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The map label for this gene is hyfE [H]

Identifier: 161760531

GI number: 161760531

Start: 1652369

End: 1653016

Strand: Reverse

Name: hyfE [H]

Synonym: TTE1704

Alternate gene names: 161760531

Gene position: 1653016-1652369 (Counterclockwise)

Preceding gene: 20808130

Following gene: 20808128

Centisome position: 61.46

GC content: 40.43

Gene sequence:

>648_bases
ATGTCTGGTACTAATATAGTTAATGTTTTATCTGTGCTGCTCATCCTGACCTCTATGTTAGTAGTGGAAACAAGGAAGTT
ACGGATGGCGGCATATATGTACAGATTCCAATCTTTAGTTTTGGTTACTATTTTTCTTTCTATTGCTGTGTTTATGGAGG
CAAAACCTCTGTACATTTGGTCAATCACTGCCCTTATTACAAAAACTTTTTTAGTGCCATATCTCATCCTCAAGGTTATC
GAAAGGGTAAAAGAACAGGAAGAGGGAGTGGCTATTGACAGGAAATTAACTATTATTCTGGCTGTGGGCATTTTAGCTTT
ATCCTTCGCAGTGGTGGAACCTTTTCACATGCAGGCAGTACTAAAATTGAGAGTGGCCCTGGCAGTTTCCATAGCTCATT
TTATGCTGGGCCTGCTCTGCATACTCGCCAGAAAAAATGCCCTCAAACAGATATTGGGTTACTGTCTTATGGAGAATGGG
TCCCATTTGACTCTGGCTATTATGGCTTACAATGCCCCTGAAACGGTGGAAATTGGCATTCTGACAGACGCTGTTTTTGC
TGTATTGATTATGACAATTATTGCCAGACGCCTTTTTAAAGATTTTGGTACTCTGGACACTGATAAGCTCACACTACTAA
AAGGTTAG

Upstream 100 bases:

>100_bases
TTCAGGGCCCATGAAGTAACCTGGGCTGCCTTTGGGATCGCTTTGCTGTCTTTTGTTTTCTTCCTTGTTAATGTCTAAAA
GATTCGGGAGGAGATACTTA

Downstream 100 bases:

>100_bases
GTGATTGAGGTGGAAAAAAGTATTTTGTTTTACTTGTTGCTTGCGGTACCGCTGGCTACCTCCTTTCTGGCTTTTGGCAT
AAAGTCCCGACGGGTGGTAG

Product: hydrogenase 4 membrane subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSGTNIVNVLSVLLILTSMLVVETRKLRMAAYMYRFQSLVLVTIFLSIAVFMEAKPLYIWSITALITKTFLVPYLILKVI
ERVKEQEEGVAIDRKLTIILAVGILALSFAVVEPFHMQAVLKLRVALAVSIAHFMLGLLCILARKNALKQILGYCLMENG
SHLTLAIMAYNAPETVEIGILTDAVFAVLIMTIIARRLFKDFGTLDTDKLTLLKG

Sequences:

>Translated_215_residues
MSGTNIVNVLSVLLILTSMLVVETRKLRMAAYMYRFQSLVLVTIFLSIAVFMEAKPLYIWSITALITKTFLVPYLILKVI
ERVKEQEEGVAIDRKLTIILAVGILALSFAVVEPFHMQAVLKLRVALAVSIAHFMLGLLCILARKNALKQILGYCLMENG
SHLTLAIMAYNAPETVEIGILTDAVFAVLIMTIIARRLFKDFGTLDTDKLTLLKG
>Mature_214_residues
SGTNIVNVLSVLLILTSMLVVETRKLRMAAYMYRFQSLVLVTIFLSIAVFMEAKPLYIWSITALITKTFLVPYLILKVIE
RVKEQEEGVAIDRKLTIILAVGILALSFAVVEPFHMQAVLKLRVALAVSIAHFMLGLLCILARKNALKQILGYCLMENGS
HLTLAIMAYNAPETVEIGILTDAVFAVLIMTIIARRLFKDFGTLDTDKLTLLKG

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788830, Length=216, Percent_Identity=45.3703703703704, Blast_Score=187, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001133 [H]

Pfam domain/function: PF00420 Oxidored_q2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23932; Mature: 23800

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTNIVNVLSVLLILTSMLVVETRKLRMAAYMYRFQSLVLVTIFLSIAVFMEAKPLYIW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SITALITKTFLVPYLILKVIERVKEQEEGVAIDRKLTIILAVGILALSFAVVEPFHMQAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LKLRVALAVSIAHFMLGLLCILARKNALKQILGYCLMENGSHLTLAIMAYNAPETVEIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEHH
LTDAVFAVLIMTIIARRLFKDFGTLDTDKLTLLKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
SGTNIVNVLSVLLILTSMLVVETRKLRMAAYMYRFQSLVLVTIFLSIAVFMEAKPLYIW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SITALITKTFLVPYLILKVIERVKEQEEGVAIDRKLTIILAVGILALSFAVVEPFHMQAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LKLRVALAVSIAHFMLGLLCILARKNALKQILGYCLMENGSHLTLAIMAYNAPETVEIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEHH
LTDAVFAVLIMTIIARRLFKDFGTLDTDKLTLLKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]