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The map label for this gene is RbsB3 [H]

Identifier: 20806810

GI number: 20806810

Start: 303390

End: 304472

Strand: Direct

Name: RbsB3 [H]

Synonym: TTE0289

Alternate gene names: 20806810

Gene position: 303390-304472 (Clockwise)

Preceding gene: 20806809

Following gene: 20806811

Centisome position: 11.28

GC content: 39.06

Gene sequence:

>1083_bases
ATGAAAGTGGGGGAAATGGTGAAGTTAGTAATAGCAGTGATTTTAACGATTATAGCGGTATTTCTGGGATTTTTAACTGT
CAAACAAGTAGTTGCCTACCACAGTGCGAAAAATTTATATAGAGAAGAGGATAAGAGTAAAAAGGTAAAAATAGGCTTTT
CTTTGGGTACTTTAAAAGAGGAAAGATGGGTTAAAGACAGGGATATTGTAATGGCTAAGTTAAAAGAACTTGGAGCAGAG
GTATTGGTTCAAAATGCCAATAATGATGATGAAGACCAGCTAAAACAAGTAAAATACCTTTTGGAGCAAAAGATTGACGT
TTTAATAATTGTTCCAAATGACCTGGAGAAAGCTTCATATGCTGTATCCCTAGCTCAGAAAGAAGGAGTAAAGGTTATAT
CTTACGATAGACTGGTTACAAGGTCTAATGTGGACCTTTATATCTCCTTTGACAATGTAAAAGTGGGGAAGTTTATGGCA
GAGTATTTAGTGAAAAGAGTTCCTAAGGGTAATTACCTTATTGTAAATGGGGCTACTACGGACAATAATACAAAGATGAT
AAAAGAAGGTTATGACAGCGTATTAAAACCCTTTATAGATAGGGGAGACATAAAAATTGTGAAAGAGGACTGGGCACCCA
ACTGGATGGCAGAGTATGCTTTTAATGTGACAGATGAAGTGCTGCAGAAGGGGATAAAAGTAGATGCCATTATTGCAGGG
GATGATGCGCTGGCAGGTGGGATTATAGAGGCTTTAGCTTTACACAGGCTGGCTGGGAAAATTCCGGTAGTGGGTCAGGA
TGCGGACCTCGCAGCATGTCAGAGAATAGTTGAAGGCACACAAGCTATGACTGTGTACAAGCCAATTGACAAGCTTGCAG
AGGCTACGGCTAGAATGGCAATGAAGCTTGCCAGAGGGGAGAAGCTGGATGTCAAAAATACCATATATGACGGCAAGTAT
TACGTTCCTTATTATGTTATTGAACCTATTCCAGTTGACAGGTCAAATCTTGATGACACTGTAATAAAAGATGGCTTTCA
TACAAGAGATCAGGTGTACAGAAATATAAGCGTTTCTAGGTAA

Upstream 100 bases:

>100_bases
GAGTTACAAAGTAGGATACAGTGACCCAAATTATTTCAGCAAAATCTTTAAGAAGGTCACAGGGGTAACGCCCACAGAGT
TCAAAGAGGGGGTGTAGGAA

Downstream 100 bases:

>100_bases
TAAAGGCTCATGGCCTGTATTTTTTTGTCTAAAAAAATTACAGCTAAGGTAAAAGTGAAAGGACAAGAAAAATAAAAAAG
TAAAGGATAACAAAAAAACT

Product: periplasmic sugar-binding proteins

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKVGEMVKLVIAVILTIIAVFLGFLTVKQVVAYHSAKNLYREEDKSKKVKIGFSLGTLKEERWVKDRDIVMAKLKELGAE
VLVQNANNDDEDQLKQVKYLLEQKIDVLIIVPNDLEKASYAVSLAQKEGVKVISYDRLVTRSNVDLYISFDNVKVGKFMA
EYLVKRVPKGNYLIVNGATTDNNTKMIKEGYDSVLKPFIDRGDIKIVKEDWAPNWMAEYAFNVTDEVLQKGIKVDAIIAG
DDALAGGIIEALALHRLAGKIPVVGQDADLAACQRIVEGTQAMTVYKPIDKLAEATARMAMKLARGEKLDVKNTIYDGKY
YVPYYVIEPIPVDRSNLDDTVIKDGFHTRDQVYRNISVSR

Sequences:

>Translated_360_residues
MKVGEMVKLVIAVILTIIAVFLGFLTVKQVVAYHSAKNLYREEDKSKKVKIGFSLGTLKEERWVKDRDIVMAKLKELGAE
VLVQNANNDDEDQLKQVKYLLEQKIDVLIIVPNDLEKASYAVSLAQKEGVKVISYDRLVTRSNVDLYISFDNVKVGKFMA
EYLVKRVPKGNYLIVNGATTDNNTKMIKEGYDSVLKPFIDRGDIKIVKEDWAPNWMAEYAFNVTDEVLQKGIKVDAIIAG
DDALAGGIIEALALHRLAGKIPVVGQDADLAACQRIVEGTQAMTVYKPIDKLAEATARMAMKLARGEKLDVKNTIYDGKY
YVPYYVIEPIPVDRSNLDDTVIKDGFHTRDQVYRNISVSR
>Mature_360_residues
MKVGEMVKLVIAVILTIIAVFLGFLTVKQVVAYHSAKNLYREEDKSKKVKIGFSLGTLKEERWVKDRDIVMAKLKELGAE
VLVQNANNDDEDQLKQVKYLLEQKIDVLIIVPNDLEKASYAVSLAQKEGVKVISYDRLVTRSNVDLYISFDNVKVGKFMA
EYLVKRVPKGNYLIVNGATTDNNTKMIKEGYDSVLKPFIDRGDIKIVKEDWAPNWMAEYAFNVTDEVLQKGIKVDAIIAG
DDALAGGIIEALALHRLAGKIPVVGQDADLAACQRIVEGTQAMTVYKPIDKLAEATARMAMKLARGEKLDVKNTIYDGKY
YVPYYVIEPIPVDRSNLDDTVIKDGFHTRDQVYRNISVSR

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=308, Percent_Identity=44.1558441558442, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI1790192, Length=269, Percent_Identity=25.2788104089219, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40397; Mature: 40397

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGEMVKLVIAVILTIIAVFLGFLTVKQVVAYHSAKNLYREEDKSKKVKIGFSLGTLKE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
ERWVKDRDIVMAKLKELGAEVLVQNANNDDEDQLKQVKYLLEQKIDVLIIVPNDLEKASY
HHHCCHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
AVSLAQKEGVKVISYDRLVTRSNVDLYISFDNVKVGKFMAEYLVKRVPKGNYLIVNGATT
HHHHHHHCCCEEEEECCEEEECCCEEEEEECCEEHHHHHHHHHHHHCCCCCEEEEECCCC
DNNTKMIKEGYDSVLKPFIDRGDIKIVKEDWAPNWMAEYAFNVTDEVLQKGIKVDAIIAG
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHCCCCEEEEEEC
DDALAGGIIEALALHRLAGKIPVVGQDADLAACQRIVEGTQAMTVYKPIDKLAEATARMA
CCHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHH
MKLARGEKLDVKNTIYDGKYYVPYYVIEPIPVDRSNLDDTVIKDGFHTRDQVYRNISVSR
HHHHCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MKVGEMVKLVIAVILTIIAVFLGFLTVKQVVAYHSAKNLYREEDKSKKVKIGFSLGTLKE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
ERWVKDRDIVMAKLKELGAEVLVQNANNDDEDQLKQVKYLLEQKIDVLIIVPNDLEKASY
HHHCCHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
AVSLAQKEGVKVISYDRLVTRSNVDLYISFDNVKVGKFMAEYLVKRVPKGNYLIVNGATT
HHHHHHHCCCEEEEECCEEEECCCEEEEEECCEEHHHHHHHHHHHHCCCCCEEEEECCCC
DNNTKMIKEGYDSVLKPFIDRGDIKIVKEDWAPNWMAEYAFNVTDEVLQKGIKVDAIIAG
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHCCCCEEEEEEC
DDALAGGIIEALALHRLAGKIPVVGQDADLAACQRIVEGTQAMTVYKPIDKLAEATARMA
CCHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHH
MKLARGEKLDVKNTIYDGKYYVPYYVIEPIPVDRSNLDDTVIKDGFHTRDQVYRNISVSR
HHHHCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]