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The map label for this gene is MurF [H]

Identifier: 20808896

GI number: 20808896

Start: 2435938

End: 2437464

Strand: Reverse

Name: MurF [H]

Synonym: TTE2546

Alternate gene names: 20808896

Gene position: 2437464-2435938 (Counterclockwise)

Preceding gene: 20808897

Following gene: 20808895

Centisome position: 90.63

GC content: 38.83

Gene sequence:

>1527_bases
ATGGCACAGCTGGAAGGCTACAAGATAAAAAATTTTTTCAAGTGGCATTTTAGAAATTATGACAGGTGGGTAAAAGAGTC
ATTGCTTGCACTTTTGAGTGCGGGTATCGCCTTTATTATGAATATTTTTAGACTTCCTTCAGAAATTATCATTTTAGTAC
CTATAGCGCTGTTTTTTTATATAACTTACAAAGAAGTTACGACTAAAAAAAAGGCGAAAAAACCCTTGGTCTTTACTGCC
AGAGCGACAAGGCTTTTTTTGACTTTCTGGCTTCTTATAGCTTTATCCTTTTACTTTTTGAAGAAGAATTTTCAAGCTCC
AGAAGTTTTTTCTGCTGAGGTGCTAATTCTGACGGCTTTGTTGCCAGTTTATATGGCTTTGAGCTTAGTAATTACCTTCC
CCTTAGAAAAATTTATTCAGTTTTTATATTATAAATCAGCTCAGCGCAAGATAAGGAATAGAAAAGATTTAATTACAATA
GGTATAACTGGGAGCTACGGCAAAACAAGCACAAAGCATTTTATAACAGCTGTTCTTTCAGAAAAATTTTCTGTTTTAAT
GACTCCGGAAAGTTATAATACGCCTATGGGGGTCACAAAGGTCATCAGAAATGAGCTAAAAGATACCCATCAGATTTTTG
TTTGTGAAATGGGGGCGAGACAGAGGGGAGATATAAAAGTTCTAGTAAAGCTTGCGAATCCCAAGATAGGAGTGATTACA
TCAATAGGGCCTCAGCATTTGGAGACTTTTGGAACGATAGAGAATGTTGCTAAAACTAAGTTTGAACTGGTAGAAGGTTT
GCCAGAAGATGGGATTGCTGTACTAAATGGGGATAATTATTACTGCAGGACTTTTGGAGAAAAATTTGCTGTGCGAAAAT
TATTCTATGGCACAGAAAGTCCAATTGAAAGGAAATATTTTCTGTGGGCTGAGAATATCATTAATACTAGACAAGGCCTC
AAGTTTGACGCGGTGACTGAAGATGGGATGAGAATAAAATGTGAGGTACCACTGCTTGGAAGGCACAATGTGAGTAACTT
ATTGGCTGCGATTTCTGTGGCACTGGCATTAGGACTAAACGAGGAAGAGATAAAAAGAGGCCTTTCCAGAATTGAACCTG
TTCCCCACAGGCTTCAGCTTATATCCACTCCTCATGGCGTAACTGTTATTGACGACGCCTTTAACTCAAACCCTGAGGGG
GCAAAAGAGGCCCTTGCCTGTCTTAAGGAGATGGATGCAAAGAGGAGGATAATCGTAACTCCAGGAATGGTGGAACTGGG
GGAGAAAGAGTATGAAGAGAATAAAAATCTTGGGAAAAACATTGCAGAGGTCTGTGACTATGCAATTTTAGTCGGGAAAA
AGAGAAGTAGACCTATTTTTGAAGGGTTGAAGGAAGCCAGTTTTCCCGAAGAAAAAGTGTTTGTTGTGGCTAACTTGGAT
GAGGCTACAAAAGTTTTACAGACGCTACTTCAACCGGGAGATGTGGTGCTTTTTGAAAATGACTTGCCTGATAATTATGA
AGGATGA

Upstream 100 bases:

>100_bases
ATTTCTTGAGGTGATAAGAGTGGCAAATTTGATTGGGATAGCTCTTGTAGTTTTATCTTTTATCTTGGTTGTGTTTTTCT
ACGGAAAGAAGGAGCTTCAT

Downstream 100 bases:

>100_bases
GGAGGATTTTTATGTTGAAAGTGGCTGTGATGTTTGGAGGAAAATCAGTGGAGCACGAGGTTTCTATAATTACAGGGCTC
CAGGTCATTGAGAATATGAA

Product: UDP-N-acetylmuramyl pentapeptide synthase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MAQLEGYKIKNFFKWHFRNYDRWVKESLLALLSAGIAFIMNIFRLPSEIIILVPIALFFYITYKEVTTKKKAKKPLVFTA
RATRLFLTFWLLIALSFYFLKKNFQAPEVFSAEVLILTALLPVYMALSLVITFPLEKFIQFLYYKSAQRKIRNRKDLITI
GITGSYGKTSTKHFITAVLSEKFSVLMTPESYNTPMGVTKVIRNELKDTHQIFVCEMGARQRGDIKVLVKLANPKIGVIT
SIGPQHLETFGTIENVAKTKFELVEGLPEDGIAVLNGDNYYCRTFGEKFAVRKLFYGTESPIERKYFLWAENIINTRQGL
KFDAVTEDGMRIKCEVPLLGRHNVSNLLAAISVALALGLNEEEIKRGLSRIEPVPHRLQLISTPHGVTVIDDAFNSNPEG
AKEALACLKEMDAKRRIIVTPGMVELGEKEYEENKNLGKNIAEVCDYAILVGKKRSRPIFEGLKEASFPEEKVFVVANLD
EATKVLQTLLQPGDVVLFENDLPDNYEG

Sequences:

>Translated_508_residues
MAQLEGYKIKNFFKWHFRNYDRWVKESLLALLSAGIAFIMNIFRLPSEIIILVPIALFFYITYKEVTTKKKAKKPLVFTA
RATRLFLTFWLLIALSFYFLKKNFQAPEVFSAEVLILTALLPVYMALSLVITFPLEKFIQFLYYKSAQRKIRNRKDLITI
GITGSYGKTSTKHFITAVLSEKFSVLMTPESYNTPMGVTKVIRNELKDTHQIFVCEMGARQRGDIKVLVKLANPKIGVIT
SIGPQHLETFGTIENVAKTKFELVEGLPEDGIAVLNGDNYYCRTFGEKFAVRKLFYGTESPIERKYFLWAENIINTRQGL
KFDAVTEDGMRIKCEVPLLGRHNVSNLLAAISVALALGLNEEEIKRGLSRIEPVPHRLQLISTPHGVTVIDDAFNSNPEG
AKEALACLKEMDAKRRIIVTPGMVELGEKEYEENKNLGKNIAEVCDYAILVGKKRSRPIFEGLKEASFPEEKVFVVANLD
EATKVLQTLLQPGDVVLFENDLPDNYEG
>Mature_507_residues
AQLEGYKIKNFFKWHFRNYDRWVKESLLALLSAGIAFIMNIFRLPSEIIILVPIALFFYITYKEVTTKKKAKKPLVFTAR
ATRLFLTFWLLIALSFYFLKKNFQAPEVFSAEVLILTALLPVYMALSLVITFPLEKFIQFLYYKSAQRKIRNRKDLITIG
ITGSYGKTSTKHFITAVLSEKFSVLMTPESYNTPMGVTKVIRNELKDTHQIFVCEMGARQRGDIKVLVKLANPKIGVITS
IGPQHLETFGTIENVAKTKFELVEGLPEDGIAVLNGDNYYCRTFGEKFAVRKLFYGTESPIERKYFLWAENIINTRQGLK
FDAVTEDGMRIKCEVPLLGRHNVSNLLAAISVALALGLNEEEIKRGLSRIEPVPHRLQLISTPHGVTVIDDAFNSNPEGA
KEALACLKEMDAKRRIIVTPGMVELGEKEYEENKNLGKNIAEVCDYAILVGKKRSRPIFEGLKEASFPEEKVFVVANLDE
ATKVLQTLLQPGDVVLFENDLPDNYEG

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=337, Percent_Identity=31.1572700296736, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 57602; Mature: 57471

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLEGYKIKNFFKWHFRNYDRWVKESLLALLSAGIAFIMNIFRLPSEIIILVPIALFFY
CCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ITYKEVTTKKKAKKPLVFTARATRLFLTFWLLIALSFYFLKKNFQAPEVFSAEVLILTAL
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LPVYMALSLVITFPLEKFIQFLYYKSAQRKIRNRKDLITIGITGSYGKTSTKHFITAVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
EKFSVLMTPESYNTPMGVTKVIRNELKDTHQIFVCEMGARQRGDIKVLVKLANPKIGVIT
CCCEEEECCCCCCCCHHHHHHHHHHHCHHHEEEEEECCCCCCCCEEEEEEECCCCEEEEE
SIGPQHLETFGTIENVAKTKFELVEGLPEDGIAVLNGDNYYCRTFGEKFAVRKLFYGTES
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHCCCCC
PIERKYFLWAENIINTRQGLKFDAVTEDGMRIKCEVPLLGRHNVSNLLAAISVALALGLN
CCCHHHHEEHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCC
EEEIKRGLSRIEPVPHRLQLISTPHGVTVIDDAFNSNPEGAKEALACLKEMDAKRRIIVT
HHHHHHHHHHCCCCCCHHEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEC
PGMVELGEKEYEENKNLGKNIAEVCDYAILVGKKRSRPIFEGLKEASFPEEKVFVVANLD
CCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCH
EATKVLQTLLQPGDVVLFENDLPDNYEG
HHHHHHHHHHCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
AQLEGYKIKNFFKWHFRNYDRWVKESLLALLSAGIAFIMNIFRLPSEIIILVPIALFFY
CCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ITYKEVTTKKKAKKPLVFTARATRLFLTFWLLIALSFYFLKKNFQAPEVFSAEVLILTAL
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LPVYMALSLVITFPLEKFIQFLYYKSAQRKIRNRKDLITIGITGSYGKTSTKHFITAVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
EKFSVLMTPESYNTPMGVTKVIRNELKDTHQIFVCEMGARQRGDIKVLVKLANPKIGVIT
CCCEEEECCCCCCCCHHHHHHHHHHHCHHHEEEEEECCCCCCCCEEEEEEECCCCEEEEE
SIGPQHLETFGTIENVAKTKFELVEGLPEDGIAVLNGDNYYCRTFGEKFAVRKLFYGTES
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHCCCCC
PIERKYFLWAENIINTRQGLKFDAVTEDGMRIKCEVPLLGRHNVSNLLAAISVALALGLN
CCCHHHHEEHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCC
EEEIKRGLSRIEPVPHRLQLISTPHGVTVIDDAFNSNPEGAKEALACLKEMDAKRRIIVT
HHHHHHHHHHCCCCCCHHEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEC
PGMVELGEKEYEENKNLGKNIAEVCDYAILVGKKRSRPIFEGLKEASFPEEKVFVVANLD
CCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCH
EATKVLQTLLQPGDVVLFENDLPDNYEG
HHHHHHHHHHCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]