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The map label for this gene is zraS

Identifier: 209397343

GI number: 209397343

Start: 5113864

End: 5115240

Strand: Direct

Name: zraS

Synonym: ECH74115_5473

Alternate gene names: 209397343

Gene position: 5113864-5115240 (Clockwise)

Preceding gene: 209396958

Following gene: 209396243

Centisome position: 91.78

GC content: 54.61

Gene sequence:

>1377_bases
ATGCGTTTTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGT
AGGGTTGTTTGCGGTGACGGTGATTCGCGATTATGGCCGCGAGACTGCCGCCGCCAGACAAACGCTGCTGGAAAAAGGCA
GTGTACTTATTCGTGCTCTCGAATCCGGCTCGCGCGTCGGCATGGGGATGCGCATGCATCATGCGCAGCAGCAGGCCTTA
CTGGAAGAAATGGCCGGGCAGCCTGGAGTACGTTGGTTTGCGGTCACGGATGAACAAGGAACAATCGTGATGCATAGCAA
CTCCGGCATGGTGGGAAAACAGCTTTATTCCCCGCAGGAAATGCAGCAGTTACATCCGGGAGATGAAGAAGTGTGGCGGC
GGATCGATAGCGCAGACGGCGAGCCTGTTCTGGAAATTTATCGCCAGTTTCAACCGATGTTTGCTGCTGGAATGCACCGG
ATGCGCCATATGCAGCAGTATGCCGCGACACCACAAGCAATTTTCATCGCTTTCGACGCCAGTAATATTGTGAGTGCCGA
AGATCGTGAGCAGAGAAACACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCT
GGTATCGCCGCTATCTGCGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCAT
CTTGCGGCAGGCGTTGCCCACGAAATTCGTAACCCGCTTTCCTCGATTAAAGGGCTGGCAAAATACTTTGCCGAGCGCGC
GCCAGCAGGGGGAGAAGCGCATCAACTGGCGCAGGTGATGGCGAAAGAAGCTGACCGTTTAAACCGCGTGGTAAGCGAGC
TGCTGGAACTGGTTAAGCCAACGCATCTGGCTTTGCAGGCGGTGGATCTCAACACGCTGATTAACCACTCATTACAGCTG
GTAAGCCAGGATGCAAACTGCCGGGAGATCCAGTTACGCTTTACCGCCAACGACACGTTGCCGGAAATTCAGGCCGATCC
GGACAGGCTGACTCAGGTCCTGTTGAATCTTTATCTCAATGCTATTCAGGCGATTGGTCAGCATGGCGTGATTAGCGTGA
CGGCCAGCGAAAGCGGCGCGGGCGTGAAAATCAGCGTTACCGACAGCGGTAAGGGAATTGCGGCAGATCAGCTTGAAGCC
ATCTTCACCCCGTACTTCACCACCAAAGCCGAAGGCACCGGACTGGGGCTGGCGGTCGTGCATAATATTGTTGAACAACA
CGGTGGTACAATTCAGGTCGCAAGCCTGGAGGGAAAAGGCGCAAGGTTCACCCTCTGGCTTCCGGTCAATATTACGCGTA
AGGACCCACAAGGATGA

Upstream 100 bases:

>100_bases
TCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGG
ATGAGCAGGCAAAGAAGAAG

Downstream 100 bases:

>100_bases
CGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTGCTGCGCGGCTGG
GGCTATAACGTCGCGCTGGC

Product: sensor protein ZraS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQL
VSQDANCREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVNITRKDPQG

Sequences:

>Translated_458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQL
VSQDANCREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVNITRKDPQG
>Mature_458_residues
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQAL
LEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQL
VSQDANCREIQLRFTANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEA
IFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVNITRKDPQG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1790436, Length=465, Percent_Identity=84.9462365591398, Blast_Score=789, Evalue=0.0,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=42.5619834710744, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790300, Length=241, Percent_Identity=30.2904564315353, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994928, Length=245, Percent_Identity=29.3877551020408, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=26.9911504424779, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789808, Length=218, Percent_Identity=26.605504587156, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=27.2340425531915, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=218, Percent_Identity=28.8990825688073, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=29.4642857142857, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=26.4840182648402, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788393, Length=225, Percent_Identity=27.5555555555556, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=26.3636363636364, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=28.5067873303167, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082128, Length=225, Percent_Identity=24.4444444444444, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=226, Percent_Identity=30.5309734513274, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789149, Length=225, Percent_Identity=23.1111111111111, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790861, Length=211, Percent_Identity=27.4881516587678, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790567, Length=235, Percent_Identity=29.7872340425532, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ZRAS_ECO57 (Q8X614)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D86092
- PIR:   F91244
- RefSeq:   NP_290635.1
- RefSeq:   NP_312953.1
- ProteinModelPortal:   Q8X614
- SMR:   Q8X614
- EnsemblBacteria:   EBESCT00000026881
- EnsemblBacteria:   EBESCT00000059793
- GeneID:   916224
- GeneID:   960126
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5579
- KEGG:   ecs:ECs4926
- GeneTree:   EBGT00050000008663
- HOGENOM:   HBG467467
- OMA:   DQAFEYK
- ProtClustDB:   PRK10364
- BioCyc:   ECOL83334:ECS4926-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 50558; Mature: 50558

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1368e514)-; HASH(0x136115e4)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHH
ESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQE
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHCCHHH
MQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHRMRHMQQYAATPQAIFIAFDA
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
SNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
CCCCCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
THLALQAVDLNTLINHSLQLVSQDANCREIQLRFTANDTLPEIQADPDRLTQVLLNLYLN
HHHHHEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
AIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEAIFTPYFTTKAEGTGLGLAVV
HHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHH
HNIVEQHGGTIQVASLEGKGARFTLWLPVNITRKDPQG
HHHHHHCCCEEEEEEECCCCCEEEEEEEEEEECCCCCH
>Mature Secondary Structure
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAAARQTLLEKGSVLIRAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHH
ESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTIVMHSNSGMVGKQLYSPQE
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCHHHHCCHHH
MQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHRMRHMQQYAATPQAIFIAFDA
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
SNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKEKLVALGH
CCCCCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSELLELVKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
THLALQAVDLNTLINHSLQLVSQDANCREIQLRFTANDTLPEIQADPDRLTQVLLNLYLN
HHHHHEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
AIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLEAIFTPYFTTKAEGTGLGLAVV
HHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCEECCCCCCCCHHHHH
HNIVEQHGGTIQVASLEGKGARFTLWLPVNITRKDPQG
HHHHHHCCCEEEEEEECCCCCEEEEEEEEEEECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796