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The map label for this gene is hyfH [H]

Identifier: 209399054

GI number: 209399054

Start: 3430424

End: 3430969

Strand: Direct

Name: hyfH [H]

Synonym: ECH74115_3710

Alternate gene names: 209399054

Gene position: 3430424-3430969 (Clockwise)

Preceding gene: 209399980

Following gene: 209398464

Centisome position: 61.56

GC content: 55.68

Gene sequence:

>546_bases
ATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCC
AGGCTTTCGCGGGAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGTGGTGCCTGCGCCTGTGCTTGTCCGACAAATG
CGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGT
TGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTA
TACCCGCGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCGTCGCACTGGCTG
CTGAATTGTTGGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGCGGGCGCAAGCGAGCGTCTGCCCGGAGTGC
AAACAACGCGCGACGCTGATCAACGACGATACAGATGTACCGCTGGTGGCTAAGGAGCAGCTATGA

Upstream 100 bases:

>100_bases
GGTTGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGC
CGCTGAAGTAAGGACAGAAG

Downstream 100 bases:

>100_bases
GTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGAGCAAACGCAAAAGATGAAGCGGCATTTGCTACAG
GATATCCGTCGCTCAGCCTA

Product: hydrogenase 4 subunit H

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGR
CEEVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
KQRATLINDDTDVPLVAKEQL

Sequences:

>Translated_181_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGR
CEEVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
KQRATLINDDTDVPLVAKEQL
>Mature_181_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGR
CEEVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
KQRATLINDDTDVPLVAKEQL

Specific function: Probable electron transfer protein for hydrogenase 4 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=106, Percent_Identity=39.622641509434, Blast_Score=70, Evalue=9e-13,
Organism=Escherichia coli, GI1788833, Length=181, Percent_Identity=97.7900552486188, Blast_Score=328, Evalue=1e-91,
Organism=Escherichia coli, GI1789075, Length=170, Percent_Identity=45.2941176470588, Blast_Score=160, Evalue=4e-41,
Organism=Escherichia coli, GI1788617, Length=106, Percent_Identity=37.7358490566038, Blast_Score=74, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17555194, Length=133, Percent_Identity=34.5864661654135, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17864306, Length=106, Percent_Identity=40.5660377358491, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20129; Mature: 20129

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPTNALTIQTD
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHCCHHHCCCCCEEEEEEC
DQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRC
CCCCCEEEEEEECCEEECCCCHHHCCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHC
ERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQRATLINDDTDVPLVAKEQ
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCEECCCCCCCCEEECCC
L
C
>Mature Secondary Structure
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPTNALTIQTD
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHCCHHHCCCCCEEEEEEC
DQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKADLYTRATFHLQRCSRC
CCCCCEEEEEEECCEEECCCCHHHCCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHC
ERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQRATLINDDTDVPLVAKEQ
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCEECCCCCCCCEEECCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]