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The map label for this gene is hyfG [H]

Identifier: 209399980

GI number: 209399980

Start: 3428699

End: 3430414

Strand: Direct

Name: hyfG [H]

Synonym: ECH74115_3709

Alternate gene names: 209399980

Gene position: 3428699-3430414 (Clockwise)

Preceding gene: 209400562

Following gene: 209399054

Centisome position: 61.53

GC content: 54.6

Gene sequence:

>1716_bases
GTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGA
TGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATT
ATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCC
CTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGTGGTGAAGGCGCTGGTCGATGCCGACAGTCGGGAGTTTCCGTCAGT
CACACCGCGCGTCCCTGCCGCGGTCTGGGGCGAGCGAGAAATTCGTGATATGTACGGGCTGATTCCGGTTGGCCTGCCGG
ATCAGCGTCGCCTGGTGTTGCCCGATGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTG
CGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGGTGCGCGGGTGATCCCTGTCGG
CCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACC
GTCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTCTTATCGGAC
CGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAACTCGGTTGAAAATGCACTGGGGATTGAGGTGCC
GCAACGAGCGCATACCATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTCAACCTTGGCCTCTCCT
GCCATTTTGTTGGATTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACAATGGCGGAATTGCTG
ACCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAAC
GCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAAATGCTGCTTGCCACGCCGAATATGGAACAAC
GCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTATAGCCCTGTAGGGCCGCTGATCCGCGGCAGTGGT
TTTGCCCGTGATTTGCGCTTTGATCACCCCTACGCCGACTACGGCAATATTCCAAAAACACTGTTTACCTTCACCGGCGG
CGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGGAATTTGCTCTCGACAACA
TGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTCGGCTTTGTTGAAGCGCCACGC
GGTGAAGACGTGCACTGGAGCATGCTCGGTGATAACCAAAAATTGTTCCGCTGGCGCTGCCGTGCCGCCACCTACGCCAA
CTGGCCGGTGTTGCGTTACATGCTGCGCGGCAATACCGTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCT
ACTCCTGTACCGACCGTGTGACGCTGGTTGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGC
TACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAA

Upstream 100 bases:

>100_bases
TCCACTATAGTCCTCTCAGGGACGCACGACCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGC
ATCTGTTTCGGAGAAACACA

Downstream 100 bases:

>100_bases
GGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGA
GGTCAGCCCAGGCTTTCGCG

Product: hydrogenase-4, G subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIER
YGIDRNRSPLK

Sequences:

>Translated_571_residues
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIER
YGIDRNRSPLK
>Mature_571_residues
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYA
LSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELL
TGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSG
FARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIER
YGIDRNRSPLK

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=381, Percent_Identity=26.509186351706, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI260898743, Length=371, Percent_Identity=26.4150943396226, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1788832, Length=571, Percent_Identity=96.8476357267951, Blast_Score=1135, Evalue=0.0,
Organism=Escherichia coli, GI1789076, Length=568, Percent_Identity=72.3591549295775, Blast_Score=892, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=539, Percent_Identity=27.0871985157699, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17555284, Length=392, Percent_Identity=26.0204081632653, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17568379, Length=392, Percent_Identity=25.5102040816327, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI221459469, Length=386, Percent_Identity=26.6839378238342, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24638644, Length=380, Percent_Identity=27.8947368421053, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 64988; Mature: 64988

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCE
PVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGERE
EEEECCCCCEECCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHH
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYPFINEGNSG
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCC
ARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
CEEEEECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHH
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
HHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
HHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKT
HHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRV
CCCEEEEEECCCHHHHHEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCE
TLVDVRKRQSKTVPYKEIERYGIDRNRSPLK
EEEEEHHHHCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWL
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCE
PVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGERE
EEEECCCCCEECCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHH
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYPFINEGNSG
HHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCC
ARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
CEEEEECCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHH
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
HHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
TGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
HHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVEMLLATPNMEQRTQGIGILDRQIARDYSPVGPLIRGSGFARDLRFDHPYADYGNIPKT
HHHHHHCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSYKPHAFALGFVEAPR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
GEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRV
CCCEEEEEECCCHHHHHEEHHHCCCCCCHHHHHHHCCCCCCCCCEEEECCCCHHCCCCCE
TLVDVRKRQSKTVPYKEIERYGIDRNRSPLK
EEEEEHHHHCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]