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The map label for this gene is HyfB2 [H]

Identifier: 20808131

GI number: 20808131

Start: 1653992

End: 1656013

Strand: Reverse

Name: HyfB2 [H]

Synonym: TTE1706

Alternate gene names: 20808131

Gene position: 1656013-1653992 (Counterclockwise)

Preceding gene: 20808132

Following gene: 20808130

Centisome position: 61.57

GC content: 42.68

Gene sequence:

>2022_bases
TTGATGACTGTTCAACAGTTATTTTTGTTATCTATTTTCTTATATGTTGTAGGAGCACTTATCTCTTTGGGATTAAATAG
GGACGGCAAAATTGCAAATTATGTTTCTGGAACAACTGCTTTCTTGGCAGGATGCACTGGAGTCGCTTCAGCTGTTCCGG
TCCTTATTGAAGGGAAAGGTTTTATATTGGAAGGCATGGAGTTTATCCCTTTTGGGCGATTTACAATTAATGTTGATCCT
CTTTCAGCTTTTATGACATTGGTTATTTCTCTACTTAGTATTGCCACAGGTGTTTATTCTATTTCTTATCAGGAAGAGTA
TATTGGGAAAGGCACCGGCGTAATGGGTTTTTTAAATAATATCTTTATTGTTTCAATGATTTTGGTAGTTACTAGTGACA
ACGCTTTCTATTTTTTAGTTTTTTGGGAGCTGATGACGCTGGTTTCTTATTTCCTGGTTTGTTTTGAACAGGAAAACAGA
GAGGCAATAAAAGCTGGATTTGTGTATTTTGTAATGGCCCATGTTGGCACAGTTTTGATAATGATTTCGTTTATATTATT
TTATGTTTATACCGGTACTTTTGATTTTGCCGCTTTTAGGAATGCAAATCTTTCGCCTGTTATCAAGAATTTAGCTTTTC
TTCTGGCTTTTTTGGGATTTGGGGTTAAGGCAGGCATCGTTCCCTTCCATTTCTGGCTGCCTCAGGCTCATCCTGCCGCT
CCTTCTAATGTTTCTGCTCTTATGTCTGGTGTCATGATTAAGACAGCTATTTACGGCATTATAAGAGTTGGGGTGGACTT
TTTGGGGGCTGCTACATGGTGGTGGGGCCTTACGGTCCTTGCCTTTGGGGCTATTTCAGCAGTTTTTGGAGTGCTTTATG
CTGTTAGTGAAAATGACCTTAAACGCTTGTTGGCCTATTCCAGCATAGAGAATGTGGGAATTATCCTGATGGGAGTCGGT
GCGGGAATGATGGGAATAGCCACAGGATACGTTAGTGTAGGCATATTAGGTATTCTGGCGGGGCTTTATCATTTGATAAA
TCACGCTGTCTTCAAAGGGTTGCTTTTTCTTGGGGCGGGCTCGGTAGTTTACCGCCTCCACACAAGGAATATGGAGGAGA
TGGGAGGATTGGCCAGATATATGCCATGGACTGGATTGACCTTCTTTATAGGCGCTTTGTCGATTTCCGCCCTTCCTCCG
CTAAATGGCTTTGTAAGCAAATGGTTTACATACCATACTCTGTTTGCTGTCAGCACAAAAGGGAGTGTGCTGATTAGAAT
GGCATTTCCTCTCGCCGCAGTCGCTTTAGCCTTAACAGGAGCTTTGACGTCTCTGTGTTTTGTAAAAGCATATGGAGTGA
CTTTTACTGGACCTTTTCGAGGTGGCAATGAATTAAAGATTAAAGAGGTTCCTGTGACCATGCTTATAGGGATGGGGCTT
TTAGCAATAGGCTGTTTTGTTTTGGGGCTTGGCGCGCCGGTTGGGGCTGGATATATTGCGAATGTGGCTTCAGCGTTGCT
ACATGTATCTTCACCCAAGGTAAGTGACGGTCTGTCGATATTTCCAGCAAGCAGTGAGCAGGCCATGCTTTCTACGCCAC
TTGTGCTTGTTTTTGTCGTAGGACTTGCGATATTGCCCCTATTAATTATTGGAACAGGTGGAAAGCGAGCAGGAAGGCGA
GTGGACTTTGAACCCTGGGCATGTGGCTATAAATATTCATCTCGCATGGCGTGGACATCCACAGCCTTTGCTCACTCACT
GTACCCGCTTTTCAGTCCAGTATATTTGCTTAGGACTGCATTTACAGAAGCTGGTCGTTTCATTATTTTGCTTTTCAAAA
AGGCGATGGCGTATATATCACAGATAGAGCTGATGTGGGAACGTTATATATACTATCCTTTGCTTCATTTCATGGTCTAT
ATAGGTAAACGTGCACAAACTTTCCAGACAGGTAATGTCCGGATGTACTGCCTGTACATAATCCTGACTCTTGGGGTTCT
TTTGATAGCGACGGTGAGATAG

Upstream 100 bases:

>100_bases
AAATCGTCTTCCAACTAATAGAAAGTTAGAAAATAAAAAATTAAACAAGTTTAAAGAACTACTGTTATTATGTGGATATT
TTGAAGGCAGGAGGTAAAAC

Downstream 100 bases:

>100_bases
GAGGTAAAAAGATGTTGGATGGCAAATTGTTTTTTGTGGGCTTGTTGCAGGCATTATTGCTAATGGCAGTAGCGCCATTT
TTTTCGGGCTTTGCCCGTGT

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MMTVQQLFLLSIFLYVVGALISLGLNRDGKIANYVSGTTAFLAGCTGVASAVPVLIEGKGFILEGMEFIPFGRFTINVDP
LSAFMTLVISLLSIATGVYSISYQEEYIGKGTGVMGFLNNIFIVSMILVVTSDNAFYFLVFWELMTLVSYFLVCFEQENR
EAIKAGFVYFVMAHVGTVLIMISFILFYVYTGTFDFAAFRNANLSPVIKNLAFLLAFLGFGVKAGIVPFHFWLPQAHPAA
PSNVSALMSGVMIKTAIYGIIRVGVDFLGAATWWWGLTVLAFGAISAVFGVLYAVSENDLKRLLAYSSIENVGIILMGVG
AGMMGIATGYVSVGILGILAGLYHLINHAVFKGLLFLGAGSVVYRLHTRNMEEMGGLARYMPWTGLTFFIGALSISALPP
LNGFVSKWFTYHTLFAVSTKGSVLIRMAFPLAAVALALTGALTSLCFVKAYGVTFTGPFRGGNELKIKEVPVTMLIGMGL
LAIGCFVLGLGAPVGAGYIANVASALLHVSSPKVSDGLSIFPASSEQAMLSTPLVLVFVVGLAILPLLIIGTGGKRAGRR
VDFEPWACGYKYSSRMAWTSTAFAHSLYPLFSPVYLLRTAFTEAGRFIILLFKKAMAYISQIELMWERYIYYPLLHFMVY
IGKRAQTFQTGNVRMYCLYIILTLGVLLIATVR

Sequences:

>Translated_673_residues
MMTVQQLFLLSIFLYVVGALISLGLNRDGKIANYVSGTTAFLAGCTGVASAVPVLIEGKGFILEGMEFIPFGRFTINVDP
LSAFMTLVISLLSIATGVYSISYQEEYIGKGTGVMGFLNNIFIVSMILVVTSDNAFYFLVFWELMTLVSYFLVCFEQENR
EAIKAGFVYFVMAHVGTVLIMISFILFYVYTGTFDFAAFRNANLSPVIKNLAFLLAFLGFGVKAGIVPFHFWLPQAHPAA
PSNVSALMSGVMIKTAIYGIIRVGVDFLGAATWWWGLTVLAFGAISAVFGVLYAVSENDLKRLLAYSSIENVGIILMGVG
AGMMGIATGYVSVGILGILAGLYHLINHAVFKGLLFLGAGSVVYRLHTRNMEEMGGLARYMPWTGLTFFIGALSISALPP
LNGFVSKWFTYHTLFAVSTKGSVLIRMAFPLAAVALALTGALTSLCFVKAYGVTFTGPFRGGNELKIKEVPVTMLIGMGL
LAIGCFVLGLGAPVGAGYIANVASALLHVSSPKVSDGLSIFPASSEQAMLSTPLVLVFVVGLAILPLLIIGTGGKRAGRR
VDFEPWACGYKYSSRMAWTSTAFAHSLYPLFSPVYLLRTAFTEAGRFIILLFKKAMAYISQIELMWERYIYYPLLHFMVY
IGKRAQTFQTGNVRMYCLYIILTLGVLLIATVR
>Mature_673_residues
MMTVQQLFLLSIFLYVVGALISLGLNRDGKIANYVSGTTAFLAGCTGVASAVPVLIEGKGFILEGMEFIPFGRFTINVDP
LSAFMTLVISLLSIATGVYSISYQEEYIGKGTGVMGFLNNIFIVSMILVVTSDNAFYFLVFWELMTLVSYFLVCFEQENR
EAIKAGFVYFVMAHVGTVLIMISFILFYVYTGTFDFAAFRNANLSPVIKNLAFLLAFLGFGVKAGIVPFHFWLPQAHPAA
PSNVSALMSGVMIKTAIYGIIRVGVDFLGAATWWWGLTVLAFGAISAVFGVLYAVSENDLKRLLAYSSIENVGIILMGVG
AGMMGIATGYVSVGILGILAGLYHLINHAVFKGLLFLGAGSVVYRLHTRNMEEMGGLARYMPWTGLTFFIGALSISALPP
LNGFVSKWFTYHTLFAVSTKGSVLIRMAFPLAAVALALTGALTSLCFVKAYGVTFTGPFRGGNELKIKEVPVTMLIGMGL
LAIGCFVLGLGAPVGAGYIANVASALLHVSSPKVSDGLSIFPASSEQAMLSTPLVLVFVVGLAILPLLIIGTGGKRAGRR
VDFEPWACGYKYSSRMAWTSTAFAHSLYPLFSPVYLLRTAFTEAGRFIILLFKKAMAYISQIELMWERYIYYPLLHFMVY
IGKRAQTFQTGNVRMYCLYIILTLGVLLIATVR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=371, Percent_Identity=25.8760107816712, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1788827, Length=675, Percent_Identity=42.5185185185185, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI2367154, Length=527, Percent_Identity=33.5863377609108, Blast_Score=282, Evalue=6e-77,
Organism=Escherichia coli, GI1788831, Length=363, Percent_Identity=32.7823691460055, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1788613, Length=357, Percent_Identity=28.0112044817927, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788614, Length=435, Percent_Identity=28.735632183908, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1788829, Length=364, Percent_Identity=27.1978021978022, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI145693160, Length=291, Percent_Identity=28.1786941580756, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 73084; Mature: 73084

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTVQQLFLLSIFLYVVGALISLGLNRDGKIANYVSGTTAFLAGCTGVASAVPVLIEGKG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHCCEEECCCC
FILEGMEFIPFGRFTINVDPLSAFMTLVISLLSIATGVYSISYQEEYIGKGTGVMGFLNN
CEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHEEEEHHHHHCCCCHHHHHHHH
IFIVSMILVVTSDNAFYFLVFWELMTLVSYFLVCFEQENREAIKAGFVYFVMAHVGTVLI
HHHHHHHHHEECCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MISFILFYVYTGTFDFAAFRNANLSPVIKNLAFLLAFLGFGVKAGIVPFHFWLPQAHPAA
HHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCHHCCCCEEEEECCCCCCCC
PSNVSALMSGVMIKTAIYGIIRVGVDFLGAATWWWGLTVLAFGAISAVFGVLYAVSENDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
KRLLAYSSIENVGIILMGVGAGMMGIATGYVSVGILGILAGLYHLINHAVFKGLLFLGAG
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVVYRLHTRNMEEMGGLARYMPWTGLTFFIGALSISALPPLNGFVSKWFTYHTLFAVSTK
HHEEEEECCCHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECC
GSVLIRMAFPLAAVALALTGALTSLCFVKAYGVTFTGPFRGGNELKIKEVPVTMLIGMGL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCHHHHHHHHH
LAIGCFVLGLGAPVGAGYIANVASALLHVSSPKVSDGLSIFPASSEQAMLSTPLVLVFVV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
GLAILPLLIIGTGGKRAGRRVDFEPWACGYKYSSRMAWTSTAFAHSLYPLFSPVYLLRTA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTEAGRFIILLFKKAMAYISQIELMWERYIYYPLLHFMVYIGKRAQTFQTGNVRMYCLYI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHH
ILTLGVLLIATVR
HHHHHHHHHHCCC
>Mature Secondary Structure
MMTVQQLFLLSIFLYVVGALISLGLNRDGKIANYVSGTTAFLAGCTGVASAVPVLIEGKG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHCCEEECCCC
FILEGMEFIPFGRFTINVDPLSAFMTLVISLLSIATGVYSISYQEEYIGKGTGVMGFLNN
CEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHEEEEHHHHHCCCCHHHHHHHH
IFIVSMILVVTSDNAFYFLVFWELMTLVSYFLVCFEQENREAIKAGFVYFVMAHVGTVLI
HHHHHHHHHEECCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MISFILFYVYTGTFDFAAFRNANLSPVIKNLAFLLAFLGFGVKAGIVPFHFWLPQAHPAA
HHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCHHCCCCEEEEECCCCCCCC
PSNVSALMSGVMIKTAIYGIIRVGVDFLGAATWWWGLTVLAFGAISAVFGVLYAVSENDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
KRLLAYSSIENVGIILMGVGAGMMGIATGYVSVGILGILAGLYHLINHAVFKGLLFLGAG
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVVYRLHTRNMEEMGGLARYMPWTGLTFFIGALSISALPPLNGFVSKWFTYHTLFAVSTK
HHEEEEECCCHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECC
GSVLIRMAFPLAAVALALTGALTSLCFVKAYGVTFTGPFRGGNELKIKEVPVTMLIGMGL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCHHHHHHHHH
LAIGCFVLGLGAPVGAGYIANVASALLHVSSPKVSDGLSIFPASSEQAMLSTPLVLVFVV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
GLAILPLLIIGTGGKRAGRRVDFEPWACGYKYSSRMAWTSTAFAHSLYPLFSPVYLLRTA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTEAGRFIILLFKKAMAYISQIELMWERYIYYPLLHFMVYIGKRAQTFQTGNVRMYCLYI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHH
ILTLGVLLIATVR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]