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The map label for this gene is rffG [C]

Identifier: 209548786

GI number: 209548786

Start: 1193979

End: 1195034

Strand: Reverse

Name: rffG [C]

Synonym: Rleg2_1183

Alternate gene names: 209548786

Gene position: 1195034-1193979 (Counterclockwise)

Preceding gene: 209548787

Following gene: 209548785

Centisome position: 26.33

GC content: 60.04

Gene sequence:

>1056_bases
ATGCGCATACTGGTCACGGGCGGAGCGGGCTTCATCGGATCGGCATTGGTGCGCCATCTCGTCAGCGAGATCGGCGCCGA
GGTGCTGAATGTCGACACGCTGACCTACGCCGGAAATCTGGCATCGCTGAAATCCGTCGAATCGGCGCCGAACTATCAAT
TCCTGCGCGCCGACATCTGCGACCGCACCGGAATGCAGGAAGCATTCGCGTCCTTCCGCCCCGACATCGTCATGCATCTC
GCGGCCGAGAGCCATGTAGACCGCTCGATTTCGGGGGCGGCCGATTTCATTCAGACCAACATCGTCGGCACATTCAGCCT
GCTGGATGCCGCACGACTTTACTGGGATGGGCTTGACGCTCCGCGCAAGAGCGCCTTCCGTTTTCTGCATGTCTCGACGG
ACGAGGTCTACGGCTCGCTCGGCGATGAGGGCCTCTTCGAGGAGACGACGCCTTACGATCCGTCCTCGCCCTACTCCGCC
TCCAAGGCCGCGAGCGACCACCTGGCGATCGCCTGGCACCGCACCTACGGCCTGCCCGTCGTCGTCTCCAACTGCTCCAA
CAACTACGGACCGTTTCATTTCCCGGAAAAGCTCATTCCGCTGATGATCCTGAACGCATTGGAGGGCAAGCCTCTTCCGG
TTTACGGCAATGGCGTGAATGTCCGCGACTGGCTCTATGTCGAAGACCACGCCCGCGCGCTCTTCACGATCGCATCCAGA
GGGCGTCCCGGCGAAAAATACAATGTAGGCGGCTGCAATGAGCGCAGGAACATCGATGTCGTTCATCGCATCTGCGCTAT
TCTCGACGGCGTCTTTAGTGACAAGGCACCGCATGCGCGTCTGATCACCAACGTCACGGATCGCCCCGGACACGACGCAC
GTTATGCAATCGATGCCTCGAAACTCGAAAGCGAACTCGGGTGGAAGGCGCAAGAGACCTTCGAAACCGGCATCGAGAAG
ACCGTGCACTGGTACTTGGAAAACGAATGGTGGTGGAGGCCGCTGCGCGAAAACATCTACTCCGGCGAACGCCTCGGCGT
TTTCAAGGGACGATAG

Upstream 100 bases:

>100_bases
AGACATGGTATCCTCCGACAAGGACAAGACGCTGCCGCGGCTTGCCGATTTGCCAGGTCATTTTTCTTTTTCAACACAGC
AACCCCAGGATTGATCGACG

Downstream 100 bases:

>100_bases
GGATGCGCATTGCCGTTACCGGCAAACAGGGCCAGGTCGTCCAGTCGCTGCTCAGACGTGGCGCCGAAACGGGCATCGAC
ATCAGCGCGGTCGGGCGCCC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRILVTGGAGFIGSALVRHLVSEIGAEVLNVDTLTYAGNLASLKSVESAPNYQFLRADICDRTGMQEAFASFRPDIVMHL
AAESHVDRSISGAADFIQTNIVGTFSLLDAARLYWDGLDAPRKSAFRFLHVSTDEVYGSLGDEGLFEETTPYDPSSPYSA
SKAASDHLAIAWHRTYGLPVVVSNCSNNYGPFHFPEKLIPLMILNALEGKPLPVYGNGVNVRDWLYVEDHARALFTIASR
GRPGEKYNVGGCNERRNIDVVHRICAILDGVFSDKAPHARLITNVTDRPGHDARYAIDASKLESELGWKAQETFETGIEK
TVHWYLENEWWWRPLRENIYSGERLGVFKGR

Sequences:

>Translated_351_residues
MRILVTGGAGFIGSALVRHLVSEIGAEVLNVDTLTYAGNLASLKSVESAPNYQFLRADICDRTGMQEAFASFRPDIVMHL
AAESHVDRSISGAADFIQTNIVGTFSLLDAARLYWDGLDAPRKSAFRFLHVSTDEVYGSLGDEGLFEETTPYDPSSPYSA
SKAASDHLAIAWHRTYGLPVVVSNCSNNYGPFHFPEKLIPLMILNALEGKPLPVYGNGVNVRDWLYVEDHARALFTIASR
GRPGEKYNVGGCNERRNIDVVHRICAILDGVFSDKAPHARLITNVTDRPGHDARYAIDASKLESELGWKAQETFETGIEK
TVHWYLENEWWWRPLRENIYSGERLGVFKGR
>Mature_351_residues
MRILVTGGAGFIGSALVRHLVSEIGAEVLNVDTLTYAGNLASLKSVESAPNYQFLRADICDRTGMQEAFASFRPDIVMHL
AAESHVDRSISGAADFIQTNIVGTFSLLDAARLYWDGLDAPRKSAFRFLHVSTDEVYGSLGDEGLFEETTPYDPSSPYSA
SKAASDHLAIAWHRTYGLPVVVSNCSNNYGPFHFPEKLIPLMILNALEGKPLPVYGNGVNVRDWLYVEDHARALFTIASR
GRPGEKYNVGGCNERRNIDVVHRICAILDGVFSDKAPHARLITNVTDRPGHDARYAIDASKLESELGWKAQETFETGIEK
TVHWYLENEWWWRPLRENIYSGERLGVFKGR

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=35.1351351351351, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=25.1515151515152, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4504031, Length=351, Percent_Identity=25.6410256410256, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=63.3522727272727, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1788353, Length=360, Percent_Identity=60.8333333333333, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=26.7806267806268, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788366, Length=249, Percent_Identity=27.3092369477912, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=35.3293413173653, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=332, Percent_Identity=29.8192771084337, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71982035, Length=373, Percent_Identity=27.3458445040214, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=375, Percent_Identity=27.2, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=24.2424242424242, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI133901786, Length=252, Percent_Identity=25.7936507936508, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=252, Percent_Identity=25.7936507936508, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=252, Percent_Identity=25.7936507936508, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=252, Percent_Identity=25.7936507936508, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=252, Percent_Identity=25.7936507936508, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=252, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=24.9315068493151, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=26.0606060606061, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19923002, Length=372, Percent_Identity=27.4193548387097, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39064; Mature: 39064

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGAGFIGSALVRHLVSEIGAEVLNVDTLTYAGNLASLKSVESAPNYQFLRADIC
CEEEEECCCCHHHHHHHHHHHHHHCHHHCCCCHHEECCCHHHHHHHHCCCCCEEEEHHHH
DRTGMQEAFASFRPDIVMHLAAESHVDRSISGAADFIQTNIVGTFSLLDAARLYWDGLDA
HCCCHHHHHHHCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRKSAFRFLHVSTDEVYGSLGDEGLFEETTPYDPSSPYSASKAASDHLAIAWHRTYGLPV
CCHHHEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEEEECCCCEE
VVSNCSNNYGPFHFPEKLIPLMILNALEGKPLPVYGNGVNVRDWLYVEDHARALFTIASR
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECHHHEEEHHHHC
GRPGEKYNVGGCNERRNIDVVHRICAILDGVFSDKAPHARLITNVTDRPGHDARYAIDAS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCCCCCEEEEEHH
KLESELGWKAQETFETGIEKTVHWYLENEWWWRPLRENIYSGERLGVFKGR
HHHHHHCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHCCCCEEECCCCC
>Mature Secondary Structure
MRILVTGGAGFIGSALVRHLVSEIGAEVLNVDTLTYAGNLASLKSVESAPNYQFLRADIC
CEEEEECCCCHHHHHHHHHHHHHHCHHHCCCCHHEECCCHHHHHHHHCCCCCEEEEHHHH
DRTGMQEAFASFRPDIVMHLAAESHVDRSISGAADFIQTNIVGTFSLLDAARLYWDGLDA
HCCCHHHHHHHCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PRKSAFRFLHVSTDEVYGSLGDEGLFEETTPYDPSSPYSASKAASDHLAIAWHRTYGLPV
CCHHHEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEEEECCCCEE
VVSNCSNNYGPFHFPEKLIPLMILNALEGKPLPVYGNGVNVRDWLYVEDHARALFTIASR
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECHHHEEEHHHHC
GRPGEKYNVGGCNERRNIDVVHRICAILDGVFSDKAPHARLITNVTDRPGHDARYAIDAS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCCCCCEEEEEHH
KLESELGWKAQETFETGIEKTVHWYLENEWWWRPLRENIYSGERLGVFKGR
HHHHHHCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]