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The map label for this gene is rfbD [C]

Identifier: 209548785

GI number: 209548785

Start: 1193089

End: 1193976

Strand: Reverse

Name: rfbD [C]

Synonym: Rleg2_1182

Alternate gene names: 209548785

Gene position: 1193976-1193089 (Counterclockwise)

Preceding gene: 209548786

Following gene: 209548784

Centisome position: 26.31

GC content: 63.4

Gene sequence:

>888_bases
ATGCGCATTGCCGTTACCGGCAAACAGGGCCAGGTCGTCCAGTCGCTGCTCAGACGTGGCGCCGAAACGGGCATCGACAT
CAGCGCGGTCGGGCGCCCGGAAATGGACCTCGCGAATCCCGAAAGCATCGCAGCCGCCTTCTCGGCTCTGCGCCCGGATG
TGATCGTCTCGGCCGCTGCCTATACGGCGGTCGACAAGGCCGAGAGCGAAGCCGAGCTTGCTTTTTCCGTCAACGCGGCA
GGCGCCGGGGCGGTTGCCGAGGCTGCCGCGCGGATCGGGGCCCCGGTCATCCACATTTCGACCGACTACGTCTTCAGCGG
CGACAAGGCCTCCGCCTATTCCGAAGAGGACGCGACTGCGCCGATCTCCGTCTACGGGCGTTCGAAGCTCGCGGGCGAGG
AGGCCGTCGCGGCGGCGAACCCGAACCATGTCATCCTGCGAACCGCCTGGGTCTATTCGCCCTTTGGCACCAATTTCCTG
AAAACGATGCTGCGGCTTTCCGAGACCCGCGATCATCTTCGCGTTGTCGCCGATCAGACGGGATGCCCGACCTCGGCACT
CGATATCGCCGACGCGATTCTTGCGATCGCAGCCCGTGTCGTCGCGGACCCTGCGCCATCGCTCCGCGGTACCTTTCACC
TGACCGGCAGTGGCGAAGCAAGCTGGGCCGATTTCGCCGAAGCGATATTCGCTGAGCTTTCCAAATCCGGCGGCAGAAAC
GTCGGCGTCGAACGCATCCCGACTGCGGACTATCCGACACCGGCAAAGCGTCCCGCCAATTCACGGCTCAACGGCGACAA
GCTCGCCAGAACATATGGACTCCGGCTCCCGGAGTGGAAGCAGTCCATGACGATTGTCATGCAAGACCTTTTGAACAAGG
GTCTTTAA

Upstream 100 bases:

>100_bases
AGACCGTGCACTGGTACTTGGAAAACGAATGGTGGTGGAGGCCGCTGCGCGAAAACATCTACTCCGGCGAACGCCTCGGC
GTTTTCAAGGGACGATAGGG

Downstream 100 bases:

>100_bases
GGAGATGGGCATGAAGGGCATTATTCTCGCCGGCGGCACGGGCACACGTCTGCATCCGATCACCCAGGCGGTCTCGAAAC
AGTTGATGCCGGTCTACGAC

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRIAVTGKQGQVVQSLLRRGAETGIDISAVGRPEMDLANPESIAAAFSALRPDVIVSAAAYTAVDKAESEAELAFSVNAA
GAGAVAEAAARIGAPVIHISTDYVFSGDKASAYSEEDATAPISVYGRSKLAGEEAVAAANPNHVILRTAWVYSPFGTNFL
KTMLRLSETRDHLRVVADQTGCPTSALDIADAILAIAARVVADPAPSLRGTFHLTGSGEASWADFAEAIFAELSKSGGRN
VGVERIPTADYPTPAKRPANSRLNGDKLARTYGLRLPEWKQSMTIVMQDLLNKGL

Sequences:

>Translated_295_residues
MRIAVTGKQGQVVQSLLRRGAETGIDISAVGRPEMDLANPESIAAAFSALRPDVIVSAAAYTAVDKAESEAELAFSVNAA
GAGAVAEAAARIGAPVIHISTDYVFSGDKASAYSEEDATAPISVYGRSKLAGEEAVAAANPNHVILRTAWVYSPFGTNFL
KTMLRLSETRDHLRVVADQTGCPTSALDIADAILAIAARVVADPAPSLRGTFHLTGSGEASWADFAEAIFAELSKSGGRN
VGVERIPTADYPTPAKRPANSRLNGDKLARTYGLRLPEWKQSMTIVMQDLLNKGL
>Mature_295_residues
MRIAVTGKQGQVVQSLLRRGAETGIDISAVGRPEMDLANPESIAAAFSALRPDVIVSAAAYTAVDKAESEAELAFSVNAA
GAGAVAEAAARIGAPVIHISTDYVFSGDKASAYSEEDATAPISVYGRSKLAGEEAVAAANPNHVILRTAWVYSPFGTNFL
KTMLRLSETRDHLRVVADQTGCPTSALDIADAILAIAARVVADPAPSLRGTFHLTGSGEASWADFAEAIFAELSKSGGRN
VGVERIPTADYPTPAKRPANSRLNGDKLARTYGLRLPEWKQSMTIVMQDLLNKGL

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=285, Percent_Identity=29.8245614035088, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=30.4195804195804, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788352, Length=298, Percent_Identity=44.9664429530201, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31009; Mature: 31009

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVTGKQGQVVQSLLRRGAETGIDISAVGRPEMDLANPESIAAAFSALRPDVIVSAAA
CEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
YTAVDKAESEAELAFSVNAAGAGAVAEAAARIGAPVIHISTDYVFSGDKASAYSEEDATA
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCEEECCCCCCCCCCCCCCC
PISVYGRSKLAGEEAVAAANPNHVILRTAWVYSPFGTNFLKTMLRLSETRDHLRVVADQT
CEEECCCCCCCCCHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCC
GCPTSALDIADAILAIAARVVADPAPSLRGTFHLTGSGEASWADFAEAIFAELSKSGGRN
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
VGVERIPTADYPTPAKRPANSRLNGDKLARTYGLRLPEWKQSMTIVMQDLLNKGL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIAVTGKQGQVVQSLLRRGAETGIDISAVGRPEMDLANPESIAAAFSALRPDVIVSAAA
CEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
YTAVDKAESEAELAFSVNAAGAGAVAEAAARIGAPVIHISTDYVFSGDKASAYSEEDATA
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCEEECCCCCCCCCCCCCCC
PISVYGRSKLAGEEAVAAANPNHVILRTAWVYSPFGTNFLKTMLRLSETRDHLRVVADQT
CEEECCCCCCCCCHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCC
GCPTSALDIADAILAIAARVVADPAPSLRGTFHLTGSGEASWADFAEAIFAELSKSGGRN
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
VGVERIPTADYPTPAKRPANSRLNGDKLARTYGLRLPEWKQSMTIVMQDLLNKGL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]