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The map label for this gene is DnaB [H]

Identifier: 20809103

GI number: 20809103

Start: 2665531

End: 2666865

Strand: Reverse

Name: DnaB [H]

Synonym: TTE2774

Alternate gene names: 20809103

Gene position: 2666865-2665531 (Counterclockwise)

Preceding gene: 20809104

Following gene: 20809102

Centisome position: 99.16

GC content: 40.37

Gene sequence:

>1335_bases
ATGGACAGGACGCGCATCCCTCCCCAAAACATAGAGGCAGAACAATCGGTTTTAGGTTCAATGCTCTTGTCCAGAGAAGC
CATAATAGAAGTGTCTGAGATATTAAGAGCAGAGGATTTTTATAAAGAATCCCACAAGAAACTTTTTGACGTAATAATGG
AAATGTTTGAAAAGGACATACCAGTCGACCTTGTTACAGTAGTGGATGAGCTAAGGAAAAGGGACATGCTGGAAGTAGTG
GGCGGAATAGAATACCTTGCAAGCCTCACTTCCAGCGTAATTACCACAGCCAATGTCTCCTATTACGCTAAACTTATAAA
AGAAAAAGCTACTTTAAGGCGGCTTATTGAGGCATCTTCAGAGATTATGGAATTAAGCTATCAGGAAGACGACGTGGAAA
CTGTTTTAGATATTGCCGAACAGAAAATATTCGACATAGCCCAGGGAAGGAATACCACTACTTTTTCTTCAATGAAAGAC
ATTTTAATGGATACTTTTTATAAAATTGAAGAGCTTTATAAAAACAAAGGAAAGCTTACAGGTATACCTTCGGGTTTTCC
AGATTTAGATGCCAAGACAGCGGGATTTCAGCCTTCTGACTTTATATTAATCGCTGCAAGGCCTTCAATGGGCAAGACTT
CATTTGCGCTAAACATTGCACAAAATGCCGCACTTTTAACAGGGCTTCCTGTTGCTATATTTAGTTTAGAGATGTCAAAA
GAACAGCTTGTCACAAGGCTTATATGCTCAACGGCCAATATTGACAGCCAGAAACTCAGGACCGGAAATTTAGATGAAGA
GGACTGGATGAAATTAGCAGCTGCCATGACTCCTCTTTCAAAGGCTCCTATCTACATTGACGATACTCCCGGTGTAACTG
TAATGGACATAAGAGCAAAAGCAAGGAGATTAAAGCTTGAAAAGGGCTTAGGGTTGGTGATGATAGATTACCTTCAGCTT
ATGCAGGGAAGAGGAAGAGCAGAAAACAGACAGCAAGAAATTTCAGAAATATCAAGGTCATTAAAAAGCCTGGCAAGAGA
ATTAAACGTCCCAGTAATCACCTTATCCCAGCTTTCACGCGCACCTGAGACCAGGTCAGACCACAGGCCTGTTTTAAGCG
ATTTGAGAGAATCAGGAGCCATAGAGCAGGATGCTGACATTGTCATGTTTTTGTATAGAGATGATTACTATCATAAAGAC
TCTGAAAAGAAAAACATAGCAGAAGTGATAATTGCAAAACACAGAAACGGACCTACAGGAGTGGTAGAGCTTTTGTGGCT
CGCTCAGTATACAAAATTTGTAAGTTTAGATAAGTACAGGACAGAAGAAGGATAA

Upstream 100 bases:

>100_bases
ACAACTGGCACTTACTATGCCGAGATAAAATTGTATCAGGGAGTTACAGCAAAAGTGCAGGTGGATGTGGTAGCCGAGTG
AAAAGTGAGGGGGAATATGA

Downstream 100 bases:

>100_bases
AGGGTGGTAAAGCCCGTGAAAGAACTTAAATATTTAAGAAAAATCCCTTACTTCAGTGAGCTAGAAGATGAAAAGCTCGA
GAAACTACACAACATTGCAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MDRTRIPPQNIEAEQSVLGSMLLSREAIIEVSEILRAEDFYKESHKKLFDVIMEMFEKDIPVDLVTVVDELRKRDMLEVV
GGIEYLASLTSSVITTANVSYYAKLIKEKATLRRLIEASSEIMELSYQEDDVETVLDIAEQKIFDIAQGRNTTTFSSMKD
ILMDTFYKIEELYKNKGKLTGIPSGFPDLDAKTAGFQPSDFILIAARPSMGKTSFALNIAQNAALLTGLPVAIFSLEMSK
EQLVTRLICSTANIDSQKLRTGNLDEEDWMKLAAAMTPLSKAPIYIDDTPGVTVMDIRAKARRLKLEKGLGLVMIDYLQL
MQGRGRAENRQQEISEISRSLKSLARELNVPVITLSQLSRAPETRSDHRPVLSDLRESGAIEQDADIVMFLYRDDYYHKD
SEKKNIAEVIIAKHRNGPTGVVELLWLAQYTKFVSLDKYRTEEG

Sequences:

>Translated_444_residues
MDRTRIPPQNIEAEQSVLGSMLLSREAIIEVSEILRAEDFYKESHKKLFDVIMEMFEKDIPVDLVTVVDELRKRDMLEVV
GGIEYLASLTSSVITTANVSYYAKLIKEKATLRRLIEASSEIMELSYQEDDVETVLDIAEQKIFDIAQGRNTTTFSSMKD
ILMDTFYKIEELYKNKGKLTGIPSGFPDLDAKTAGFQPSDFILIAARPSMGKTSFALNIAQNAALLTGLPVAIFSLEMSK
EQLVTRLICSTANIDSQKLRTGNLDEEDWMKLAAAMTPLSKAPIYIDDTPGVTVMDIRAKARRLKLEKGLGLVMIDYLQL
MQGRGRAENRQQEISEISRSLKSLARELNVPVITLSQLSRAPETRSDHRPVLSDLRESGAIEQDADIVMFLYRDDYYHKD
SEKKNIAEVIIAKHRNGPTGVVELLWLAQYTKFVSLDKYRTEEG
>Mature_444_residues
MDRTRIPPQNIEAEQSVLGSMLLSREAIIEVSEILRAEDFYKESHKKLFDVIMEMFEKDIPVDLVTVVDELRKRDMLEVV
GGIEYLASLTSSVITTANVSYYAKLIKEKATLRRLIEASSEIMELSYQEDDVETVLDIAEQKIFDIAQGRNTTTFSSMKD
ILMDTFYKIEELYKNKGKLTGIPSGFPDLDAKTAGFQPSDFILIAARPSMGKTSFALNIAQNAALLTGLPVAIFSLEMSK
EQLVTRLICSTANIDSQKLRTGNLDEEDWMKLAAAMTPLSKAPIYIDDTPGVTVMDIRAKARRLKLEKGLGLVMIDYLQL
MQGRGRAENRQQEISEISRSLKSLARELNVPVITLSQLSRAPETRSDHRPVLSDLRESGAIEQDADIVMFLYRDDYYHKD
SEKKNIAEVIIAKHRNGPTGVVELLWLAQYTKFVSLDKYRTEEG

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=436, Percent_Identity=47.7064220183486, Blast_Score=420, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50095; Mature: 50095

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRTRIPPQNIEAEQSVLGSMLLSREAIIEVSEILRAEDFYKESHKKLFDVIMEMFEKDI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVDLVTVVDELRKRDMLEVVGGIEYLASLTSSVITTANVSYYAKLIKEKATLRRLIEASS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIMELSYQEDDVETVLDIAEQKIFDIAQGRNTTTFSSMKDILMDTFYKIEELYKNKGKLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GIPSGFPDLDAKTAGFQPSDFILIAARPSMGKTSFALNIAQNAALLTGLPVAIFSLEMSK
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEHHHCHHHHHCCHHHHHHHHCCH
EQLVTRLICSTANIDSQKLRTGNLDEEDWMKLAAAMTPLSKAPIYIDDTPGVTVMDIRAK
HHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEHHHH
ARRLKLEKGLGLVMIDYLQLMQGRGRAENRQQEISEISRSLKSLARELNVPVITLSQLSR
HHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHC
APETRSDHRPVLSDLRESGAIEQDADIVMFLYRDDYYHKDSEKKNIAEVIIAKHRNGPTG
CCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHH
VVELLWLAQYTKFVSLDKYRTEEG
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDRTRIPPQNIEAEQSVLGSMLLSREAIIEVSEILRAEDFYKESHKKLFDVIMEMFEKDI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVDLVTVVDELRKRDMLEVVGGIEYLASLTSSVITTANVSYYAKLIKEKATLRRLIEASS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIMELSYQEDDVETVLDIAEQKIFDIAQGRNTTTFSSMKDILMDTFYKIEELYKNKGKLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GIPSGFPDLDAKTAGFQPSDFILIAARPSMGKTSFALNIAQNAALLTGLPVAIFSLEMSK
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEHHHCHHHHHCCHHHHHHHHCCH
EQLVTRLICSTANIDSQKLRTGNLDEEDWMKLAAAMTPLSKAPIYIDDTPGVTVMDIRAK
HHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEHHHH
ARRLKLEKGLGLVMIDYLQLMQGRGRAENRQQEISEISRSLKSLARELNVPVITLSQLSR
HHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHC
APETRSDHRPVLSDLRESGAIEQDADIVMFLYRDDYYHKDSEKKNIAEVIIAKHRNGPTG
CCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHH
VVELLWLAQYTKFVSLDKYRTEEG
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]