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The map label for this gene is LipB

Identifier: 20808099

GI number: 20808099

Start: 1620460

End: 1621146

Strand: Reverse

Name: LipB

Synonym: TTE1673

Alternate gene names: 20808099

Gene position: 1621146-1620460 (Counterclockwise)

Preceding gene: 20808100

Following gene: 20808098

Centisome position: 60.28

GC content: 40.03

Gene sequence:

>687_bases
TTGAGGAGAGGAGAAGTATTAAAATTAGGTGTGGTTCCTTATCTTGAAGGGAAAGAGATACAGCTTAAGGCTTTTGAAAA
AGTTAAAAAGGAAGAAACAGACGGTATTTTGATATTACTTCAGCATAAGCCAGTATACACCATAGGTGTTTCAGGTGGAT
ATGATGAAGACATACTGGTCCCCATTGATTACATAAAAGAAAAGGCAGAGCTTTACAAAGTAGAAAGGGGAGGAAAAATA
ACTTTTCACGGGCCTGGACAGGTGGTAGCCTATCCCATTTTTAACCTGGCAAAATGGCAGAAAGATGTTCACCTTTTTGT
TCATAATTTAGAAGAAGCTGTAATAAGGCTTCTGAGGGAATATGGGATTATCGCCGGACGCAAGGAAAAGTACACGGGTG
TGTGGGTTGGAGATGAAAAGATATGCGCAATAGGCATTGCGGTTAAAAGGTGGATAACTTGGCACGGTATAGCGCTCAAT
GTTAACACTGACCTGTCTTACTTTGGCCTTATTAACGCTTGTGGAATTACAGAGTTTGGTGTCACTTCCATGAAAAAGCT
TGGAATAGAAGTTGAAATAGAAGAAGTGATGGACAGACTTGTGGACAAGTTTGAAGAGGTGTTCGAAATAAAATTTGAAG
AGATTGATTTAACTAGATTGGCGGTGGTTGACAGTGCGAAAGCCTGA

Upstream 100 bases:

>100_bases
AGCATACATGCTCATCCTACACTTTCTGAGAGTATAAAAGAGGCTGCAGAGGATGCTTTAGGGATGCCAATAAACAAAGG
TTAAAATAGGGGGAATTGTT

Downstream 100 bases:

>100_bases
ATGGCTTAAAGTGAGAATTTTAAGTGAGGATTTAAACAGGATGGAAGCTTTTTTGAAAAATATGGCCTTAAATACCGTAT
GTCAAAGTGCAAACTGCCCA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MRRGEVLKLGVVPYLEGKEIQLKAFEKVKKEETDGILILLQHKPVYTIGVSGGYDEDILVPIDYIKEKAELYKVERGGKI
TFHGPGQVVAYPIFNLAKWQKDVHLFVHNLEEAVIRLLREYGIIAGRKEKYTGVWVGDEKICAIGIAVKRWITWHGIALN
VNTDLSYFGLINACGITEFGVTSMKKLGIEVEIEEVMDRLVDKFEEVFEIKFEEIDLTRLAVVDSAKA

Sequences:

>Translated_228_residues
MRRGEVLKLGVVPYLEGKEIQLKAFEKVKKEETDGILILLQHKPVYTIGVSGGYDEDILVPIDYIKEKAELYKVERGGKI
TFHGPGQVVAYPIFNLAKWQKDVHLFVHNLEEAVIRLLREYGIIAGRKEKYTGVWVGDEKICAIGIAVKRWITWHGIALN
VNTDLSYFGLINACGITEFGVTSMKKLGIEVEIEEVMDRLVDKFEEVFEIKFEEIDLTRLAVVDSAKA
>Mature_228_residues
MRRGEVLKLGVVPYLEGKEIQLKAFEKVKKEETDGILILLQHKPVYTIGVSGGYDEDILVPIDYIKEKAELYKVERGGKI
TFHGPGQVVAYPIFNLAKWQKDVHLFVHNLEEAVIRLLREYGIIAGRKEKYTGVWVGDEKICAIGIAVKRWITWHGIALN
VNTDLSYFGLINACGITEFGVTSMKKLGIEVEIEEVMDRLVDKFEEVFEIKFEEIDLTRLAVVDSAKA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=223, Percent_Identity=35.8744394618834, Blast_Score=131, Evalue=5e-31,
Organism=Escherichia coli, GI87081767, Length=198, Percent_Identity=35.3535353535354, Blast_Score=121, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6323268, Length=183, Percent_Identity=33.879781420765, Blast_Score=101, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21355483, Length=218, Percent_Identity=38.9908256880734, Blast_Score=150, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_THETN (Q8R9E0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623270.1
- ProteinModelPortal:   Q8R9E0
- SMR:   Q8R9E0
- GeneID:   996237
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1673
- NMPDR:   fig|273068.3.peg.1653
- HOGENOM:   HBG716160
- OMA:   LCEPAGP
- ProtClustDB:   CLSK901366
- BioCyc:   TTEN273068:TTE1673-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25869; Mature: 25869

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRGEVLKLGVVPYLEGKEIQLKAFEKVKKEETDGILILLQHKPVYTIGVSGGYDEDILV
CCCCCEEEEECEECCCCCEEEHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCEEE
PIDYIKEKAELYKVERGGKITFHGPGQVVAYPIFNLAKWQKDVHLFVHNLEEAVIRLLRE
EHHHHHHHHHHEEECCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGIIAGRKEKYTGVWVGDEKICAIGIAVKRWITWHGIALNVNTDLSYFGLINACGITEFG
CCCCCCCCCCEEEEEECCCCEEEEHHHHHHEEEEEEEEEEECCCCHHHHHHHHCCCCHHC
VTSMKKLGIEVEIEEVMDRLVDKFEEVFEIKFEEIDLTRLAVVDSAKA
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHEEHHHCCEEEEEEECCCCC
>Mature Secondary Structure
MRRGEVLKLGVVPYLEGKEIQLKAFEKVKKEETDGILILLQHKPVYTIGVSGGYDEDILV
CCCCCEEEEECEECCCCCEEEHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCEEE
PIDYIKEKAELYKVERGGKITFHGPGQVVAYPIFNLAKWQKDVHLFVHNLEEAVIRLLRE
EHHHHHHHHHHEEECCCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGIIAGRKEKYTGVWVGDEKICAIGIAVKRWITWHGIALNVNTDLSYFGLINACGITEFG
CCCCCCCCCCEEEEEECCCCEEEEHHHHHHEEEEEEEEEEECCCCHHHHHHHHCCCCHHC
VTSMKKLGIEVEIEEVMDRLVDKFEEVFEIKFEEIDLTRLAVVDSAKA
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHEEHHHCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336