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The map label for this gene is MiaB3

Identifier: 20807824

GI number: 20807824

Start: 1359564

End: 1360874

Strand: Reverse

Name: MiaB3

Synonym: TTE1377

Alternate gene names: 20807824

Gene position: 1360874-1359564 (Counterclockwise)

Preceding gene: 20807825

Following gene: 20807823

Centisome position: 50.6

GC content: 34.78

Gene sequence:

>1311_bases
ATGAGAAACGTTGGAATTATCTCATTAGGGTGCCCGAAAAACAGCGTTGACTCAGAAAAAATGCTAGCTTTACTGAAAGA
AAAGGGGTACAATATAGTAAATGATGAAAAAAAGGCTGATGTCATAATAATAAATACTTGTGCCTTTATTGAAGATGCCA
AAAAAGAATCTATCGAATATATAATCCAAATGGGGGAGCTTAAGAAGAAGAGATTAAAATATCTAATTGCTACTGGATGC
CTCTCTGAAAGGTACAATAAAGAACTTATAAAAGAATTGCCTGAATTAGACGCAGTTATAGGAACAGGAGATTTTACGCA
AATTGTAGATGTGATTGAAGAAGTAAAAAAAGGAAAAAAAGTTTTAAAATATGGACATCCAGACCTTTTAAACGACGAAG
GAATCCCACGAATTCTTACCACTCCCCCTTATTACGCTTATTTGAAGATTGCAGAAGGCTGCAGCAACGCCTGCTCTTTC
TGCATAATTCCCAAACTAAGGGGAAAATACAAGAGCCGAAAAATGGAAAATATTATTGAAGAGGCTCAAGAACTTGCTAG
AAAAGGTGTTAAAGAGCTTATCATCATTGCACAGGATACAACAAAATATGGCATTGACCTTTACAAAAAGTTAATGCTTC
CTCAACTTTTAAGAGAATTATCTAAAATAGAAGAACTAAAATGGATTAGATTGCTTTATGCCTATCCTGATAGCGTGACT
GATGAATTGATAGAAGAGATAAAAAACAATCAAAAGATAGTAAAATATATAGACATCCCTTTGCAGCACTCTAGTGAATC
TGTATTAAAAAGAATGAACAGAGGTACAACAAGGCGTAAAATAGAAGAAGTCATATCAAAACTACGAAGTATACCAGGAA
TGGTTATGAGGACTACTTTTATGGTAGGATTTCCAGGAGAAACAGAGGAAGAGTTTGAAGACTTGAAAAACTTCATAAAG
GAAAAAAGGTTTGAGAGAGTAGGAGTTTTTACTTACTCTAGAGAAGAAGGAACCAAGTCCTATTACATGAAGCCACAGAT
AAGAAAAAAGGTTAAGTTAAAGCGACAAGAAGAACTTATGGAAATTCAAAAGCAAATTTCCTACGAATTCAATATGTCAA
AAATAGGAACTAAGTTAGAGGTTTTAATTGAGGGATTTGAAGATGGGGTGTATTACGGAAGAAGTTACATGGATGCTCCA
GAGATTGACGGTCTTGTATATGTGAGAAGCGACAAAAAACTCTTTCCAGGCGATTTTGTTACAGTAACAGTAGTAGATGC
GTTTGAATACGACCTTGTGGGGGAATATTGA

Upstream 100 bases:

>100_bases
TTGAGCAAAAAGGAATAATAAGCGGATACGACGGAGCAAAACCGAGGCAAATTATACTGCCCAGAGAAGAAATACAAAAG
ATTCTAGAAAGGAAGTCGGT

Downstream 100 bases:

>100_bases
TGAACCTAGCTAATAAACTGACTATGTCGCGACTTTTTTTAATACCCGTCTTTATGGTATTTATGCTGGTAAAAATTCCG
TATGGAGATTTCATAGCTGC

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRNVGIISLGCPKNSVDSEKMLALLKEKGYNIVNDEKKADVIIINTCAFIEDAKKESIEYIIQMGELKKKRLKYLIATGC
LSERYNKELIKELPELDAVIGTGDFTQIVDVIEEVKKGKKVLKYGHPDLLNDEGIPRILTTPPYYAYLKIAEGCSNACSF
CIIPKLRGKYKSRKMENIIEEAQELARKGVKELIIIAQDTTKYGIDLYKKLMLPQLLRELSKIEELKWIRLLYAYPDSVT
DELIEEIKNNQKIVKYIDIPLQHSSESVLKRMNRGTTRRKIEEVISKLRSIPGMVMRTTFMVGFPGETEEEFEDLKNFIK
EKRFERVGVFTYSREEGTKSYYMKPQIRKKVKLKRQEELMEIQKQISYEFNMSKIGTKLEVLIEGFEDGVYYGRSYMDAP
EIDGLVYVRSDKKLFPGDFVTVTVVDAFEYDLVGEY

Sequences:

>Translated_436_residues
MRNVGIISLGCPKNSVDSEKMLALLKEKGYNIVNDEKKADVIIINTCAFIEDAKKESIEYIIQMGELKKKRLKYLIATGC
LSERYNKELIKELPELDAVIGTGDFTQIVDVIEEVKKGKKVLKYGHPDLLNDEGIPRILTTPPYYAYLKIAEGCSNACSF
CIIPKLRGKYKSRKMENIIEEAQELARKGVKELIIIAQDTTKYGIDLYKKLMLPQLLRELSKIEELKWIRLLYAYPDSVT
DELIEEIKNNQKIVKYIDIPLQHSSESVLKRMNRGTTRRKIEEVISKLRSIPGMVMRTTFMVGFPGETEEEFEDLKNFIK
EKRFERVGVFTYSREEGTKSYYMKPQIRKKVKLKRQEELMEIQKQISYEFNMSKIGTKLEVLIEGFEDGVYYGRSYMDAP
EIDGLVYVRSDKKLFPGDFVTVTVVDAFEYDLVGEY
>Mature_436_residues
MRNVGIISLGCPKNSVDSEKMLALLKEKGYNIVNDEKKADVIIINTCAFIEDAKKESIEYIIQMGELKKKRLKYLIATGC
LSERYNKELIKELPELDAVIGTGDFTQIVDVIEEVKKGKKVLKYGHPDLLNDEGIPRILTTPPYYAYLKIAEGCSNACSF
CIIPKLRGKYKSRKMENIIEEAQELARKGVKELIIIAQDTTKYGIDLYKKLMLPQLLRELSKIEELKWIRLLYAYPDSVT
DELIEEIKNNQKIVKYIDIPLQHSSESVLKRMNRGTTRRKIEEVISKLRSIPGMVMRTTFMVGFPGETEEEFEDLKNFIK
EKRFERVGVFTYSREEGTKSYYMKPQIRKKVKLKRQEELMEIQKQISYEFNMSKIGTKLEVLIEGFEDGVYYGRSYMDAP
EIDGLVYVRSDKKLFPGDFVTVTVVDAFEYDLVGEY

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=27.8350515463918, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI28872784, Length=485, Percent_Identity=27.8350515463918, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI93277076, Length=411, Percent_Identity=27.4939172749392, Blast_Score=148, Evalue=8e-36,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=39.6860986547085, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1786882, Length=386, Percent_Identity=27.720207253886, Blast_Score=171, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=466, Percent_Identity=23.8197424892704, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71996771, Length=332, Percent_Identity=28.9156626506024, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=26.8085106382979, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI19922432, Length=461, Percent_Identity=27.9826464208243, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_THETN (Q8RA52)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622995.1
- ProteinModelPortal:   Q8RA52
- SMR:   Q8RA52
- GeneID:   995462
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1377
- NMPDR:   fig|273068.3.peg.1342
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK901201
- BioCyc:   TTEN273068:TTE1377-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50471; Mature: 50471

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVGIISLGCPKNSVDSEKMLALLKEKGYNIVNDEKKADVIIINTCAFIEDAKKESIEY
CCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
IIQMGELKKKRLKYLIATGCLSERYNKELIKELPELDAVIGTGDFTQIVDVIEEVKKGKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCHHHHHHHHHHHHHHHH
VLKYGHPDLLNDEGIPRILTTPPYYAYLKIAEGCSNACSFCIIPKLRGKYKSRKMENIIE
HHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCEEEEHHHHCHHHHHHHHHHHH
EAQELARKGVKELIIIAQDTTKYGIDLYKKLMLPQLLRELSKIEELKWIRLLYAYPDSVT
HHHHHHHCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
DELIEEIKNNQKIVKYIDIPLQHSSESVLKRMNRGTTRRKIEEVISKLRSIPGMVMRTTF
HHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHEE
MVGFPGETEEEFEDLKNFIKEKRFERVGVFTYSREEGTKSYYMKPQIRKKVKLKRQEELM
EEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEECHHHHHHHHHHHHHHHH
EIQKQISYEFNMSKIGTKLEVLIEGFEDGVYYGRSYMDAPEIDGLVYVRSDKKLFPGDFV
HHHHHHCCCCCHHHHCHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCEE
TVTVVDAFEYDLVGEY
EEEEEEHHCCCCCCCC
>Mature Secondary Structure
MRNVGIISLGCPKNSVDSEKMLALLKEKGYNIVNDEKKADVIIINTCAFIEDAKKESIEY
CCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
IIQMGELKKKRLKYLIATGCLSERYNKELIKELPELDAVIGTGDFTQIVDVIEEVKKGKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCHHHHHHHHHHHHHHHH
VLKYGHPDLLNDEGIPRILTTPPYYAYLKIAEGCSNACSFCIIPKLRGKYKSRKMENIIE
HHHCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCEEEEHHHHCHHHHHHHHHHHH
EAQELARKGVKELIIIAQDTTKYGIDLYKKLMLPQLLRELSKIEELKWIRLLYAYPDSVT
HHHHHHHCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
DELIEEIKNNQKIVKYIDIPLQHSSESVLKRMNRGTTRRKIEEVISKLRSIPGMVMRTTF
HHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHEE
MVGFPGETEEEFEDLKNFIKEKRFERVGVFTYSREEGTKSYYMKPQIRKKVKLKRQEELM
EEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEECHHHHHHHHHHHHHHHH
EIQKQISYEFNMSKIGTKLEVLIEGFEDGVYYGRSYMDAPEIDGLVYVRSDKKLFPGDFV
HHHHHHCCCCCHHHHCHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCEE
TVTVVDAFEYDLVGEY
EEEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336