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The map label for this gene is HyfB [H]

Identifier: 20808128

GI number: 20808128

Start: 1650875

End: 1652359

Strand: Reverse

Name: HyfB [H]

Synonym: TTE1702

Alternate gene names: 20808128

Gene position: 1652359-1650875 (Counterclockwise)

Preceding gene: 161760531

Following gene: 20808127

Centisome position: 61.44

GC content: 43.23

Gene sequence:

>1485_bases
GTGGAAAAAAGTATTTTGTTTTACTTGTTGCTTGCGGTACCGCTGGCTACCTCCTTTCTGGCTTTTGGCATAAAGTCCCG
ACGGGTGGTAGAGGCTGTACACTTTGCAGGAATAAGTATAGAGGCTTTCCTCTCTTTACAGATGGTGCAGTATGTAATAA
GGGAAGAGAGGCTGTTTGCTCTTGGTGATATGCTTTACGCTGATAGTCTGGTGGCGATTTTGCTCCTTATTATTGGAGTT
GTGGGGTTTTTAAACGGTTTTTACTCTATAGGACATATGAGATATGACATTAGCCGTGGCGAAATAGATGAGAAGGAAAT
CTCCTCTTATTACGGCTTTTACCATATACTTTTGTTTACGATGGTGCTTTGCGCTTTATCCAACAACATCGCCATTATGT
GGGTAGCGCTGGAAGCAACTACTCTGGGTTCAGCCTTTTTGGTTGGGTTTTATAAACACAAAGCATCGGCGGAAGCTGCC
TGGAAGTACGTTTTGATCTGCAGCGTTGGGTTGGCCTTTGCGCTTTACGGGACTATTCTAACCTACTCTAATGCTTTTGC
CATGGTGCAGGATGCCCATAAAGCTATGTTGTGGACTGAACTTATAAAAATGGCTCCTCGTTTAGACCAGCAAGTGATGA
AACTGGCTTTTGTGTTCATTTTAATCGGGTTTGGTACCAAAGTGGGTTTAGTTCCCATGCATACATGGCTACCTGATACT
TACAGCGAATCTCCAAGCCCTGTTAGTGCACTGTTATCTGGTGTACTCATGAAATGTGCGCTGTTTGCGATAATAAAGTA
TTACACAATTGCTCTGCAGGCTATTGGTAAAGAGTTTTTAGAGACACTGCTTCTAATTTTTGGACTTGTGTCTGTAGGTG
TTTCGGCTTTTTTTATCCTGGTGCAGAAGGACATCAAAAGGAAATTGGCTTACAGTAGTGTAGAGCATGTAGGCATTATC
GCTACTGGCCTGGGTGTTGGGGGAACCTTTGGAGTACTGGGTGCTCTTCTTCACGCCATAAACCACAGTGTCACTAAATC
CCTTTTATTCTGTACTGCTGGAAATGTGATTGTAAAGTACGGCACAAGAGATTCACAAAGAATTAGAGGAATATTCAAGG
TAGCTCCTGTTACTGCCTTCCTGTGGACAGCGGGGCTTCTTGCGTTAGTGGGCTCACCTCCTTTTAACATTTTCATAAGT
GAGGTCGTAACTGTGATAGCTGGCATAGAAGCTGGCCATTTTTGGGCGATGATATTATTCCTTGTTTTACTTGTGGTTGT
CTTCGTGGCGTTTTTTGTGTTGATAAGCGAGACGGTTATTGGCTCTCCTCCAGAAGATTTACCTAAGGGCGATGTCAATT
GGTTGACATTACTACCAATCGGTATCTTGTTTGTGTTTATGGCAGCGCTGGGGTTACATTTGCCTTCTACTTTAAGTCAA
CTATTGCAAAGGGCAGTGACCATTGTTTTAGGAGGGACGATATAA

Upstream 100 bases:

>100_bases
TGCTGTATTGATTATGACAATTATTGCCAGACGCCTTTTTAAAGATTTTGGTACTCTGGACACTGATAAGCTCACACTAC
TAAAAGGTTAGGTGATTGAG

Downstream 100 bases:

>100_bases
ATGGTTTGGTATGCACTGGGCAGCATCCTTCTACCATTGGTAGGAAGTTTAGTTGTGCTGTTTTTGTCAGAACAACAGGT
AAAAAAGGCAGGTCAGTTGT

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MEKSILFYLLLAVPLATSFLAFGIKSRRVVEAVHFAGISIEAFLSLQMVQYVIREERLFALGDMLYADSLVAILLLIIGV
VGFLNGFYSIGHMRYDISRGEIDEKEISSYYGFYHILLFTMVLCALSNNIAIMWVALEATTLGSAFLVGFYKHKASAEAA
WKYVLICSVGLAFALYGTILTYSNAFAMVQDAHKAMLWTELIKMAPRLDQQVMKLAFVFILIGFGTKVGLVPMHTWLPDT
YSESPSPVSALLSGVLMKCALFAIIKYYTIALQAIGKEFLETLLLIFGLVSVGVSAFFILVQKDIKRKLAYSSVEHVGII
ATGLGVGGTFGVLGALLHAINHSVTKSLLFCTAGNVIVKYGTRDSQRIRGIFKVAPVTAFLWTAGLLALVGSPPFNIFIS
EVVTVIAGIEAGHFWAMILFLVLLVVVFVAFFVLISETVIGSPPEDLPKGDVNWLTLLPIGILFVFMAALGLHLPSTLSQ
LLQRAVTIVLGGTI

Sequences:

>Translated_494_residues
MEKSILFYLLLAVPLATSFLAFGIKSRRVVEAVHFAGISIEAFLSLQMVQYVIREERLFALGDMLYADSLVAILLLIIGV
VGFLNGFYSIGHMRYDISRGEIDEKEISSYYGFYHILLFTMVLCALSNNIAIMWVALEATTLGSAFLVGFYKHKASAEAA
WKYVLICSVGLAFALYGTILTYSNAFAMVQDAHKAMLWTELIKMAPRLDQQVMKLAFVFILIGFGTKVGLVPMHTWLPDT
YSESPSPVSALLSGVLMKCALFAIIKYYTIALQAIGKEFLETLLLIFGLVSVGVSAFFILVQKDIKRKLAYSSVEHVGII
ATGLGVGGTFGVLGALLHAINHSVTKSLLFCTAGNVIVKYGTRDSQRIRGIFKVAPVTAFLWTAGLLALVGSPPFNIFIS
EVVTVIAGIEAGHFWAMILFLVLLVVVFVAFFVLISETVIGSPPEDLPKGDVNWLTLLPIGILFVFMAALGLHLPSTLSQ
LLQRAVTIVLGGTI
>Mature_494_residues
MEKSILFYLLLAVPLATSFLAFGIKSRRVVEAVHFAGISIEAFLSLQMVQYVIREERLFALGDMLYADSLVAILLLIIGV
VGFLNGFYSIGHMRYDISRGEIDEKEISSYYGFYHILLFTMVLCALSNNIAIMWVALEATTLGSAFLVGFYKHKASAEAA
WKYVLICSVGLAFALYGTILTYSNAFAMVQDAHKAMLWTELIKMAPRLDQQVMKLAFVFILIGFGTKVGLVPMHTWLPDT
YSESPSPVSALLSGVLMKCALFAIIKYYTIALQAIGKEFLETLLLIFGLVSVGVSAFFILVQKDIKRKLAYSSVEHVGII
ATGLGVGGTFGVLGALLHAINHSVTKSLLFCTAGNVIVKYGTRDSQRIRGIFKVAPVTAFLWTAGLLALVGSPPFNIFIS
EVVTVIAGIEAGHFWAMILFLVLLVVVFVAFFVLISETVIGSPPEDLPKGDVNWLTLLPIGILFVFMAALGLHLPSTLSQ
LLQRAVTIVLGGTI

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=348, Percent_Identity=25.8620689655172, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI251831116, Length=185, Percent_Identity=28.6486486486486, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788831, Length=498, Percent_Identity=50.6024096385542, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1788827, Length=374, Percent_Identity=33.1550802139037, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788829, Length=446, Percent_Identity=28.2511210762332, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI2367154, Length=354, Percent_Identity=28.2485875706215, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788614, Length=343, Percent_Identity=29.1545189504373, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788613, Length=306, Percent_Identity=24.8366013071895, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI145693160, Length=377, Percent_Identity=25.4641909814324, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53908; Mature: 53908

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSILFYLLLAVPLATSFLAFGIKSRRVVEAVHFAGISIEAFLSLQMVQYVIREERLFA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LGDMLYADSLVAILLLIIGVVGFLNGFYSIGHMRYDISRGEIDEKEISSYYGFYHILLFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHH
MVLCALSNNIAIMWVALEATTLGSAFLVGFYKHKASAEAAWKYVLICSVGLAFALYGTIL
HHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TYSNAFAMVQDAHKAMLWTELIKMAPRLDQQVMKLAFVFILIGFGTKVGLVPMHTWLPDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHCCCCC
YSESPSPVSALLSGVLMKCALFAIIKYYTIALQAIGKEFLETLLLIFGLVSVGVSAFFIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQKDIKRKLAYSSVEHVGIIATGLGVGGTFGVLGALLHAINHSVTKSLLFCTAGNVIVKY
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GTRDSQRIRGIFKVAPVTAFLWTAGLLALVGSPPFNIFISEVVTVIAGIEAGHFWAMILF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
LVLLVVVFVAFFVLISETVIGSPPEDLPKGDVNWLTLLPIGILFVFMAALGLHLPSTLSQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
LLQRAVTIVLGGTI
HHHHHHHHHHCCCC
>Mature Secondary Structure
MEKSILFYLLLAVPLATSFLAFGIKSRRVVEAVHFAGISIEAFLSLQMVQYVIREERLFA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LGDMLYADSLVAILLLIIGVVGFLNGFYSIGHMRYDISRGEIDEKEISSYYGFYHILLFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHH
MVLCALSNNIAIMWVALEATTLGSAFLVGFYKHKASAEAAWKYVLICSVGLAFALYGTIL
HHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
TYSNAFAMVQDAHKAMLWTELIKMAPRLDQQVMKLAFVFILIGFGTKVGLVPMHTWLPDT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHCCCCC
YSESPSPVSALLSGVLMKCALFAIIKYYTIALQAIGKEFLETLLLIFGLVSVGVSAFFIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQKDIKRKLAYSSVEHVGIIATGLGVGGTFGVLGALLHAINHSVTKSLLFCTAGNVIVKY
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GTRDSQRIRGIFKVAPVTAFLWTAGLLALVGSPPFNIFISEVVTVIAGIEAGHFWAMILF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
LVLLVVVFVAFFVLISETVIGSPPEDLPKGDVNWLTLLPIGILFVFMAALGLHLPSTLSQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
LLQRAVTIVLGGTI
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]