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The map label for this gene is Crp2 [H]

Identifier: 20809102

GI number: 20809102

Start: 2664838

End: 2665515

Strand: Reverse

Name: Crp2 [H]

Synonym: TTE2773

Alternate gene names: 20809102

Gene position: 2665515-2664838 (Counterclockwise)

Preceding gene: 20809103

Following gene: 161760530

Centisome position: 99.11

GC content: 34.22

Gene sequence:

>678_bases
GTGAAAGAACTTAAATATTTAAGAAAAATCCCTTACTTCAGTGAGCTAGAAGATGAAAAGCTCGAGAAACTACACAACAT
TGCAACATTAAAACCTGTCAAAAAGGGATGCATTATCTTCACAGAAGGTCAAAAAGGAGAAGCTATATATTTTGTGAAGA
CAGGAAAAATAAAAATCTCCAAAATCTCTTCTGTCGGGAAAGAATATACTATAAAGATAATGGAAGAAGGGGATGTCTTC
GGAGAATCTACCTTATTTATAGGAGGCGAATATCCGGCTACTGCTGAAGCAATAGAGGATAGCGAAATTTTGGAACTAAG
AAATAAAGACATAGAAGACCTAATTTTAAAAGATACCCAAATAGCCCTTAGCATAATAAAGATATTGGCAAAAAGGTTGA
AATACATAGCTGTTGTGATAGAAAACCTAGCTTTAAGAGACTCTGTAGGAAGAACAGCTTCTATTTTACTTACTTTTGCG
AGAGAAAGGGGCATATCCACAAAAGAAGGAATTTTGGTAGAAATAGACCTAAAAAGACAGGAACTGGCCAATTTAGCCGG
AACTTCTCGAGAAAATATCACAAGGATTTTAAGTCAAATGGATAGAGATGGAATAATAAAGCTTGGCAAAGACAAAATAT
TGATAAAAGATTTAGAAGAACTCAGAAAAATGCTCTGA

Upstream 100 bases:

>100_bases
ACCTACAGGAGTGGTAGAGCTTTTGTGGCTCGCTCAGTATACAAAATTTGTAAGTTTAGATAAGTACAGGACAGAAGAAG
GATAAAGGGTGGTAAAGCCC

Downstream 100 bases:

>100_bases
TTTTGTCCCAAAGTTATTTGGGACTTTTTTATTTTATGTGATTTTTGTCACAGATTTTTCTCTTAATATACCCTATCATT
TACTATGTAAAAACAAATTT

Product: cAMP-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKELKYLRKIPYFSELEDEKLEKLHNIATLKPVKKGCIIFTEGQKGEAIYFVKTGKIKISKISSVGKEYTIKIMEEGDVF
GESTLFIGGEYPATAEAIEDSEILELRNKDIEDLILKDTQIALSIIKILAKRLKYIAVVIENLALRDSVGRTASILLTFA
RERGISTKEGILVEIDLKRQELANLAGTSRENITRILSQMDRDGIIKLGKDKILIKDLEELRKML

Sequences:

>Translated_225_residues
MKELKYLRKIPYFSELEDEKLEKLHNIATLKPVKKGCIIFTEGQKGEAIYFVKTGKIKISKISSVGKEYTIKIMEEGDVF
GESTLFIGGEYPATAEAIEDSEILELRNKDIEDLILKDTQIALSIIKILAKRLKYIAVVIENLALRDSVGRTASILLTFA
RERGISTKEGILVEIDLKRQELANLAGTSRENITRILSQMDRDGIIKLGKDKILIKDLEELRKML
>Mature_225_residues
MKELKYLRKIPYFSELEDEKLEKLHNIATLKPVKKGCIIFTEGQKGEAIYFVKTGKIKISKISSVGKEYTIKIMEEGDVF
GESTLFIGGEYPATAEAIEDSEILELRNKDIEDLILKDTQIALSIIKILAKRLKYIAVVIENLALRDSVGRTASILLTFA
RERGISTKEGILVEIDLKRQELANLAGTSRENITRILSQMDRDGIIKLGKDKILIKDLEELRKML

Specific function: It is involved in the activation of genes necessary for anaerobic respiration [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=200, Percent_Identity=27, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787595, Length=219, Percent_Identity=24.6575342465753, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25471; Mature: 25471

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELKYLRKIPYFSELEDEKLEKLHNIATLKPVKKGCIIFTEGQKGEAIYFVKTGKIKIS
CCHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCEEEE
KISSVGKEYTIKIMEEGDVFGESTLFIGGEYPATAEAIEDSEILELRNKDIEDLILKDTQ
HHHHCCCEEEEEEEECCCCCCCCEEEECCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHH
IALSIIKILAKRLKYIAVVIENLALRDSVGRTASILLTFARERGISTKEGILVEIDLKRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHH
ELANLAGTSRENITRILSQMDRDGIIKLGKDKILIKDLEELRKML
HHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MKELKYLRKIPYFSELEDEKLEKLHNIATLKPVKKGCIIFTEGQKGEAIYFVKTGKIKIS
CCHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCEEEE
KISSVGKEYTIKIMEEGDVFGESTLFIGGEYPATAEAIEDSEILELRNKDIEDLILKDTQ
HHHHCCCEEEEEEEECCCCCCCCEEEECCCCCCHHHHCCCHHHHHHHCCCHHHHHHHHHH
IALSIIKILAKRLKYIAVVIENLALRDSVGRTASILLTFARERGISTKEGILVEIDLKRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHH
ELANLAGTSRENITRILSQMDRDGIIKLGKDKILIKDLEELRKML
HHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377 [H]