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The map label for this gene is nrfF

Identifier: 209398766

GI number: 209398766

Start: 5221513

End: 5221896

Strand: Direct

Name: nrfF

Synonym: ECH74115_5580

Alternate gene names: 209398766

Gene position: 5221513-5221896 (Clockwise)

Preceding gene: 209397457

Following gene: 209398536

Centisome position: 93.71

GC content: 52.34

Gene sequence:

>384_bases
ATGTATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGCGCTCAGGTTGTAGACACCTGGCAATTCGC
CAATCCGCAACAACAGCAACAGGCGTTAAATATTGCCAGCCAGTTACGTTGTCCGCAGTGCCAGAATCAAAACTTACTGG
AATCCAACGCGCCGGTGGCAGTCAGTATGCGCCATCAGGTTTACAGCATGGTGGCGGAGGGGAAAAGTGAAGTCGAAATC
ATTGGCTGGATGACCGAACGCTACGGAGATTTTGTTCGCTATAACCCACCGTTAACGGGTCAGACGCTGGTGTTGTGGGC
GCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGA

Upstream 100 bases:

>100_bases
TTTCGCTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGG
ATGGCGGGGGAAGAAGCGCG

Downstream 100 bases:

>100_bases
AACAGCCTCAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTCCTCTGTATCGGTAGTTATCTGTTA
AGCCCAAAATGGCAGGCGGT

Product: formate-dependent nitrite reductase complex subunit NrfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVRAKR

Sequences:

>Translated_127_residues
MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVRAKR
>Mature_127_residues
MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEI
IGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVRAKR

Specific function: Not required for the biosynthesis of any of the c-type cytochromes. Possible subunit of a heme lyase

COG id: COG3088

COG function: function code O; Uncharacterized protein involved in biosynthesis of c-type cytochromes

Gene ontology:

Cell location: Periplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmH/CycL/Ccl2/NrfF family

Homologues:

Organism=Escherichia coli, GI1790512, Length=127, Percent_Identity=97.6377952755905, Blast_Score=190, Evalue=3e-50,
Organism=Escherichia coli, GI1788522, Length=117, Percent_Identity=40.1709401709402, Blast_Score=108, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFF_ECO57 (Q8X5S5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98261
- PIR:   E86101
- RefSeq:   NP_290708.1
- RefSeq:   NP_313084.1
- ProteinModelPortal:   Q8X5S5
- SMR:   Q8X5S5
- EnsemblBacteria:   EBESCT00000025164
- EnsemblBacteria:   EBESCT00000055553
- GeneID:   914285
- GeneID:   960049
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5674
- KEGG:   ecs:ECs5057
- GeneTree:   EBGT00050000008887
- HOGENOM:   HBG758623
- OMA:   IRKQRIG
- ProtClustDB:   PRK10144
- BioCyc:   ECOL83334:ECS5057-MONOMER
- InterPro:   IPR005616
- InterPro:   IPR017565
- TIGRFAMs:   TIGR03147

Pfam domain/function: PF03918 CcmH

EC number: NA

Molecular weight: Translated: 14564; Mature: 14564

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
VSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALIL
EHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
WRVRAKR
HHHHCCC
>Mature Secondary Structure
MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
VSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALIL
EHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
WRVRAKR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796