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The map label for this gene is LipA2

Identifier: 20808098

GI number: 20808098

Start: 1619625

End: 1620479

Strand: Reverse

Name: LipA2

Synonym: TTE1672

Alternate gene names: 20808098

Gene position: 1620479-1619625 (Counterclockwise)

Preceding gene: 20808099

Following gene: 20808097

Centisome position: 60.25

GC content: 41.52

Gene sequence:

>855_bases
TTGACAGTGCGAAAGCCTGAATGGCTTAAAGTGAGAATTTTAAGTGAGGATTTAAACAGGATGGAAGCTTTTTTGAAAAA
TATGGCCTTAAATACCGTATGTCAAAGTGCAAACTGCCCAAACATGGGGGAGTGCTTTGCAAGGAGAACAGCAACTTTTA
TGATAATGGGCAACATATGTACTAGAAACTGCAGATTTTGTGCAGTGGAAAAAGGGCATCCTCAGCCCCTTGATGAAGAA
GAGCCGAGAAGAGTGGCGGAAGCAGCTAGAAGGTTGGGGTTAAGGCATGTGGTAGTTACGTCTGTTACAAGGGATGATTT
GCCAGACGGTGGGGCTTCACATTTTGCGAAGACAATATATGAGCTAAAAAAGCTGCCAGGAGTCACTGTTGAAGTGCTTG
TGCCTGATTTTATGGGAAATGAAGAGGCAATCAGGACTGTTGTGGAAGCAAAACCTGATGTAATAAATCACAATGTAGAG
ACGGTTCCGAGGCTTTACTCAAGAGTGAGGCCAAAAGCGGATTACATTAGGTCTTTAAACCTCCTTAAAAAGGTCAAAGA
GCTGGACCCTCTAATACTTACTAAATCAGGGATTATGGTGGGACTGGGCGAAACAGAAGAGGAAGTAATAGAAGTTATGA
AGGATTTAAGAGATATTGATTGCGATATGATGACCATAGGACAGTATCTAAGACCTTCTCATAAGCACATAGAAGTAGCT
GAATATGTAACCCCTGAACAGTTTAAAAGATATGAAGAGATAGGCTACAAATTAGGATTTAAGCATGTAGCTTCTGGGCC
GTTGGTTAGAAGTTCTTATCATGCTGATGTGGGTTTGAGTTTAGCAAGAGGATGA

Upstream 100 bases:

>100_bases
GAAGTTGAAATAGAAGAAGTGATGGACAGACTTGTGGACAAGTTTGAAGAGGTGTTCGAAATAAAATTTGAAGAGATTGA
TTTAACTAGATTGGCGGTGG

Downstream 100 bases:

>100_bases
GTTATTTGTGGTAAAGAAAAAGCAGCTTTCAAAATGGGCTTTTAATGAAGGCTGCTTTTTTTGTAAAAAGGGGGATTTGA
ATGAAGGATTTTATTTCCTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTVRKPEWLKVRILSEDLNRMEAFLKNMALNTVCQSANCPNMGECFARRTATFMIMGNICTRNCRFCAVEKGHPQPLDEE
EPRRVAEAARRLGLRHVVVTSVTRDDLPDGGASHFAKTIYELKKLPGVTVEVLVPDFMGNEEAIRTVVEAKPDVINHNVE
TVPRLYSRVRPKADYIRSLNLLKKVKELDPLILTKSGIMVGLGETEEEVIEVMKDLRDIDCDMMTIGQYLRPSHKHIEVA
EYVTPEQFKRYEEIGYKLGFKHVASGPLVRSSYHADVGLSLARG

Sequences:

>Translated_284_residues
MTVRKPEWLKVRILSEDLNRMEAFLKNMALNTVCQSANCPNMGECFARRTATFMIMGNICTRNCRFCAVEKGHPQPLDEE
EPRRVAEAARRLGLRHVVVTSVTRDDLPDGGASHFAKTIYELKKLPGVTVEVLVPDFMGNEEAIRTVVEAKPDVINHNVE
TVPRLYSRVRPKADYIRSLNLLKKVKELDPLILTKSGIMVGLGETEEEVIEVMKDLRDIDCDMMTIGQYLRPSHKHIEVA
EYVTPEQFKRYEEIGYKLGFKHVASGPLVRSSYHADVGLSLARG
>Mature_283_residues
TVRKPEWLKVRILSEDLNRMEAFLKNMALNTVCQSANCPNMGECFARRTATFMIMGNICTRNCRFCAVEKGHPQPLDEEE
PRRVAEAARRLGLRHVVVTSVTRDDLPDGGASHFAKTIYELKKLPGVTVEVLVPDFMGNEEAIRTVVEAKPDVINHNVET
VPRLYSRVRPKADYIRSLNLLKKVKELDPLILTKSGIMVGLGETEEEVIEVMKDLRDIDCDMMTIGQYLRPSHKHIEVAE
YVTPEQFKRYEEIGYKLGFKHVASGPLVRSSYHADVGLSLARG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=50, Blast_Score=287, Evalue=8e-78,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=47.9674796747967, Blast_Score=235, Evalue=3e-62,
Organism=Escherichia coli, GI1786846, Length=279, Percent_Identity=50.179211469534, Blast_Score=290, Evalue=8e-80,
Organism=Caenorhabditis elegans, GI32564533, Length=285, Percent_Identity=46.6666666666667, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6324770, Length=279, Percent_Identity=45.5197132616487, Blast_Score=255, Evalue=5e-69,
Organism=Drosophila melanogaster, GI221513272, Length=282, Percent_Identity=48.2269503546099, Blast_Score=272, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_THETN (Q8R9E1)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623269.1
- ProteinModelPortal:   Q8R9E1
- GeneID:   996236
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1672
- NMPDR:   fig|273068.3.peg.1652
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   TTEN273068:TTE1672-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32155; Mature: 32024

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRKPEWLKVRILSEDLNRMEAFLKNMALNTVCQSANCPNMGECFARRTATFMIMGNIC
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCHH
TRNCRFCAVEKGHPQPLDEEEPRRVAEAARRLGLRHVVVTSVTRDDLPDGGASHFAKTIY
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHH
ELKKLPGVTVEVLVPDFMGNEEAIRTVVEAKPDVINHNVETVPRLYSRVRPKADYIRSLN
HHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHH
LLKKVKELDPLILTKSGIMVGLGETEEEVIEVMKDLRDIDCDMMTIGQYLRPSHKHIEVA
HHHHHHHCCCEEEECCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHH
EYVTPEQFKRYEEIGYKLGFKHVASGPLVRSSYHADVGLSLARG
HHCCHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure 
TVRKPEWLKVRILSEDLNRMEAFLKNMALNTVCQSANCPNMGECFARRTATFMIMGNIC
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCHH
TRNCRFCAVEKGHPQPLDEEEPRRVAEAARRLGLRHVVVTSVTRDDLPDGGASHFAKTIY
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHH
ELKKLPGVTVEVLVPDFMGNEEAIRTVVEAKPDVINHNVETVPRLYSRVRPKADYIRSLN
HHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHH
LLKKVKELDPLILTKSGIMVGLGETEEEVIEVMKDLRDIDCDMMTIGQYLRPSHKHIEVA
HHHHHHHCCCEEEECCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEHH
EYVTPEQFKRYEEIGYKLGFKHVASGPLVRSSYHADVGLSLARG
HHCCHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336