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The map label for this gene is ydeM [H]

Identifier: 209397007

GI number: 209397007

Start: 2015478

End: 2016635

Strand: Reverse

Name: ydeM [H]

Synonym: ECH74115_2110

Alternate gene names: 209397007

Gene position: 2016635-2015478 (Counterclockwise)

Preceding gene: 209396156

Following gene: 209399273

Centisome position: 36.19

GC content: 39.03

Gene sequence:

>1158_bases
ATGCACGTTACAGCCAAGCCCTCCAGTTTTCAATGTAATCTCAAATGTGATTACTGTTTTTACCTTGAAAAAGAGTCGCA
GTTTACTCATGAAAAATGGATGGATGACAGCACTCTGAAAGAGTTCATCAAACAATATATCGCAGCGTCTGGCAATCAGG
TCTATTTTACCTGGCAAGGCGGTGAACCCACTCTGGCTGGCCTGGATTTTTTCCGTAAAGTTATTCACTATCAACAACGC
TATGCAGGCCAAAAACGTATTTTTAATGCATTACAAACGAATGGCATTTTATTGAATAATGAATGGTGTGCCTTTCTCAA
AGAACATGAGTTTCTGGTTGGTATCTCGATCGATGGACCCCAGGAGCTACATGACCGTTACAGACGCAGTAATTCAGGTA
ACGGTACTTTTGCAAAAGTGATAGCAGCCATCGAGCGTCTGAAATCATATCAAGTAGAGTTTAATACGTTAACCGTCATT
AATAACGTTAATGTCCATTACCCTCTTGAGGTTTATCATTTTTTAAAATCTATCGGCAGTAAACATATGCAATTTATCGA
ATTGCTCGAAACCGGGACGCCGAATATTGATTTCAGTGGTCATAGTGAGAACACATTCCGTATCATTGATTTTTCTGTGC
CTCCCACGGCTTATGGCAAGTTTATGTCAACCATTTTTATGCAATGGGTTAAAAACGATGTGGGTGAAATTTTCATCCGT
CAGTTTGAAAGCTTTGTCAGCCGTTTTTTGGGGAATGGGCATACCAGTTGTATTTTCCAGGAGTCCTGCAAGGATAATCT
GGTTGTTGAAAGTAATGGAGATATTTACGAATGCGATCATTTTGTCTATCCACAGTACAAACTTGGAAACATTAATAAAT
CTGAACTTAAAACGATGAACAGTGTACAACTGACAGCGCAAAAAAAACGGATTTCAGCGAAATGTCAGCAATGTGCATAT
AAACCTATCTGCAATGGCGGTTGCCCTAAGCATCGTATTACTAAAGTAAACGATGAGACTGTTTCCTATTTTTGCGAAGG
TTATAAAATCCTTTTTTCAACCATGGTACCTTATATGAACGCCATGGTGGAGTTAGCCAAGAACAGAGTACCGCTTTACC
ACATTATGGATGTTGCAAAACAAATGGAGAATAATTAA

Upstream 100 bases:

>100_bases
TCAGGAGAAGTTTAACAATATCAAGAAAGCACTAAGCGAAGCGAAATAACTAAACCTTCATGCGGCGGATTTTTCCGCCG
CCTTATTGAGCGAGATAGCG

Downstream 100 bases:

>100_bases
TAACGCTCTCTTTTAGCTCCATTCTTAGCCTCCTTCTTATGACCTTAACAAGGCACCCGACATAAATTCCTGTCGAGTGT
TTCGTCATCATCATCTTCCC

Product: radical SAM domain protein

Products: NA

Alternate protein names: AnSME homolog [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQR
YAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAY
KPICNGGCPKHRITKVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN

Sequences:

>Translated_385_residues
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQR
YAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAY
KPICNGGCPKHRITKVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN
>Mature_385_residues
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQR
YAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAY
KPICNGGCPKHRITKVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN

Specific function: Unknown

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=385, Percent_Identity=99.2207792207792, Blast_Score=806, Evalue=0.0,
Organism=Escherichia coli, GI48994973, Length=392, Percent_Identity=41.3265306122449, Blast_Score=332, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 44509; Mature: 44509

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQG
CCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEC
GEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGP
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEECCHHHHHHCCCCEEEEEECCCH
QELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVINNVNVHYPLEVYHFLKSIGS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEEECCCEECHHHHHHHHHHHHH
KHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKLGNINKSELKTMN
HHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCEEECCCEECCCEEECCCCHHHHHHHH
SVQLTAQKKRISAKCQQCAYKPICNGGCPKHRITKVNDETVSYFCEGYKILFSTMVPYMN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
AMVELAKNRVPLYHIMDVAKQMENN
HHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIKQYIAASGNQVYFTWQG
CCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEC
GEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEWCAFLKEHEFLVGISIDGP
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEECCHHHHHHCCCCEEEEEECCCH
QELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVINNVNVHYPLEVYHFLKSIGS
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEEECCCEECHHHHHHHHHHHHH
KHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFMQWVKNDVGEIFIR
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQYKLGNINKSELKTMN
HHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCEEECCCEECCCEEECCCCHHHHHHHH
SVQLTAQKKRISAKCQQCAYKPICNGGCPKHRITKVNDETVSYFCEGYKILFSTMVPYMN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHH
AMVELAKNRVPLYHIMDVAKQMENN
HHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]