Click here to switch to the map view.

The map label for this gene is zraR

Identifier: 209396243

GI number: 209396243

Start: 5115237

End: 5116562

Strand: Direct

Name: zraR

Synonym: ECH74115_5475

Alternate gene names: 209396243

Gene position: 5115237-5116562 (Clockwise)

Preceding gene: 209397343

Following gene: 209400154

Centisome position: 91.8

GC content: 56.26

Gene sequence:

>1326_bases
ATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTGCTGCGCGG
CTGGGGCTATAACGTCGCGCTGGCGAACAGCGGGCGACAGGCGCTGGAGCAGGTGCGGGAGCGGGTTTTTGATCTTGTGC
TTTGCGATGTGCGAATGGCGGAGATGGACGGCATTGCCACGCTAAAAGAGATCAAAGCGTTAAATCCGGCGATTCCGGTA
CTGATTATGACCGCATACTCTAGCGTCGAGACGGCGGTAGAGGCGTTGAAAACCGGGGCGCTGGATTATCTCATCAAGCC
GCTGGATTTCGATAACCTGCAGGCGACGCTGGAAAAGGCGCTCGCGCATACGCACATTATTGATGCTGAAACGCCTGCGG
TGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA
TCGGAAGCCACGGTGCTGATCCACGGCGATTCCGGCACCGGTAAAGAGCTGGTCGCCAGGGCGATTCACGCCAGTAGCGC
ACGTAGCGAAAAACCGCTGGTAACGCTCAACTGTGCGGCACTCAACGAATCCTTGCTGGAATCTGAATTGTTCGGTCACG
AAAAAGGGGCGTTTACTGGAGCCGATAAACGCCGGGAAGGGCGCTTTGTTGAGGCGGACGGCGGCACGTTGTTTCTCGAT
GAAATTGGCGATATCTCGCCGATGATGCTGGTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAG
CAACCAGACTATTTCGGTTGATGTCCGGCTGATTGCCGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTT
TTCGCCAGGATCTCTACTATCGCCTGAACGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATTCCT
CTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCTGAACGTAATCGCAAGGCGGTAAAAGGCTTTACGCCCCAGGCGATGGA
TCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAACTGGAAAATGCGGTGGAACGGGCGGTGGTGCTGCTGACTG
GGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGCACAAAGCCTGGATATTCAG
CCGCTGGTGGAAGTCGAAAAAGAAGTGATTCTGGCGGCACTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCA
GTTAGGGATCACGCGCAAAACGCTGTTGGCAAAATTGTCGCGTTAG

Upstream 100 bases:

>100_bases
AACAACACGGTGGTACAATTCAGGTCGCAAGCCTGGAGGGAAAAGGCGCAAGGTTCACCCTCTGGCTTCCGGTCAATATT
ACGCGTAAGGACCCACAAGG

Downstream 100 bases:

>100_bases
TTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGC
ATAGGCGCGTTTCTGTGCTT

Product: transcriptional regulatory protein ZraR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPV
LIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIP
LLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLAQSLDIQ
PLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR

Sequences:

>Translated_441_residues
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPV
LIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIP
LLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLAQSLDIQ
PLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR
>Mature_440_residues
THDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVL
IMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPS
EATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDE
IGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPL
LAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLAQSLDIQP
LVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=98.6394557823129, Blast_Score=881, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=446, Percent_Identity=48.4304932735426, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1788905, Length=448, Percent_Identity=44.4196428571429, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI1790299, Length=462, Percent_Identity=42.2077922077922, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI87082117, Length=315, Percent_Identity=47.9365079365079, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1789087, Length=311, Percent_Identity=47.2668810289389, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=47.3684210526316, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789233, Length=316, Percent_Identity=43.6708860759494, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=43.3544303797468, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=43.558282208589, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1787583, Length=305, Percent_Identity=36.3934426229508, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789828, Length=256, Percent_Identity=39.0625, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081858, Length=320, Percent_Identity=31.875, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789149, Length=112, Percent_Identity=33.9285714285714, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789402, Length=118, Percent_Identity=33.0508474576271, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=131, Percent_Identity=31.2977099236641, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=34.4262295081967, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI145693157, Length=115, Percent_Identity=34.7826086956522, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ZRAR_ECO57 (Q8X613)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E86092
- PIR:   G91244
- RefSeq:   NP_290636.1
- RefSeq:   NP_312954.1
- ProteinModelPortal:   Q8X613
- SMR:   Q8X613
- EnsemblBacteria:   EBESCT00000026898
- EnsemblBacteria:   EBESCT00000057525
- GeneID:   916250
- GeneID:   960125
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5580
- KEGG:   ecs:ECs4927
- GeneTree:   EBGT00050000008655
- HOGENOM:   HBG740884
- OMA:   GKSSAMQ
- ProtClustDB:   PRK10365
- BioCyc:   ECOL83334:ECS4927-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00448

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat; SSF52172 CheY_like; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 48421; Mature: 48290

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50110 RESPONSE_REGULATORY; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMA
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKA
HHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
LAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVAR
HHHHHEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
AIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHE
RLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAVERAVVLLTGEYISERELPLAIASTPIPLAQSLDIQPLVEVEKEVILAALEKTGGN
HHHHHHHEEEEEECCHHCCCCCCEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCC
KTEAARQLGITRKTLLAKLSR
HHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
THDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVRERVFDLVLCDVRMA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALDYLIKPLDFDNLQATLEKA
HHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
LAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVAR
HHHHHEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
AIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHE
RLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAVERAVVLLTGEYISERELPLAIASTPIPLAQSLDIQPLVEVEKEVILAALEKTGGN
HHHHHHHEEEEEECCHHCCCCCCEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCC
KTEAARQLGITRKTLLAKLSR
HHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796