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The map label for this gene is CarA

Identifier: 20807297

GI number: 20807297

Start: 816035

End: 817105

Strand: Direct

Name: CarA

Synonym: TTE0815

Alternate gene names: 20807297

Gene position: 816035-817105 (Clockwise)

Preceding gene: 20807296

Following gene: 20807298

Centisome position: 30.34

GC content: 36.13

Gene sequence:

>1071_bases
ATGAAAGGCTATTTGAAACTTGAGGATGGAAGTATATTTGAAGGTGAGTTAATAAGCAAAAACAAAAAAGGATATGGAGA
GGTTGTTTTTACCACAGGGATGACAGGCTATCAAGAAGCCATAACAGATCCTTCCTATGCAGGCCAAATAGTTGTCATGA
CATACCCTTTAATAGGGAATTACGGTATTAACAAATACGACTTTCAATCAGAAAAGCCTCATATAAGGGGATTTGTGGTA
CGGGAATATTGCGATAAGCCTAGCAATTTCCAAAGTGAGGAGTCGCTTTTGAGCTATCTTGATAACCACAACATACCAGT
ATTATCGGGAATTGATACAAGGGCTTTGACAAAAAAGCTTAGAGAAAATGGAACTATGAGAGGAATTATAACTTATAATC
CTGAGGACAATATAGAATTTGACCAGACAAATCTTTTGGAAGAAGTTTCTACGAAAAAGCCGTATCGTATAGCAGGTATT
GGTCCAAAACTTGCTTTTATTGACCTTGGTACAAAAAAGGGCATTTTAAAAATGCTAAATTCAGTTGGATTTGACATTTA
TGTGTTTCCTTACAATGCAAGTTATGATGATGTTATGCAAATAAATCCCGATGCAATATTTCTTTCAAATGGACCAGGAG
ACCCTAAAGATGCAGTTGATGCAATAGAACTTACTAAACACTTTATAGGTATGAAACCTGTGCTGGGGATATGCTTAGGG
CATCAAATAATAGCCCTTGCTCTTGGATGCAATACAGTAAAGATGAAATTTGGCCACAGAGGTGCAAATCAACCTGTTAA
AGATTTGCTGACAAATAAAGACTATATAACTTCTCAAAACCACGGATACGCTGTTGAAGAGGAGTCAATAGATAAAGACA
AGATAACTGTAACCCACATAAACTTAAACGATGGAACAGTAGAAGGCATTATGCATAAGTTTCTGCCAGTTTTTTCTGTA
CAGTATCATCCTGAGGCATGTCCTGGTCCTCGTGATTCAACAGATATTTTTGATAAATTTATGGATATAGTCATGGTTTA
CAAAAGGAGGTTTTATTTTGCCGAAGTATAA

Upstream 100 bases:

>100_bases
TTAATAAAAGAACTGCAAATAAATGTAGGAAAATTTAGAAACTTTATAAATACAGCGAGAATAATACTGTGGGATATACT
TTGTGAAGAGGTGTGTTAGT

Downstream 100 bases:

>100_bases
GGATATTAGTAAGGTTCTGGTAATAGGTTCGGGTCCGATTATAATAGGGCAAGCAGCGGAGTTTGATTATTCAGGAACCC
AAGCCTGCAAATCTTTAAAG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKGYLKLEDGSIFEGELISKNKKGYGEVVFTTGMTGYQEAITDPSYAGQIVVMTYPLIGNYGINKYDFQSEKPHIRGFVV
REYCDKPSNFQSEESLLSYLDNHNIPVLSGIDTRALTKKLRENGTMRGIITYNPEDNIEFDQTNLLEEVSTKKPYRIAGI
GPKLAFIDLGTKKGILKMLNSVGFDIYVFPYNASYDDVMQINPDAIFLSNGPGDPKDAVDAIELTKHFIGMKPVLGICLG
HQIIALALGCNTVKMKFGHRGANQPVKDLLTNKDYITSQNHGYAVEEESIDKDKITVTHINLNDGTVEGIMHKFLPVFSV
QYHPEACPGPRDSTDIFDKFMDIVMVYKRRFYFAEV

Sequences:

>Translated_356_residues
MKGYLKLEDGSIFEGELISKNKKGYGEVVFTTGMTGYQEAITDPSYAGQIVVMTYPLIGNYGINKYDFQSEKPHIRGFVV
REYCDKPSNFQSEESLLSYLDNHNIPVLSGIDTRALTKKLRENGTMRGIITYNPEDNIEFDQTNLLEEVSTKKPYRIAGI
GPKLAFIDLGTKKGILKMLNSVGFDIYVFPYNASYDDVMQINPDAIFLSNGPGDPKDAVDAIELTKHFIGMKPVLGICLG
HQIIALALGCNTVKMKFGHRGANQPVKDLLTNKDYITSQNHGYAVEEESIDKDKITVTHINLNDGTVEGIMHKFLPVFSV
QYHPEACPGPRDSTDIFDKFMDIVMVYKRRFYFAEV
>Mature_356_residues
MKGYLKLEDGSIFEGELISKNKKGYGEVVFTTGMTGYQEAITDPSYAGQIVVMTYPLIGNYGINKYDFQSEKPHIRGFVV
REYCDKPSNFQSEESLLSYLDNHNIPVLSGIDTRALTKKLRENGTMRGIITYNPEDNIEFDQTNLLEEVSTKKPYRIAGI
GPKLAFIDLGTKKGILKMLNSVGFDIYVFPYNASYDDVMQINPDAIFLSNGPGDPKDAVDAIELTKHFIGMKPVLGICLG
HQIIALALGCNTVKMKFGHRGANQPVKDLLTNKDYITSQNHGYAVEEESIDKDKITVTHINLNDGTVEGIMHKFLPVFSV
QYHPEACPGPRDSTDIFDKFMDIVMVYKRRFYFAEV

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=359, Percent_Identity=42.3398328690808, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI169790915, Length=360, Percent_Identity=36.1111111111111, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI21361331, Length=360, Percent_Identity=36.1111111111111, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=43.8502673796791, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1789760, Length=153, Percent_Identity=29.4117647058824, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=367, Percent_Identity=40.8719346049046, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6324878, Length=369, Percent_Identity=41.1924119241192, Blast_Score=276, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=36.9897959183673, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI45555749, Length=372, Percent_Identity=41.6666666666667, Blast_Score=264, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24642586, Length=372, Percent_Identity=41.6666666666667, Blast_Score=264, Evalue=6e-71,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_THETN (Q8RBK1)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622468.1
- ProteinModelPortal:   Q8RBK1
- SMR:   Q8RBK1
- GeneID:   996945
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0815
- NMPDR:   fig|273068.3.peg.996
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   TTEN273068:TTE0815-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39976; Mature: 39976

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 238-238 ACT_SITE 323-323 ACT_SITE 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGYLKLEDGSIFEGELISKNKKGYGEVVFTTGMTGYQEAITDPSYAGQIVVMTYPLIGN
CCCEEEECCCCEECCHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEEEEECEECC
YGINKYDFQSEKPHIRGFVVREYCDKPSNFQSEESLLSYLDNHNIPVLSGIDTRALTKKL
CCCCCCCCCCCCCCHHEEEEHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHH
RENGTMRGIITYNPEDNIEFDQTNLLEEVSTKKPYRIAGIGPKLAFIDLGTKKGILKMLN
HHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCHHHHHHHH
SVGFDIYVFPYNASYDDVMQINPDAIFLSNGPGDPKDAVDAIELTKHFIGMKPVLGICLG
HCCCEEEEEEECCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
HQIIALALGCNTVKMKFGHRGANQPVKDLLTNKDYITSQNHGYAVEEESIDKDKITVTHI
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHCCCCEEECCCCCEEECCCCCCCCCEEEEEE
NLNDGTVEGIMHKFLPVFSVQYHPEACPGPRDSTDIFDKFMDIVMVYKRRFYFAEV
ECCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MKGYLKLEDGSIFEGELISKNKKGYGEVVFTTGMTGYQEAITDPSYAGQIVVMTYPLIGN
CCCEEEECCCCEECCHHHCCCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEEEEECEECC
YGINKYDFQSEKPHIRGFVVREYCDKPSNFQSEESLLSYLDNHNIPVLSGIDTRALTKKL
CCCCCCCCCCCCCCHHEEEEHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHH
RENGTMRGIITYNPEDNIEFDQTNLLEEVSTKKPYRIAGIGPKLAFIDLGTKKGILKMLN
HHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEECCCCHHHHHHHH
SVGFDIYVFPYNASYDDVMQINPDAIFLSNGPGDPKDAVDAIELTKHFIGMKPVLGICLG
HCCCEEEEEEECCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
HQIIALALGCNTVKMKFGHRGANQPVKDLLTNKDYITSQNHGYAVEEESIDKDKITVTHI
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHCCCCEEECCCCCEEECCCCCCCCCEEEEEE
NLNDGTVEGIMHKFLPVFSVQYHPEACPGPRDSTDIFDKFMDIVMVYKRRFYFAEV
ECCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336