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The map label for this gene is HyfC [H]

Identifier: 20808130

GI number: 20808130

Start: 1653039

End: 1653980

Strand: Reverse

Name: HyfC [H]

Synonym: TTE1705

Alternate gene names: 20808130

Gene position: 1653980-1653039 (Counterclockwise)

Preceding gene: 20808131

Following gene: 161760531

Centisome position: 61.5

GC content: 43.21

Gene sequence:

>942_bases
ATGTTGGATGGCAAATTGTTTTTTGTGGGCTTGTTGCAGGCATTATTGCTAATGGCAGTAGCGCCATTTTTTTCGGGCTT
TGCCCGTGTTTTAAGGGCGAAAATTCATTCGAGGAAAGGTCCCAGTATATTTCAGGATTACCGCGATATTTTCAAATTAA
TAAAAAGACAGCGAGTAGTGCCGGCACAGAGTAGCTGGATTTTTTGCTTGACTCCTTACGTAATGATGGCGACAATTTTG
CTCATCGCAATGATGACCCCAATTTTAACTTTACAATCGCCATTAAGGATGGTGGGGGATTTAATTGCAATTGTTTATTT
GTTTGCTTTGGGGCGGTTTTTTTTCGCTGCTGCAGGTCTAGATTCTGGTAGTGGATTTGCGGGGATAGGTGCCAGCCGTG
AAATGACACTGGCAGCGCTGGTGGAACCAACTATTATACTGGTTTTGTTTGTGATGGGGCTCCTTGCCGGTTCGACTGAT
ATAGGTGTAATAAGTCAAAAGGTGGCAACGGGTGAAATATCATATTATAGTCCCACAGTTTGGCTTGCGATGGCAGCTTT
TTCTATTGCCGCTTTTATTGAAACTGGTAAATTGCCTTTTGATTTGGCAGAAGCAGAACAGGAAATCCAGGAAGGACCGC
TTACGGAATATTCGGGAGGCTCCCTCGCTCTTCTCAAATGGAGTTTGTCTATGAAGCAGGTTGTGGTGATTGCTTTGTTT
ATGGCCATCTTTTTCCCATTTGGAAATGCGGCAGTTGTAAATGGTTTTTCTATTCTGATGTCTACGGCAGTATTTTTGCT
GAAAGTTGCTATCTTTTACGTGATTGCAGCTTTGCTGGAAAACAGTATGGCCAGACTACTGCTTTTCAGGGCCCATGAAG
TAACCTGGGCTGCCTTTGGGATCGCTTTGCTGTCTTTTGTTTTCTTCCTTGTTAATGTCTAA

Upstream 100 bases:

>100_bases
CACAAACTTTCCAGACAGGTAATGTCCGGATGTACTGCCTGTACATAATCCTGACTCTTGGGGTTCTTTTGATAGCGACG
GTGAGATAGGAGGTAAAAAG

Downstream 100 bases:

>100_bases
AAGATTCGGGAGGAGATACTTAATGTCTGGTACTAATATAGTTAATGTTTTATCTGTGCTGCTCATCCTGACCTCTATGT
TAGTAGTGGAAACAAGGAAG

Product: formate hydrogenlyase subunit 4

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLDGKLFFVGLLQALLLMAVAPFFSGFARVLRAKIHSRKGPSIFQDYRDIFKLIKRQRVVPAQSSWIFCLTPYVMMATIL
LIAMMTPILTLQSPLRMVGDLIAIVYLFALGRFFFAAAGLDSGSGFAGIGASREMTLAALVEPTIILVLFVMGLLAGSTD
IGVISQKVATGEISYYSPTVWLAMAAFSIAAFIETGKLPFDLAEAEQEIQEGPLTEYSGGSLALLKWSLSMKQVVVIALF
MAIFFPFGNAAVVNGFSILMSTAVFLLKVAIFYVIAALLENSMARLLLFRAHEVTWAAFGIALLSFVFFLVNV

Sequences:

>Translated_313_residues
MLDGKLFFVGLLQALLLMAVAPFFSGFARVLRAKIHSRKGPSIFQDYRDIFKLIKRQRVVPAQSSWIFCLTPYVMMATIL
LIAMMTPILTLQSPLRMVGDLIAIVYLFALGRFFFAAAGLDSGSGFAGIGASREMTLAALVEPTIILVLFVMGLLAGSTD
IGVISQKVATGEISYYSPTVWLAMAAFSIAAFIETGKLPFDLAEAEQEIQEGPLTEYSGGSLALLKWSLSMKQVVVIALF
MAIFFPFGNAAVVNGFSILMSTAVFLLKVAIFYVIAALLENSMARLLLFRAHEVTWAAFGIALLSFVFFLVNV
>Mature_313_residues
MLDGKLFFVGLLQALLLMAVAPFFSGFARVLRAKIHSRKGPSIFQDYRDIFKLIKRQRVVPAQSSWIFCLTPYVMMATIL
LIAMMTPILTLQSPLRMVGDLIAIVYLFALGRFFFAAAGLDSGSGFAGIGASREMTLAALVEPTIILVLFVMGLLAGSTD
IGVISQKVATGEISYYSPTVWLAMAAFSIAAFIETGKLPFDLAEAEQEIQEGPLTEYSGGSLALLKWSLSMKQVVVIALF
MAIFFPFGNAAVVNGFSILMSTAVFLLKVAIFYVIAALLENSMARLLLFRAHEVTWAAFGIALLSFVFFLVNV

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=300, Percent_Identity=50.6666666666667, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI87082115, Length=314, Percent_Identity=51.2738853503185, Blast_Score=280, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 34118; Mature: 34118

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDGKLFFVGLLQALLLMAVAPFFSGFARVLRAKIHSRKGPSIFQDYRDIFKLIKRQRVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
PAQSSWIFCLTPYVMMATILLIAMMTPILTLQSPLRMVGDLIAIVYLFALGRFFFAAAGL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSGSGFAGIGASREMTLAALVEPTIILVLFVMGLLAGSTDIGVISQKVATGEISYYSPTV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHH
WLAMAAFSIAAFIETGKLPFDLAEAEQEIQEGPLTEYSGGSLALLKWSLSMKQVVVIALF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHH
MAIFFPFGNAAVVNGFSILMSTAVFLLKVAIFYVIAALLENSMARLLLFRAHEVTWAAFG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALLSFVFFLVNV
HHHHHHHHHHHCC
>Mature Secondary Structure
MLDGKLFFVGLLQALLLMAVAPFFSGFARVLRAKIHSRKGPSIFQDYRDIFKLIKRQRVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
PAQSSWIFCLTPYVMMATILLIAMMTPILTLQSPLRMVGDLIAIVYLFALGRFFFAAAGL
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSGSGFAGIGASREMTLAALVEPTIILVLFVMGLLAGSTDIGVISQKVATGEISYYSPTV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHH
WLAMAAFSIAAFIETGKLPFDLAEAEQEIQEGPLTEYSGGSLALLKWSLSMKQVVVIALF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHH
MAIFFPFGNAAVVNGFSILMSTAVFLLKVAIFYVIAALLENSMARLLLFRAHEVTWAAFG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALLSFVFFLVNV
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]