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The map label for this gene is mnmE [H]

Identifier: 20807296

GI number: 20807296

Start: 813921

End: 815150

Strand: Direct

Name: mnmE [H]

Synonym: TTE0814

Alternate gene names: 20807296

Gene position: 813921-815150 (Clockwise)

Preceding gene: 20807295

Following gene: 20807297

Centisome position: 30.26

GC content: 39.84

Gene sequence:

>1230_bases
TTGAAAGGGCTGAAAAAGATGAACACAACACCGCGAAGCGAAAGGCTTCATATAGCAATATTTGGAAAAAGAAATGCTGG
AAAATCAAGTTTGATTAATGCAATAACAAATCAACCTATTGCGATTGTATCCCCAACGCCTGGCACGACAACCGATCCTG
TTTACAAATCAATGGAGATTTTACCATTGGGACCTGTTGTTTTGGTTGATACAGCTGGAATTGATGATGTGGGGGAGCTT
GGAAAACTCAGAGTTGAAAAAACACTTGAGGTTTTAAACAGGACAGACATTGCCATTTTGGTTGTCTCTGAAATAGACGA
TTTGAATTTTGAAAAGCAGCTTATAAAGCTTTTTAGAGAGAAAAAAGTGCCGAGCATTGGGGTTTTGAATAAGATAGACT
TAGACAAAGACTATAAAGAAAAACTCTCCTACCTGCAATCAACCTTAGGTATCAAGTTCTTACCAGTTTCATGTGCTACA
TTAAAAGGTATTGAGGAGCTTAAAAACGAGATTGCAAGGTCTGTGCCAGATGTTGGTGAGGATTTACGTATAGTGGGGGA
TTTGATAAACCCCGGTGACTTCGTTGTTTTGGTCGTACCAATCGACAAGGCTGCGCCAAAAGGAAGGCTGATTTTGCCTC
AGCAACAGACCATACGAGATATATTAGACAGCGATGCAATTGCAATTGTGACAAAGGAGTATGAGCTAAAAGAAACTTTG
GAGAATTTAGGCAAAAAACCCGCAATTGTGGTTACAGACTCACAGGCATTTTTGAAGGTTGATGCCGACACACCACCTGA
TATCCCTATGACATCATTTTCCATACTTTTTGCAAGGTATAAAGGCGACCTGTTTGAGTTTGTCGAGGGTGTAAAGAAGA
TAAAAAACCTAAAACCCTCCGATACGGTTTTGATTGCAGAAGCATGCACTCATCACAGACAATCGGATGATATTGGTACT
GTGAAAATCCCTCGCTGGATTAGACAAATTGCAGGTTTTGATATAAACTTTGAATGGGTATCGGGTTATGGCTATAAGAA
AGACCTTTCAAAATATGCCCTTATTGTTCACTGTGGGGGGTGCATGATAACAAGACGTGAAATGCTTTATAGAATAGAGC
TTGCAAAGAGCTATGGCGTACCAATTACAAACTATGGGATTATGATTGCGTATGTGCATGGCGTCCTGCCAAGGGCACTA
AAGCCTTTTGGAATTGAGTTTAAATATTAA

Upstream 100 bases:

>100_bases
AATAATTGAGGTGGTAAAGGAATTGAAGATCTTAAATGAGAATGAGGCTGAAGCACTACTAAATCCTTTTGAATTTGTAA
AGTTTAAAGAGTAAAAAATT

Downstream 100 bases:

>100_bases
ACTTGAATTGAGCAGGTAGCAAACAAATTAAAAGTTCAATTTCTATTATCTTGTTTCATTAACAATACGCCTATTAGATG
AATGCCAGGTTTTCGACTCG

Product: GTPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKGLKKMNTTPRSERLHIAIFGKRNAGKSSLINAITNQPIAIVSPTPGTTTDPVYKSMEILPLGPVVLVDTAGIDDVGEL
GKLRVEKTLEVLNRTDIAILVVSEIDDLNFEKQLIKLFREKKVPSIGVLNKIDLDKDYKEKLSYLQSTLGIKFLPVSCAT
LKGIEELKNEIARSVPDVGEDLRIVGDLINPGDFVVLVVPIDKAAPKGRLILPQQQTIRDILDSDAIAIVTKEYELKETL
ENLGKKPAIVVTDSQAFLKVDADTPPDIPMTSFSILFARYKGDLFEFVEGVKKIKNLKPSDTVLIAEACTHHRQSDDIGT
VKIPRWIRQIAGFDINFEWVSGYGYKKDLSKYALIVHCGGCMITRREMLYRIELAKSYGVPITNYGIMIAYVHGVLPRAL
KPFGIEFKY

Sequences:

>Translated_409_residues
MKGLKKMNTTPRSERLHIAIFGKRNAGKSSLINAITNQPIAIVSPTPGTTTDPVYKSMEILPLGPVVLVDTAGIDDVGEL
GKLRVEKTLEVLNRTDIAILVVSEIDDLNFEKQLIKLFREKKVPSIGVLNKIDLDKDYKEKLSYLQSTLGIKFLPVSCAT
LKGIEELKNEIARSVPDVGEDLRIVGDLINPGDFVVLVVPIDKAAPKGRLILPQQQTIRDILDSDAIAIVTKEYELKETL
ENLGKKPAIVVTDSQAFLKVDADTPPDIPMTSFSILFARYKGDLFEFVEGVKKIKNLKPSDTVLIAEACTHHRQSDDIGT
VKIPRWIRQIAGFDINFEWVSGYGYKKDLSKYALIVHCGGCMITRREMLYRIELAKSYGVPITNYGIMIAYVHGVLPRAL
KPFGIEFKY
>Mature_409_residues
MKGLKKMNTTPRSERLHIAIFGKRNAGKSSLINAITNQPIAIVSPTPGTTTDPVYKSMEILPLGPVVLVDTAGIDDVGEL
GKLRVEKTLEVLNRTDIAILVVSEIDDLNFEKQLIKLFREKKVPSIGVLNKIDLDKDYKEKLSYLQSTLGIKFLPVSCAT
LKGIEELKNEIARSVPDVGEDLRIVGDLINPGDFVVLVVPIDKAAPKGRLILPQQQTIRDILDSDAIAIVTKEYELKETL
ENLGKKPAIVVTDSQAFLKVDADTPPDIPMTSFSILFARYKGDLFEFVEGVKKIKNLKPSDTVLIAEACTHHRQSDDIGT
VKIPRWIRQIAGFDINFEWVSGYGYKKDLSKYALIVHCGGCMITRREMLYRIELAKSYGVPITNYGIMIAYVHGVLPRAL
KPFGIEFKY

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323665, Length=168, Percent_Identity=36.3095238095238, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 45539; Mature: 45539

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLKKMNTTPRSERLHIAIFGKRNAGKSSLINAITNQPIAIVSPTPGTTTDPVYKSMEI
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHCCEE
LPLGPVVLVDTAGIDDVGELGKLRVEKTLEVLNRTDIAILVVSEIDDLNFEKQLIKLFRE
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
KKVPSIGVLNKIDLDKDYKEKLSYLQSTLGIKFLPVSCATLKGIEELKNEIARSVPDVGE
HCCCCCCCCEECCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
DLRIVGDLINPGDFVVLVVPIDKAAPKGRLILPQQQTIRDILDSDAIAIVTKEYELKETL
CHHHHHHCCCCCCEEEEEEECCCCCCCCCEECCCHHHHHHHHCCCCEEEEECCHHHHHHH
ENLGKKPAIVVTDSQAFLKVDADTPPDIPMTSFSILFARYKGDLFEFVEGVKKIKNLKPS
HHHCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
DTVLIAEACTHHRQSDDIGTVKIPRWIRQIAGFDINFEWVSGYGYKKDLSKYALIVHCGG
CCEEEEHHHHCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHEEEEEECCC
CMITRREMLYRIELAKSYGVPITNYGIMIAYVHGVLPRALKPFGIEFKY
CEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MKGLKKMNTTPRSERLHIAIFGKRNAGKSSLINAITNQPIAIVSPTPGTTTDPVYKSMEI
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHCCEE
LPLGPVVLVDTAGIDDVGELGKLRVEKTLEVLNRTDIAILVVSEIDDLNFEKQLIKLFRE
EECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHH
KKVPSIGVLNKIDLDKDYKEKLSYLQSTLGIKFLPVSCATLKGIEELKNEIARSVPDVGE
HCCCCCCCCEECCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
DLRIVGDLINPGDFVVLVVPIDKAAPKGRLILPQQQTIRDILDSDAIAIVTKEYELKETL
CHHHHHHCCCCCCEEEEEEECCCCCCCCCEECCCHHHHHHHHCCCCEEEEECCHHHHHHH
ENLGKKPAIVVTDSQAFLKVDADTPPDIPMTSFSILFARYKGDLFEFVEGVKKIKNLKPS
HHHCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
DTVLIAEACTHHRQSDDIGTVKIPRWIRQIAGFDINFEWVSGYGYKKDLSKYALIVHCGG
CCEEEEHHHHCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCHHHHHHEEEEEECCC
CMITRREMLYRIELAKSYGVPITNYGIMIAYVHGVLPRALKPFGIEFKY
CEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA