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The map label for this gene is irlS [H]

Identifier: 209548308

GI number: 209548308

Start: 727263

End: 728660

Strand: Reverse

Name: irlS [H]

Synonym: Rleg2_0703

Alternate gene names: 209548308

Gene position: 728660-727263 (Counterclockwise)

Preceding gene: 209548309

Following gene: 209548305

Centisome position: 16.06

GC content: 63.23

Gene sequence:

>1398_bases
ATGCCGTCCGAAAACCGCACACATTTTTCCGCGTCATTCTCCAGCCTGCGCCGGAGCACGCCGTTCCGGCTTGCCGTCAC
CTTCGTCGTGCTCTTCGTCGTCACCTTCTTTTTGAGCGGCGCGATCATCTATCACATGCTGGACCTCGGCCTCTCCCGCG
ATCTCGAACAGTCGCTCACCGAGATGAATGCGTTGATCGTTTCGACCTACGAGCCCGGCGACACCCAGGATCTCGTCAAC
ACGCTCAACAATTATGCGAGCTTCCAGTCTACCTCCGACGGGCTTTATTCGCTGACCGATCATGCCGGCAAGAAACTCGC
CGGCAATTTCGCCGCACCGCCCATCCCGAACGGCGTCTATACCGTCACCTCCCGCGATGTCGGGCTGAAGGGCCATGAAC
GCTACCGGATGCAGGTGTCGACGATCGGCCCCTACAGCCTGGTGGTCGGCGAAAACTTCAACGATGTCGAGGAGATGCTG
CGGATCGTGCTGGTGAGTTTCGAATGGGCCGCGGCAATCGTCGTGGCGACGGCAATCGGCGGCGGCGTGTTTCTCGCCGT
TCGCGCCCAGGCGCGGCTGGACAGGGTCGCGCTCACCATGAACGATGTCTCCCACGGCGCGCTCGACGCCCGCATTCCGA
TCAGCGGCAATGGCGATGATCTCGATACGGTGGCGATCCAGATCAACGCGGCGCTGGTGCGGCTGCAGACCTTGGTCGAG
AGCATGCGGCAGGTGAGCAGCGATATCGCCCATGATCTGAAGACACCGCTCAACCGGCTGCGCCTGACGCTCGATGCCGC
GGTGGCGGGCAATGACCGGCAGGCGGATGTCTCAGCCCTTCTCGACGAAGCGAGACACGAGAGCGACCGGATCAACGCCA
CCTTCGAGGCGCTGCTGCGCATCTCCCAGATCGAGGCCGGCGCCCGCAAGGAGCGTTTCCAGGCGACCGATCTCGATGCC
GTGCTTGCGGTGATTTCCGAAGTCTATGTCGATGTCGCCGAAGATGCGCAGAAGTCGCTGCGGATCACGGCGCGCTGTCC
GGCGATGATCCGCGGCGATCGCGATCTGCTGACCCAGATGCTCGCCAACCTGGTGGAAAATGCGATCAACCACTGCCCGG
CCGGAACGGCGATCACCGTTTCGCTCAGCCGCCAGCACGATCGCGCCATCCTCTCGGTCGCCGATACCGGCCCCGGCATT
CCCGCCGGCGAAAGGGAAAAAGTCTTCCGGCGGCTCTACCGGCTGGACAAAAGCCGCACGACATCAGGAAGCGGGCTCGG
GCTCAGCCTCGTCAAGGCGATCGCCGAGCTGCATGCGGCTGAGATCACCATGGGAGACAATCAGCCCGGCCTCATCGTCT
CGATCGGCTTTCCGCTGCTCCCGGGTGAGCGGTCCTGA

Upstream 100 bases:

>100_bases
TCAGCCGTTTGCGGGCCAAGGTCGACAGACCGTTCCAGGCCCAGCTTCTGCACACCGTCCGCAACACCGGGTACAGCCTG
CACGCGCCCAGGACAGGATG

Downstream 100 bases:

>100_bases
CCTTTCATCCCGGGTGAGCGGTCTTGACCGCTCACCCTCCACGCCACGAGGCGGCCGAAGCGGGCGGCAAAGCGGCGTAT
TTCGGCCGCCGCGCGATAGG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPSENRTHFSASFSSLRRSTPFRLAVTFVVLFVVTFFLSGAIIYHMLDLGLSRDLEQSLTEMNALIVSTYEPGDTQDLVN
TLNNYASFQSTSDGLYSLTDHAGKKLAGNFAAPPIPNGVYTVTSRDVGLKGHERYRMQVSTIGPYSLVVGENFNDVEEML
RIVLVSFEWAAAIVVATAIGGGVFLAVRAQARLDRVALTMNDVSHGALDARIPISGNGDDLDTVAIQINAALVRLQTLVE
SMRQVSSDIAHDLKTPLNRLRLTLDAAVAGNDRQADVSALLDEARHESDRINATFEALLRISQIEAGARKERFQATDLDA
VLAVISEVYVDVAEDAQKSLRITARCPAMIRGDRDLLTQMLANLVENAINHCPAGTAITVSLSRQHDRAILSVADTGPGI
PAGEREKVFRRLYRLDKSRTTSGSGLGLSLVKAIAELHAAEITMGDNQPGLIVSIGFPLLPGERS

Sequences:

>Translated_465_residues
MPSENRTHFSASFSSLRRSTPFRLAVTFVVLFVVTFFLSGAIIYHMLDLGLSRDLEQSLTEMNALIVSTYEPGDTQDLVN
TLNNYASFQSTSDGLYSLTDHAGKKLAGNFAAPPIPNGVYTVTSRDVGLKGHERYRMQVSTIGPYSLVVGENFNDVEEML
RIVLVSFEWAAAIVVATAIGGGVFLAVRAQARLDRVALTMNDVSHGALDARIPISGNGDDLDTVAIQINAALVRLQTLVE
SMRQVSSDIAHDLKTPLNRLRLTLDAAVAGNDRQADVSALLDEARHESDRINATFEALLRISQIEAGARKERFQATDLDA
VLAVISEVYVDVAEDAQKSLRITARCPAMIRGDRDLLTQMLANLVENAINHCPAGTAITVSLSRQHDRAILSVADTGPGI
PAGEREKVFRRLYRLDKSRTTSGSGLGLSLVKAIAELHAAEITMGDNQPGLIVSIGFPLLPGERS
>Mature_464_residues
PSENRTHFSASFSSLRRSTPFRLAVTFVVLFVVTFFLSGAIIYHMLDLGLSRDLEQSLTEMNALIVSTYEPGDTQDLVNT
LNNYASFQSTSDGLYSLTDHAGKKLAGNFAAPPIPNGVYTVTSRDVGLKGHERYRMQVSTIGPYSLVVGENFNDVEEMLR
IVLVSFEWAAAIVVATAIGGGVFLAVRAQARLDRVALTMNDVSHGALDARIPISGNGDDLDTVAIQINAALVRLQTLVES
MRQVSSDIAHDLKTPLNRLRLTLDAAVAGNDRQADVSALLDEARHESDRINATFEALLRISQIEAGARKERFQATDLDAV
LAVISEVYVDVAEDAQKSLRITARCPAMIRGDRDLLTQMLANLVENAINHCPAGTAITVSLSRQHDRAILSVADTGPGIP
AGEREKVFRRLYRLDKSRTTSGSGLGLSLVKAIAELHAAEITMGDNQPGLIVSIGFPLLPGERS

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790861, Length=291, Percent_Identity=29.2096219931272, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786600, Length=215, Percent_Identity=30.2325581395349, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788279, Length=208, Percent_Identity=31.25, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786783, Length=491, Percent_Identity=23.8289205702648, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789403, Length=461, Percent_Identity=26.0303687635575, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787894, Length=288, Percent_Identity=28.8194444444444, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=28.3582089552239, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790551, Length=274, Percent_Identity=29.1970802919708, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=27.9352226720648, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789808, Length=278, Percent_Identity=27.3381294964029, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786912, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787374, Length=237, Percent_Identity=25.7383966244726, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=27.0386266094421, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50370; Mature: 50239

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSENRTHFSASFSSLRRSTPFRLAVTFVVLFVVTFFLSGAIIYHMLDLGLSRDLEQSLT
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EMNALIVSTYEPGDTQDLVNTLNNYASFQSTSDGLYSLTDHAGKKLAGNFAAPPIPNGVY
HHHEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHCCCCCCCCCCCCEE
TVTSRDVGLKGHERYRMQVSTIGPYSLVVGENFNDVEEMLRIVLVSFEWAAAIVVATAIG
EEECCCCCCCCCCEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGVFLAVRAQARLDRVALTMNDVSHGALDARIPISGNGDDLDTVAIQINAALVRLQTLVE
CCEEEEEEHHHHHHHHEEEECCCCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHH
SMRQVSSDIAHDLKTPLNRLRLTLDAAVAGNDRQADVSALLDEARHESDRINATFEALLR
HHHHHHHHHHHHHHHHHHHHEEEEEHEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
ISQIEAGARKERFQATDLDAVLAVISEVYVDVAEDAQKSLRITARCPAMIRGDRDLLTQM
HHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCHHHHHHH
LANLVENAINHCPAGTAITVSLSRQHDRAILSVADTGPGIPAGEREKVFRRLYRLDKSRT
HHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
TSGSGLGLSLVKAIAELHAAEITMGDNQPGLIVSIGFPLLPGERS
CCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
PSENRTHFSASFSSLRRSTPFRLAVTFVVLFVVTFFLSGAIIYHMLDLGLSRDLEQSLT
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EMNALIVSTYEPGDTQDLVNTLNNYASFQSTSDGLYSLTDHAGKKLAGNFAAPPIPNGVY
HHHEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHCCCCCCCCCCCCEE
TVTSRDVGLKGHERYRMQVSTIGPYSLVVGENFNDVEEMLRIVLVSFEWAAAIVVATAIG
EEECCCCCCCCCCEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGVFLAVRAQARLDRVALTMNDVSHGALDARIPISGNGDDLDTVAIQINAALVRLQTLVE
CCEEEEEEHHHHHHHHEEEECCCCCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHHH
SMRQVSSDIAHDLKTPLNRLRLTLDAAVAGNDRQADVSALLDEARHESDRINATFEALLR
HHHHHHHHHHHHHHHHHHHHEEEEEHEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
ISQIEAGARKERFQATDLDAVLAVISEVYVDVAEDAQKSLRITARCPAMIRGDRDLLTQM
HHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCHHHHHHH
LANLVENAINHCPAGTAITVSLSRQHDRAILSVADTGPGIPAGEREKVFRRLYRLDKSRT
HHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
TSGSGLGLSLVKAIAELHAAEITMGDNQPGLIVSIGFPLLPGERS
CCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]