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The map label for this gene is CheY2 [H]

Identifier: 20806809

GI number: 20806809

Start: 301797

End: 303386

Strand: Direct

Name: CheY2 [H]

Synonym: TTE0288

Alternate gene names: 20806809

Gene position: 301797-303386 (Clockwise)

Preceding gene: 20806808

Following gene: 20806810

Centisome position: 11.22

GC content: 32.7

Gene sequence:

>1590_bases
ATGGTCAAGTTGTTAATAGCTGACGATGAGCAGATAGTCCTTGATTCTATTAAGTTTATAATAGAAAAAAACAATGTACA
GGCTGAAATTGTGGGCACAGCTAAGTCTGGCAGGGAAGCTATAGAGAAGGCAGATTTGTTAAAGCCGGACCTTATTTTTA
TGGACATAAGAATGCCTGGAATTGATGGAATAGAAGCGATAAGGCAGATTAAAGAAAGGCACAAAGAGATGATTTTTGTG
ATTATTACAGCTTACGAATATTTTAATTATGCAAAAGAGGCCATTGATTTAGGAGTTATGGATTACCTCTTAAAACCACT
GAATAAAAACAAAATAATAGAAGTTATTGAAAAGGCTTCAAAGGTCATAGAGGAAAGAAGGAAAGCAGTACTTACGGAAA
TAGAACTTAGAGAGAAAATCAATAAAATTATGCCTTATCTGGAAGAACAGTTTTTGTACACGGTGCTATTTGGAGGATAT
GTCGGAGGTAGTTTAGATTTTTATGAAGAAATCTTTGATATGAAGCTGGAAAAGGGATATATTATGATATTTCTCATGAG
ATCTGATACTAGTAATGAAAGAGAAAAGAGCATAGAAGTAAACCTTAAAAGGCAAAGCCTGTATGAGCATTTGAGAATTT
TTTTAAAAGGGGTAAGTAAATGCATATTGGGGCCCTTTTTTATGGATAAAATCTGTGTTTATTTTCCTGTAGAAGAGAAA
ACTCCTTTTGAGGTCAAAAACTCTTCTGTGAAAATAGCCAATAAAGTTCTGGAAAGTTTACAAGGGTCAGAAAAAGAAAA
TTTAAGAGTAGGAATCGGCAGAGAGTATAGCTTCAATAACATAGTGAAATCTTACTATGAAGCAGAGATGGCTTTAAAAC
TTTTTCCCGATGATACAATTGTCCATTTTGATGATGCAGTTGGCACTTCTACTATATCGCACTTTCCTTATCCTCTGGAT
AAAGAAAAATTATTTATTGAGAGACTTGTGGAAGGCGATTTAGCTAGAGCTTTTAAAGTTTTTGATGAGATTTATGAATG
GATGATTTTAGAATATGATAAAGATGCAGGAAAAATTAAGTCTAAATTGATAGAGCTCGTGGGAATAATAAGAAGGAGTT
TCTACTATTACCTTGAAGAAGGAAGTTTTAAAGAAGAAATAGCACAAATTGAGGAATTGCTGAAAATAGAGGATTTGAGA
GAATTAAAGCTTGTTTTTATAAAAATGTTAAAAGACATTGTGGAAAATATAAGAGTAGTTAACAAAAAGAAATGGGATAA
TATTACAATGAAAGTAATAAATTTTCTTGAAGAAAATTTTAGCAAAGACATAAGTTTGGAAGATGCTGCTAGAGAAGTAA
ACATGAGCTATCACTATTTTAGCAAGTTTTTTAAAGAACAGGTAGGGGAAAATTTTGTGGATTACCTTACAAATTTGAGA
ATACGAAAGGCAAAAGAACTTTTAAAAAATTCAAGCCTAAGTGTAAAAGAAGTGAGTTACAAAGTAGGATACAGTGACCC
AAATTATTTCAGCAAAATCTTTAAGAAGGTCACAGGGGTAACGCCCACAGAGTTCAAAGAGGGGGTGTAG

Upstream 100 bases:

>100_bases
GACATATCTAGTGTAGAAGATGTTATCGAGATAAAAAGTGAGATAGGGAAAGGCACCAAAGTCACTTTAAAAATTCCTGA
TAAAAAGGGTGAAAGGAATT

Downstream 100 bases:

>100_bases
GAAATGAAAGTGGGGGAAATGGTGAAGTTAGTAATAGCAGTGATTTTAACGATTATAGCGGTATTTCTGGGATTTTTAAC
TGTCAAACAAGTAGTTGCCT

Product: CheY-like domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MVKLLIADDEQIVLDSIKFIIEKNNVQAEIVGTAKSGREAIEKADLLKPDLIFMDIRMPGIDGIEAIRQIKERHKEMIFV
IITAYEYFNYAKEAIDLGVMDYLLKPLNKNKIIEVIEKASKVIEERRKAVLTEIELREKINKIMPYLEEQFLYTVLFGGY
VGGSLDFYEEIFDMKLEKGYIMIFLMRSDTSNEREKSIEVNLKRQSLYEHLRIFLKGVSKCILGPFFMDKICVYFPVEEK
TPFEVKNSSVKIANKVLESLQGSEKENLRVGIGREYSFNNIVKSYYEAEMALKLFPDDTIVHFDDAVGTSTISHFPYPLD
KEKLFIERLVEGDLARAFKVFDEIYEWMILEYDKDAGKIKSKLIELVGIIRRSFYYYLEEGSFKEEIAQIEELLKIEDLR
ELKLVFIKMLKDIVENIRVVNKKKWDNITMKVINFLEENFSKDISLEDAAREVNMSYHYFSKFFKEQVGENFVDYLTNLR
IRKAKELLKNSSLSVKEVSYKVGYSDPNYFSKIFKKVTGVTPTEFKEGV

Sequences:

>Translated_529_residues
MVKLLIADDEQIVLDSIKFIIEKNNVQAEIVGTAKSGREAIEKADLLKPDLIFMDIRMPGIDGIEAIRQIKERHKEMIFV
IITAYEYFNYAKEAIDLGVMDYLLKPLNKNKIIEVIEKASKVIEERRKAVLTEIELREKINKIMPYLEEQFLYTVLFGGY
VGGSLDFYEEIFDMKLEKGYIMIFLMRSDTSNEREKSIEVNLKRQSLYEHLRIFLKGVSKCILGPFFMDKICVYFPVEEK
TPFEVKNSSVKIANKVLESLQGSEKENLRVGIGREYSFNNIVKSYYEAEMALKLFPDDTIVHFDDAVGTSTISHFPYPLD
KEKLFIERLVEGDLARAFKVFDEIYEWMILEYDKDAGKIKSKLIELVGIIRRSFYYYLEEGSFKEEIAQIEELLKIEDLR
ELKLVFIKMLKDIVENIRVVNKKKWDNITMKVINFLEENFSKDISLEDAAREVNMSYHYFSKFFKEQVGENFVDYLTNLR
IRKAKELLKNSSLSVKEVSYKVGYSDPNYFSKIFKKVTGVTPTEFKEGV
>Mature_529_residues
MVKLLIADDEQIVLDSIKFIIEKNNVQAEIVGTAKSGREAIEKADLLKPDLIFMDIRMPGIDGIEAIRQIKERHKEMIFV
IITAYEYFNYAKEAIDLGVMDYLLKPLNKNKIIEVIEKASKVIEERRKAVLTEIELREKINKIMPYLEEQFLYTVLFGGY
VGGSLDFYEEIFDMKLEKGYIMIFLMRSDTSNEREKSIEVNLKRQSLYEHLRIFLKGVSKCILGPFFMDKICVYFPVEEK
TPFEVKNSSVKIANKVLESLQGSEKENLRVGIGREYSFNNIVKSYYEAEMALKLFPDDTIVHFDDAVGTSTISHFPYPLD
KEKLFIERLVEGDLARAFKVFDEIYEWMILEYDKDAGKIKSKLIELVGIIRRSFYYYLEEGSFKEEIAQIEELLKIEDLR
ELKLVFIKMLKDIVENIRVVNKKKWDNITMKVINFLEENFSKDISLEDAAREVNMSYHYFSKFFKEQVGENFVDYLTNLR
IRKAKELLKNSSLSVKEVSYKVGYSDPNYFSKIFKKVTGVTPTEFKEGV

Specific function: Member of the two-component regulatory system yesM/yesN [H]

COG id: COG4753

COG function: function code T; Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=31.4516129032258, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788725, Length=128, Percent_Identity=24.21875, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082052, Length=154, Percent_Identity=29.8701298701299, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790339, Length=97, Percent_Identity=29.8969072164948, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786251, Length=143, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788712, Length=115, Percent_Identity=29.5652173913043, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788550, Length=118, Percent_Identity=29.6610169491525, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790391, Length=93, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=89, Percent_Identity=38.2022471910112, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082197, Length=108, Percent_Identity=28.7037037037037, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788724, Length=128, Percent_Identity=28.90625, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018060
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00165 HTH_AraC; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 61860; Mature: 61860

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLLIADDEQIVLDSIKFIIEKNNVQAEIVGTAKSGREAIEKADLLKPDLIFMDIRMPG
CEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEEECCC
IDGIEAIRQIKERHKEMIFVIITAYEYFNYAKEAIDLGVMDYLLKPLNKNKIIEVIEKAS
CCHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KVIEERRKAVLTEIELREKINKIMPYLEEQFLYTVLFGGYVGGSLDFYEEIFDMKLEKGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IMIFLMRSDTSNEREKSIEVNLKRQSLYEHLRIFLKGVSKCILGPFFMDKICVYFPVEEK
EEEEEEECCCCCCHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
TPFEVKNSSVKIANKVLESLQGSEKENLRVGIGREYSFNNIVKSYYEAEMALKLFPDDTI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHEEECCCCCE
VHFDDAVGTSTISHFPYPLDKEKLFIERLVEGDLARAFKVFDEIYEWMILEYDKDAGKIK
EEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHH
SKLIELVGIIRRSFYYYLEEGSFKEEIAQIEELLKIEDLRELKLVFIKMLKDIVENIRVV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKKKWDNITMKVINFLEENFSKDISLEDAAREVNMSYHYFSKFFKEQVGENFVDYLTNLR
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCH
IRKAKELLKNSSLSVKEVSYKVGYSDPNYFSKIFKKVTGVTPTEFKEGV
HHHHHHHHHCCCCCHHHEEEEECCCCHHHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MVKLLIADDEQIVLDSIKFIIEKNNVQAEIVGTAKSGREAIEKADLLKPDLIFMDIRMPG
CEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEEECCC
IDGIEAIRQIKERHKEMIFVIITAYEYFNYAKEAIDLGVMDYLLKPLNKNKIIEVIEKAS
CCHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KVIEERRKAVLTEIELREKINKIMPYLEEQFLYTVLFGGYVGGSLDFYEEIFDMKLEKGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCE
IMIFLMRSDTSNEREKSIEVNLKRQSLYEHLRIFLKGVSKCILGPFFMDKICVYFPVEEK
EEEEEEECCCCCCHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
TPFEVKNSSVKIANKVLESLQGSEKENLRVGIGREYSFNNIVKSYYEAEMALKLFPDDTI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHEEECCCCCE
VHFDDAVGTSTISHFPYPLDKEKLFIERLVEGDLARAFKVFDEIYEWMILEYDKDAGKIK
EEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHH
SKLIELVGIIRRSFYYYLEEGSFKEEIAQIEELLKIEDLRELKLVFIKMLKDIVENIRVV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKKKWDNITMKVINFLEENFSKDISLEDAAREVNMSYHYFSKFFKEQVGENFVDYLTNLR
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCH
IRKAKELLKNSSLSVKEVSYKVGYSDPNYFSKIFKKVTGVTPTEFKEGV
HHHHHHHHHCCCCCHHHEEEEECCCCHHHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]