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The map label for this gene is nrfE [H]

Identifier: 209397457

GI number: 209397457

Start: 5219841

End: 5221520

Strand: Direct

Name: nrfE [H]

Synonym: ECH74115_5579

Alternate gene names: 209397457

Gene position: 5219841-5221520 (Clockwise)

Preceding gene: 209398055

Following gene: 209398766

Centisome position: 93.68

GC content: 56.61

Gene sequence:

>1680_bases
TTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTTGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCG
ACTCACTTGCATCGGCATTCTGGCACAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCA
GCGATTTCTCGGTCATTTATGTCGCCCAACATAGCTACAGCTTGCTGTCGTGGAAACTCAAACTGGCGGCGGTGTGGGGC
GGTCATGAAGGTTCGCTGCTGCTTTGGGTGCTGCTGCTTTCCGCCTGGAGCGCGCTGTTTGCCTGGCAATATCGGCAGCA
AACCGATCCGCTATTTCCGCTGACGCTAGCCGTTTTATCTCTCATGCTCGCCGCACTGCTACTGTTTGTAGTGCTGTGGT
CCGATCCCTTCGTGCGGATATTTCCACCAGCAATCGAAGGCCGCGATCTCAATCCGATGCTGCAACATCCCGGTCTTATC
TTTCATCCACCGCTGCTTTACCTTGGCTATGGCGGTTTGATGGTAGCGGCGAGCGTGGCGCTGGCGAGTCTACTGCGCGG
CGAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACTGCGGGGATCATCC
TCGGTTCCTGGTGGGCCTACTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTA
CCCTGGCTTTCTGCCACTGCGCTGCTGCACAGTTTATCCCTGACACGCCAGCGGGGGATTTTTCGCCACTGGTCGCTGTT
GTTGGCGATAGTTACTCTGATGCTGTCGCTGCTGGGCACCTTAATTGTCCGTTCTGGCATTCTGGTTTCGGTTCATGCGT
TCGCTCTGGATAACGTTCGCGCTGTGCCGTTGTTCAGCCTGTTTGCACTGATTAGCCTTGCGTCTCTGGCTCTGTATGGC
TGGCGAGCGCGGGACGGTGGCCCGGCGGTGCGTTTTTCGGGGTTATCGCGGGAAATGTTAATCCTCGCTACGCTGTTGCT
GTTTTGCGCAGTGCTACTGATCGTGCTGGTGGGAACGCTTTATCCGATGATTTACGGCCTGCTGGGCTGGGGACGTCTCT
CCGTTGGCGCACCGTATTTTAACCGCGCGACGTTACCGTTTGGTCTGTTGATGTTGGTGGTGATTGTGCTGGCGACGTTT
GTCTCTGGCAAACGCGCGCAGCTTCCGGCGCTGCTGGCGCATGCGGGCATACTGTTATTTGCCGCAGGGATCGTTGTCTC
CAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTG
AGCGCCTCGATCTGCAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATT
GGTGAATTAACGCCGGAGCGGCGTTTTTACGAAGCTCGTCGTCAGCAAATGATGGAACCGTCAATTCGCTGGAACGGCAT
CCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGAGCGGATCGTTACGCTTTTCGCTTGTATGTACAAAGCGGTGTGC
GCTGGATCTGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGTATAA

Upstream 100 bases:

>100_bases
CGATTTTTCATTCTCTACGCCGGGCAGTTAACGGTGGCGTAAGCCAGAACAGAGGTGGTTTCTGGACGTATTCCTTCCTG
AAGTCGGTTTTCTGGCGCTG

Downstream 100 bases:

>100_bases
AGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGCGCTCAGGTTGTAGACACCTGGCAATTCGCCAATCCGC
AACAACAGCAACAGGCGTTA

Product: heme lyase subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWG
GHEGSLLLWVLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLI
FHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLL
PWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYG
WRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATF
VSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRI
GELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGKKRDV

Sequences:

>Translated_559_residues
MLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWG
GHEGSLLLWVLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLI
FHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLL
PWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYG
WRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATF
VSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRI
GELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGKKRDV
>Mature_559_residues
MLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWG
GHEGSLLLWVLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLI
FHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLL
PWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYG
WRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATF
VSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRI
GELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWRGKKRDV

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=551, Percent_Identity=98.0036297640653, Blast_Score=1064, Evalue=0.0,
Organism=Escherichia coli, GI1788524, Length=585, Percent_Identity=38.6324786324786, Blast_Score=336, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 62051; Mature: 62051

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIY
CCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VAQHSYSLLSWKLKLAAVWGGHEGSLLLWVLLLSAWSALFAWQYRQQTDPLFPLTLAVLS
EECCCHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVA
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHCCCEEECCCHHHHCCHHHHHHHHHH
LASLLRGEFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLL
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCC
PWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHCCCC
AVPLFSLFALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
YPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLF
HHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRI
HHHHHHHHCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHH
GELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGG
HCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHEEEEHHHCCEEEECCC
LLMIAGALLSGWRGKKRDV
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLSLGVNVLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIY
CCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VAQHSYSLLSWKLKLAAVWGGHEGSLLLWVLLLSAWSALFAWQYRQQTDPLFPLTLAVLS
EECCCHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LMLAALLLFVVLWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVA
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHCCCEEECCCHHHHCCHHHHHHHHHH
LASLLRGEFDGACARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLL
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCC
PWLSATALLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHCCCC
AVPLFSLFALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
YPMIYGLLGWGRLSVGAPYFNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLF
HHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRI
HHHHHHHHCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHH
GELTPERRFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGG
HCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHEEEEHHHCCEEEECCC
LLMIAGALLSGWRGKKRDV
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]