHLLTKEPVTRPEQLKGKRIRVIQS
HHHHHHHHCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEHHH
PLHTKLWSAFGTTPIGIPITETYNALATGVADAMDLTKSAYSGFKLYEVVPDMTETGHIW
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHCCCCCCCCCEE
ASGVIYYSSTFWAGLNDEQKAVFQQASSEGAAYFNQLIVDDEVKSVETALGHGGKLLKPE
ECCEEEEECEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCH
AFEEWQKGAQGVWADFAPVVGGLDRIKTVQAA
HHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MNNLNRRNFLRTAALTGTALAAPGFVRTAAARTTTITIASLLGDDKPETKIWVKIGELVE
CCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHEEHHHHHCCCCCCCEEEEEEHHHHH
AKLPGQFKFNIVRNGALGGEKEVAEGVRLGSIQASLSTVSSLSGWAPELQILDLPFLFRD
HCCCCCEEEEEEECCCCCCHHHHHCCCEECHHHHHHHHHHHHCCCCCCEEEEECCHHHCC
ADHVRRTVGGDVGADLKQKLQAQNFVVGDFINYGARHLLTKEPVTRPEQLKGKRIRVIQS
HHHHHHHHCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEHHH
PLHTKLWSAFGTTPIGIPITETYNALATGVADAMDLTKSAYSGFKLYEVVPDMTETGHIW
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHCCCCCCCCCEE
ASGVIYYSSTFWAGLNDEQKAVFQQASSEGAAYFNQLIVDDEVKSVETALGHGGKLLKPE
ECCEEEEECEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCH
AFEEWQKGAQGVWADFAPVVGGLDRIKTVQAA
HHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]