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The map label for this gene is nrfG

Identifier: 209398536

GI number: 209398536

Start: 5221893

End: 5222489

Strand: Direct

Name: nrfG

Synonym: ECH74115_5581

Alternate gene names: 209398536

Gene position: 5221893-5222489 (Clockwise)

Preceding gene: 209398766

Following gene: 209399923

Centisome position: 93.72

GC content: 52.09

Gene sequence:

>597_bases
ATGAAACAGCCTCAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTCCTCTGTATCGGTAGTTATCT
GTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAA
ACCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGC
GAGTACTATCTGTGGCAAAACGATTACAGCAATTCGCTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGC
TGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCG
ACAAAGCCCTCGCGCTGGACTCTAACGAAATCACCGCTCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAATTAC
GCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTCACCCCGGATCAACCGGACACAGCTGGTCGAGTCGAT
TAATATGGCGAAATTGTTGCAGCGGAGATCGGATTAA

Upstream 100 bases:

>100_bases
ATAACCCACCGTTAACGGGTCAGACGCTGGTGTTGTGGGCGCTGCCAGTGGTGTTGTTACTGCTGATGGCACTGATCCTC
TGGCGAGTGAGGGCGAAGCG

Downstream 100 bases:

>100_bases
TCATCTGGTGATGAAGATTATGTTATTGAGCTGCATAAAAAATAATCGAATGAACATATGCCAAAAATAATCACTAATCA
GTATTATTGCAGATTAAACA

Product: formate-dependent nitrite reductase complex subunit NrfG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRDPLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLG
EYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD

Sequences:

>Translated_198_residues
MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRDPLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLG
EYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD
>Mature_198_residues
MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRDPLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLG
EYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD

Specific function: Required for formate-dependent nitrite reduction. Not required for the biosynthesis of any of the c-type cytochromes nor for the secretion of the periplasmic cytochromes

COG id: COG4235

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 TPR repeats

Homologues:

Organism=Escherichia coli, GI1790513, Length=198, Percent_Identity=97.4747474747475, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFG_ECO57 (Q8X5S3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91261
- PIR:   F86101
- RefSeq:   NP_290709.1
- RefSeq:   NP_313085.1
- PDB:   2E2E
- PDBsum:   2E2E
- ProteinModelPortal:   Q8X5S3
- SMR:   Q8X5S3
- EnsemblBacteria:   EBESCT00000029075
- EnsemblBacteria:   EBESCT00000056891
- GeneID:   914282
- GeneID:   960048
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5675
- KEGG:   ecs:ECs5058
- GeneTree:   EBGT00050000010655
- HOGENOM:   HBG416859
- OMA:   PDILEIF
- ProtClustDB:   PRK10370
- BioCyc:   ECOL83334:ECS5058-MONOMER
- GO:   GO:0005488
- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734
- Gene3D:   G3DSA:1.25.40.10

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22800; Mature: 22800

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50005 TPR; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRDPLHQFASQQNPEAQLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQ
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANYAQAIELWQKVMDLNSPRINR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TQLVESINMAKLLQRRSD
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRDPLHQFASQQNPEAQLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQLRGENAELYAALATVLYYQ
HHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANYAQAIELWQKVMDLNSPRINR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TQLVESINMAKLLQRRSD
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796