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The map label for this gene is gatB

Identifier: 20807103

GI number: 20807103

Start: 611505

End: 612932

Strand: Direct

Name: gatB

Synonym: TTE0608

Alternate gene names: 20807103

Gene position: 611505-612932 (Clockwise)

Preceding gene: 20807102

Following gene: 20807104

Centisome position: 22.74

GC content: 40.69

Gene sequence:

>1428_bases
ATGAAATACGAAGCAGTAATCGGATTAGAAGTTCATGCAGAGCTCTTGACAGACAGTAAGATATTTTGTGGATGCAGCAC
CAAATTTGGCAGTGAACCAAATACACAGGTGTGCCCTGTATGCCTTGGACTTCCGGGCACTTTGCCTGTGCTCAATAAAA
AAGTAGTAGAATATGCAGTGAGGGCAGGGCTGGCTTTGAACTGCACAATTGCTAATTTCAGCAAGATGGACAGAAAAAAT
TATTTTTATCCAGACCTTCCAAAAGCCTACCAGATTTCCCAGTACGACCTTCCCCTTTGTAGCAATGGGTACATAGAAAT
CGAGGTAGAAGGCGGCACAAAGAGGATAGGCATAAAGAGGATACACATTGAAGAGGATGCTGGGAAACTTTTACACGAAG
GAACTGATGGATCTTTGGTAGACTACAACAGAGCAGGTGTTCCTTTGATTGAGATAGTTTCTGAGCCGGATATTTCTACT
CCTGAAGAAGCATATCAGTACCTTGTTAAATTAAAGAGTATTCTAGAATATACTGAAGTTTCTGACTGCAAGATGCAGGA
AGGGTCTTTGAGAGTTGACACAAATGTCTCTGTAAGGCCTGTGGGTACTACTGAGCTTGGAACCAAGATTGAGCTTAAAA
ATTTAAATTCTTTTAAAGCCGTTCAAAAAGCGCTGGAATATGAGATAAAGAGACAGATAAAAGTCTTGGAAGAGGGCGGC
ACTATAGTTCAGGAGACTCGCAGATGGAATGAAGCAAAAGGCATTACTGAGCCGATGAGGACAAAGGAAGAAGCTCATGA
CTACAGGTATTTCCCAGAGCCTGACCTCGTTCCAATAATTGTGACAGAAGAATGGAAAGAAGAGATAAGAAAGACATTGC
CTGAGATGCCTGATGCTAAAAGAGAGCGTTTCATCACACAATACGGCTTACCTGAATATGATGCTAAAGTCATAACCTCT
TCAAAGAAGATGGCTGACTTCTTTGAAAAGTGTGCTTCAAATTATCACTCTCCAAAGATAGTGAGCAACTGGTTGATGGG
AGAATTTGCACGCCTTTTAAATGACACAGGTAAAGAGATAGATGAAGTGCCAATCACTCCAGATATGCTAATAGAGCTTT
TAAAACTTGTTGATGATAATGTTATAAGCGGTTCTATTGCGAAAACAGTGTTTGAGGAAATGTTTTTCACAGGTAAAAAT
CCGCAAATCATAGTGGAAGAGAAGGGTTTAAGGCAGATAGCCGACGAAGGTGAATTGAGGAGGATTGTCAGAAAAGTCAT
CGAAGAAAATCCAAAATCCGTAGAAGATTACAAGAAAGGAAAAGAGAAGGCATTGGGCTTTTTAGTAGGGCAAGTGATGA
AGGAGACGAAGGGGAAAGCAAATCCGCAGCTTACAAATCAACTTTTAAGAGAAGAACTTTCCAAATAG

Upstream 100 bases:

>100_bases
GCAAGCACTTTGATGAAGGCAGAATACTCAATGTGGCTTATGCTTTTGAAAAAGCGTACAAATTTGATGCCAAGCCACAG
GCCATAGGAGGTGAAAGATA

Downstream 100 bases:

>100_bases
CCTGCGCAAATTGCAGGCTATTTTTTTAAAAGACTGACAATTACAACTCAAGTTTACATTTTAAACAATTTTCATCGAAT
TCCTACAATACTCTTGCCAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKYEAVIGLEVHAELLTDSKIFCGCSTKFGSEPNTQVCPVCLGLPGTLPVLNKKVVEYAVRAGLALNCTIANFSKMDRKN
YFYPDLPKAYQISQYDLPLCSNGYIEIEVEGGTKRIGIKRIHIEEDAGKLLHEGTDGSLVDYNRAGVPLIEIVSEPDIST
PEEAYQYLVKLKSILEYTEVSDCKMQEGSLRVDTNVSVRPVGTTELGTKIELKNLNSFKAVQKALEYEIKRQIKVLEEGG
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTEEWKEEIRKTLPEMPDAKRERFITQYGLPEYDAKVITS
SKKMADFFEKCASNYHSPKIVSNWLMGEFARLLNDTGKEIDEVPITPDMLIELLKLVDDNVISGSIAKTVFEEMFFTGKN
PQIIVEEKGLRQIADEGELRRIVRKVIEENPKSVEDYKKGKEKALGFLVGQVMKETKGKANPQLTNQLLREELSK

Sequences:

>Translated_475_residues
MKYEAVIGLEVHAELLTDSKIFCGCSTKFGSEPNTQVCPVCLGLPGTLPVLNKKVVEYAVRAGLALNCTIANFSKMDRKN
YFYPDLPKAYQISQYDLPLCSNGYIEIEVEGGTKRIGIKRIHIEEDAGKLLHEGTDGSLVDYNRAGVPLIEIVSEPDIST
PEEAYQYLVKLKSILEYTEVSDCKMQEGSLRVDTNVSVRPVGTTELGTKIELKNLNSFKAVQKALEYEIKRQIKVLEEGG
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTEEWKEEIRKTLPEMPDAKRERFITQYGLPEYDAKVITS
SKKMADFFEKCASNYHSPKIVSNWLMGEFARLLNDTGKEIDEVPITPDMLIELLKLVDDNVISGSIAKTVFEEMFFTGKN
PQIIVEEKGLRQIADEGELRRIVRKVIEENPKSVEDYKKGKEKALGFLVGQVMKETKGKANPQLTNQLLREELSK
>Mature_475_residues
MKYEAVIGLEVHAELLTDSKIFCGCSTKFGSEPNTQVCPVCLGLPGTLPVLNKKVVEYAVRAGLALNCTIANFSKMDRKN
YFYPDLPKAYQISQYDLPLCSNGYIEIEVEGGTKRIGIKRIHIEEDAGKLLHEGTDGSLVDYNRAGVPLIEIVSEPDIST
PEEAYQYLVKLKSILEYTEVSDCKMQEGSLRVDTNVSVRPVGTTELGTKIELKNLNSFKAVQKALEYEIKRQIKVLEEGG
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTEEWKEEIRKTLPEMPDAKRERFITQYGLPEYDAKVITS
SKKMADFFEKCASNYHSPKIVSNWLMGEFARLLNDTGKEIDEVPITPDMLIELLKLVDDNVISGSIAKTVFEEMFFTGKN
PQIIVEEKGLRQIADEGELRRIVRKVIEENPKSVEDYKKGKEKALGFLVGQVMKETKGKANPQLTNQLLREELSK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=40.9274193548387, Blast_Score=352, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI115534094, Length=491, Percent_Identity=30.142566191446, Blast_Score=212, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=509, Percent_Identity=30.0589390962672, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=35.0202429149798, Blast_Score=295, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_THETN (Q8RC39)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622274.1
- ProteinModelPortal:   Q8RC39
- SMR:   Q8RC39
- GeneID:   996458
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0608
- NMPDR:   fig|273068.3.peg.154
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   TTEN273068:TTE0608-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53803; Mature: 53803

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYEAVIGLEVHAELLTDSKIFCGCSTKFGSEPNTQVCPVCLGLPGTLPVLNKKVVEYAV
CCCEEEEEHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
RAGLALNCTIANFSKMDRKNYFYPDLPKAYQISQYDLPLCSNGYIEIEVEGGTKRIGIKR
HCCCEEEEEEHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCEEEEEEE
IHIEEDAGKLLHEGTDGSLVDYNRAGVPLIEIVSEPDISTPEEAYQYLVKLKSILEYTEV
EEEECHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
SDCKMQEGSLRVDTNVSVRPVGTTELGTKIELKNLNSFKAVQKALEYEIKRQIKVLEEGG
CCCEECCCCEEECCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTEEWKEEIRKTLPEMPDAK
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCCHH
RERFITQYGLPEYDAKVITSSKKMADFFEKCASNYHSPKIVSNWLMGEFARLLNDTGKEI
HHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHH
DEVPITPDMLIELLKLVDDNVISGSIAKTVFEEMFFTGKNPQIIVEEKGLRQIADEGELR
HCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHH
RIVRKVIEENPKSVEDYKKGKEKALGFLVGQVMKETKGKANPQLTNQLLREELSK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYEAVIGLEVHAELLTDSKIFCGCSTKFGSEPNTQVCPVCLGLPGTLPVLNKKVVEYAV
CCCEEEEEHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
RAGLALNCTIANFSKMDRKNYFYPDLPKAYQISQYDLPLCSNGYIEIEVEGGTKRIGIKR
HCCCEEEEEEHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCCEEEEEEE
IHIEEDAGKLLHEGTDGSLVDYNRAGVPLIEIVSEPDISTPEEAYQYLVKLKSILEYTEV
EEEECHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
SDCKMQEGSLRVDTNVSVRPVGTTELGTKIELKNLNSFKAVQKALEYEIKRQIKVLEEGG
CCCEECCCCEEECCCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
TIVQETRRWNEAKGITEPMRTKEEAHDYRYFPEPDLVPIIVTEEWKEEIRKTLPEMPDAK
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHCCCCCCHH
RERFITQYGLPEYDAKVITSSKKMADFFEKCASNYHSPKIVSNWLMGEFARLLNDTGKEI
HHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHH
DEVPITPDMLIELLKLVDDNVISGSIAKTVFEEMFFTGKNPQIIVEEKGLRQIADEGELR
HCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHH
RIVRKVIEENPKSVEDYKKGKEKALGFLVGQVMKETKGKANPQLTNQLLREELSK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336