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The map label for this gene is ydeO [C]

Identifier: 209396156

GI number: 209396156

Start: 2018773

End: 2019534

Strand: Reverse

Name: ydeO [C]

Synonym: ECH74115_2112

Alternate gene names: 209396156

Gene position: 2019534-2018773 (Counterclockwise)

Preceding gene: 209400612

Following gene: 209397007

Centisome position: 36.24

GC content: 39.24

Gene sequence:

>762_bases
ATGTCGCTCGTTTGTTCTGTTATATTTATTCATCATGCCTTCAACGCTAACATTTTAGATAAAGATTACGCCTTCTCTGA
CGGCGAGATCCTGATGGTAGATAACGCTGTTCGTACGCATTTTGAACCTTATGAGCGGCATTTTAAAGAAATCGGATTTA
ATGAAAATACCATTAAAAAATATCTACAATGCACTAACATCCAGACAGTGACGATGCCTGTTCCTGCGAAGTTTTTACGT
GCTTCGAATGTACCGACTGGATTGCTTAATGAAATGATTGCTTATCTCAACTCGGAAGAACGCAATCATCATAATTTTTC
AGAACTTTTGCTTTTTTCTTGCCTGTCTATTTTTGCCACATGCAAAGGTTTCATTACACTATTAACTAACGGTGTGCTAT
CCGTTTCGGGGAAAGTGAGAAATATTGTCAACATGAAGCTGGCGCACCCATGGAAGCTGAAAGATATTTGTGACTGCCTG
TACATCAGTGAAAGCCTGTTGAAGAAAAAACTAAAGCAAGAGCAAACGACATTCTCACAGATTCTTTTAGATGCCAGAAT
GCAGCACGCAAAAAATTTGATACGCGTAGAAGGTTCAGTCAATAAAATTGCCGAACAATGTGGTTATGCCAGTACATCTT
ATTTTATTTATGCGTTCCGCAAACATTTCGGCAACAGTCCAAAGAGAGTTTCTAAGGAGTACCGTTGTCAAAGGCACACG
GGTATGAATACGGGCAACACGATGAATGCTTTAGCTATTTGA

Upstream 100 bases:

>100_bases
TTCATTGTCAATAGTTTGCAAGACTGAAAATAAACAGCAAATTATAAATATGAAATGTTAAAAAAGTATCGATAAAACTT
TATTGTTTTAAGGAGATAAA

Downstream 100 bases:

>100_bases
TTATTTGCTAACGAGTAGTCAACCACGCACCCTACGTAAAAATTAAAAATGGGGCAGTCATTCCCTGCCCCGCGTTGTTT
TTAGGCGATATATTTATTGA

Product: transcriptional regulator YdeO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLR
ASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHT
GMNTGNTMNALAI

Sequences:

>Translated_253_residues
MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLR
ASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHT
GMNTGNTMNALAI
>Mature_252_residues
SLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRA
SNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLY
ISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTG
MNTGNTMNALAI

Specific function: Induces the expression of gadE and mdtEF. Could also regulate the expression of other genes involved in acid resistance

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787776, Length=253, Percent_Identity=98.0237154150198, Blast_Score=519, Evalue=1e-149,
Organism=Escherichia coli, GI1789932, Length=237, Percent_Identity=34.1772151898734, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1786776, Length=235, Percent_Identity=28.936170212766, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789933, Length=144, Percent_Identity=40.9722222222222, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790557, Length=144, Percent_Identity=34.7222222222222, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786778, Length=124, Percent_Identity=38.7096774193548, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YDEO_ECO57 (Q8X4Z9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85726
- PIR:   H90891
- RefSeq:   NP_287656.1
- RefSeq:   NP_310131.1
- ProteinModelPortal:   Q8X4Z9
- EnsemblBacteria:   EBESCT00000027566
- EnsemblBacteria:   EBESCT00000057478
- GeneID:   917312
- GeneID:   960668
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2209
- KEGG:   ecs:ECs2104
- GeneTree:   EBGT00050000008641
- HOGENOM:   HBG467005
- OMA:   HMGMNTG
- ProtClustDB:   PRK09940
- BioCyc:   ECOL83334:ECS2104-MONOMER
- GO:   GO:0005622
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- Gene3D:   G3DSA:1.10.10.60
- PRINTS:   PR00032
- SMART:   SM00342

Pfam domain/function: PF00165 HTH_AraC; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 28886; Mature: 28755

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKK
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHCCCHHHHHHHHCCCCHHHHHH
YLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAT
HHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
CKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQ
HHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHT
HHHHHHHHHHHHHEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GMNTGNTMNALAI
CCCCCCCCHHHCC
>Mature Secondary Structure 
SLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHCCCHHHHHHHHCCCCHHHHHH
YLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAT
HHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
CKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQ
HHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHT
HHHHHHHHHHHHHEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GMNTGNTMNALAI
CCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796