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The map label for this gene is DdlA2

Identifier: 20808895

GI number: 20808895

Start: 2434757

End: 2435926

Strand: Reverse

Name: DdlA2

Synonym: TTE2545

Alternate gene names: 20808895

Gene position: 2435926-2434757 (Counterclockwise)

Preceding gene: 20808896

Following gene: 20808894

Centisome position: 90.57

GC content: 39.4

Gene sequence:

>1170_bases
ATGTTGAAAGTGGCTGTGATGTTTGGAGGAAAATCAGTGGAGCACGAGGTTTCTATAATTACAGGGCTCCAGGTCATTGA
GAATATGAACAAGGAAAAGTATGAGCCAATACCGGTATACGTGGGAAGAGACGGGAGATGGTATACTGGCAAAGGCCTTT
TGGACATTTCTAATTACAAAGACATACCTTCCCTTTTAAAGAAATGCGAACAGGTTGTGCTGCCTCCTGTGCCAGGGCTT
AACAAATTGTATTACTACCCTTTCAGGAAGAAATTCATGTCAAAAGAGTATGATTACATAGACGTAGATGTCATTTTTCC
TGCTTTTCACGGAACTCACGGCGAAGATGGCACTATGCAAGGATTATTTCAACTAGCAAATATACCCTATGTGGGGAGCG
GAGTAGTAGGCTCAGCGACAGGAATGGATAAGGTGATAATGAAGGATATTTTCAAAGCGAATAATCTTCCCATAGTTAAT
TACCTGTGGTTTTTGAGAAAAGAATACGAAAAGAACAAAGAAGCTATGCTAGATTTGATTGAGAGCAAGCTAAAATATCC
GATGTTTGTCAAGCCGGCTAATTTGGGTTCCAGTATAGGCATAAGCAAGGCCAAAAATAGGGATGAGCTTTTTGACGCTA
TAGAAATTGCCATTTATTACGACAGAAAGATAATAGTGGAGGAAGCTGTTAAAGATCCCATGGAAGTGAACTGCTCTGTT
ATGGGCATTGACAGGGATTTGGAGATATCTTTATGCGAAATGCCTGTACCCTGGGAAGAGTTTTTGAGTTACCAGGACAA
ATACATGAGAAAAGAAAAAGGCGGCAAAGGAAGTACTACGAGGCAGATTCCTGCCCCCATTCCTGAAGAAAAGACTAAAG
AAATACAGGAACTGGCTAAAAAAGTGTTTCAAATTTTAGATTGCAGTGGATTAGCTAGAATAGACTTTTTGCTAGAAAAA
GAGACCATGAAGGTGTATGTAAATGAAATAAATACAATTCCTGGTTCATTTGCCTTCTACTTGTGGAAACCTTTGGGGAT
TTCTTTTGAGAAATTGATTGATAGGCTTATTGAGTATGCCGTTGAAAGGCATAAAGAGTATGCCAGAAATATGTATACTT
TTGAGACTACTTTGTTGCTTAAGGCGGCCGACAGAGGAACAAAAATGTAA

Upstream 100 bases:

>100_bases
ATGAGGCTACAAAAGTTTTACAGACGCTACTTCAACCGGGAGATGTGGTGCTTTTTGAAAATGACTTGCCTGATAATTAT
GAAGGATGAGGAGGATTTTT

Downstream 100 bases:

>100_bases
AAATACCCCCTTTTTACTGGGGGTAGATAGTGTAAAATTTCAGTAGTAACGAAGCAGGAAAATATTGTATGATGGCATTG
ATTACGTACCAACCCATCCA

Product: D-alanine-D-alanine ligase and related ATP-grasp enzyme

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLKVAVMFGGKSVEHEVSIITGLQVIENMNKEKYEPIPVYVGRDGRWYTGKGLLDISNYKDIPSLLKKCEQVVLPPVPGL
NKLYYYPFRKKFMSKEYDYIDVDVIFPAFHGTHGEDGTMQGLFQLANIPYVGSGVVGSATGMDKVIMKDIFKANNLPIVN
YLWFLRKEYEKNKEAMLDLIESKLKYPMFVKPANLGSSIGISKAKNRDELFDAIEIAIYYDRKIIVEEAVKDPMEVNCSV
MGIDRDLEISLCEMPVPWEEFLSYQDKYMRKEKGGKGSTTRQIPAPIPEEKTKEIQELAKKVFQILDCSGLARIDFLLEK
ETMKVYVNEINTIPGSFAFYLWKPLGISFEKLIDRLIEYAVERHKEYARNMYTFETTLLLKAADRGTKM

Sequences:

>Translated_389_residues
MLKVAVMFGGKSVEHEVSIITGLQVIENMNKEKYEPIPVYVGRDGRWYTGKGLLDISNYKDIPSLLKKCEQVVLPPVPGL
NKLYYYPFRKKFMSKEYDYIDVDVIFPAFHGTHGEDGTMQGLFQLANIPYVGSGVVGSATGMDKVIMKDIFKANNLPIVN
YLWFLRKEYEKNKEAMLDLIESKLKYPMFVKPANLGSSIGISKAKNRDELFDAIEIAIYYDRKIIVEEAVKDPMEVNCSV
MGIDRDLEISLCEMPVPWEEFLSYQDKYMRKEKGGKGSTTRQIPAPIPEEKTKEIQELAKKVFQILDCSGLARIDFLLEK
ETMKVYVNEINTIPGSFAFYLWKPLGISFEKLIDRLIEYAVERHKEYARNMYTFETTLLLKAADRGTKM
>Mature_389_residues
MLKVAVMFGGKSVEHEVSIITGLQVIENMNKEKYEPIPVYVGRDGRWYTGKGLLDISNYKDIPSLLKKCEQVVLPPVPGL
NKLYYYPFRKKFMSKEYDYIDVDVIFPAFHGTHGEDGTMQGLFQLANIPYVGSGVVGSATGMDKVIMKDIFKANNLPIVN
YLWFLRKEYEKNKEAMLDLIESKLKYPMFVKPANLGSSIGISKAKNRDELFDAIEIAIYYDRKIIVEEAVKDPMEVNCSV
MGIDRDLEISLCEMPVPWEEFLSYQDKYMRKEKGGKGSTTRQIPAPIPEEKTKEIQELAKKVFQILDCSGLARIDFLLEK
ETMKVYVNEINTIPGSFAFYLWKPLGISFEKLIDRLIEYAVERHKEYARNMYTFETTLLLKAADRGTKM

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=376, Percent_Identity=36.1702127659575, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1786280, Length=363, Percent_Identity=28.9256198347107, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_THETN (Q8R778)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_624066.1
- ProteinModelPortal:   Q8R778
- SMR:   Q8R778
- GeneID:   997660
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2545
- NMPDR:   fig|273068.3.peg.2019
- HOGENOM:   HBG427092
- OMA:   CKAINAY
- ProtClustDB:   CLSK2754819
- BioCyc:   TTEN273068:TTE2545-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 44677; Mature: 44677

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVAVMFGGKSVEHEVSIITGLQVIENMNKEKYEPIPVYVGRDGRWYTGKGLLDISNYK
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCEEECCCCC
DIPSLLKKCEQVVLPPVPGLNKLYYYPFRKKFMSKEYDYIDVDVIFPAFHGTHGEDGTMQ
CHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHH
GLFQLANIPYVGSGVVGSATGMDKVIMKDIFKANNLPIVNYLWFLRKEYEKNKEAMLDLI
HHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
ESKLKYPMFVKPANLGSSIGISKAKNRDELFDAIEIAIYYDRKIIVEEAVKDPMEVNCSV
HHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCHHHHHHHCCCHHCCEEE
MGIDRDLEISLCEMPVPWEEFLSYQDKYMRKEKGGKGSTTRQIPAPIPEEKTKEIQELAK
EECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KVFQILDCSGLARIDFLLEKETMKVYVNEINTIPGSFAFYLWKPLGISFEKLIDRLIEYA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEHHCCCCCHHHHHHHHHHHH
VERHKEYARNMYTFETTLLLKAADRGTKM
HHHHHHHHHHHHEEEHEEEEEECCCCCCC
>Mature Secondary Structure
MLKVAVMFGGKSVEHEVSIITGLQVIENMNKEKYEPIPVYVGRDGRWYTGKGLLDISNYK
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCEEECCCCC
DIPSLLKKCEQVVLPPVPGLNKLYYYPFRKKFMSKEYDYIDVDVIFPAFHGTHGEDGTMQ
CHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHH
GLFQLANIPYVGSGVVGSATGMDKVIMKDIFKANNLPIVNYLWFLRKEYEKNKEAMLDLI
HHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
ESKLKYPMFVKPANLGSSIGISKAKNRDELFDAIEIAIYYDRKIIVEEAVKDPMEVNCSV
HHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHEEEEEECCCHHHHHHHCCCHHCCEEE
MGIDRDLEISLCEMPVPWEEFLSYQDKYMRKEKGGKGSTTRQIPAPIPEEKTKEIQELAK
EECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KVFQILDCSGLARIDFLLEKETMKVYVNEINTIPGSFAFYLWKPLGISFEKLIDRLIEYA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEHHCCCCCHHHHHHHHHHHH
VERHKEYARNMYTFETTLLLKAADRGTKM
HHHHHHHHHHHHEEEHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336