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The map label for this gene is RbsB4 [H]

Identifier: 20806811

GI number: 20806811

Start: 304646

End: 305782

Strand: Direct

Name: RbsB4 [H]

Synonym: TTE0290

Alternate gene names: 20806811

Gene position: 304646-305782 (Clockwise)

Preceding gene: 20806810

Following gene: 20806812

Centisome position: 11.33

GC content: 42.39

Gene sequence:

>1137_bases
ATGTCTAGGAAAGGTAAAATTTTTCTGGTTTTAACAGCCCTACTACTTGTAGTCAGTGCATTTTTGGGAGGGTGTTCCTC
TTCAAAGCAACCTGCGTCTAGCTCTAACTCTTCAGGAAAGCAATTAGCGATTGGGATAGTATTGCCTACAAAAGACGAGC
CGAGGTGGATTCAGGATGAGACTAGATTCCAAGATGCTTTGAAAGATACTCCGTATGCTGCAGAGATACTTTTCAGCCAG
GGAGACCCTGCTAAAGAAAAAGCAAATGTAGAGACGCTTTTGAGCAAGGGCATTAAAGTTCTCATTCTGTGCCCACACGA
TGCTAATGCTGCTGCAGCATCAGCAGAAGCAGCTAAAAAGGCTGGAGTAAAAGTGATTTCTTATGACCGCTTGATACTTA
ATACCGATGCTGTAGACTATTATGTCACTTTTGACAGCGTTCAGGTAGGGCAGCAACAGGGACAGTATCTGGTGGACCAT
GCGACTGGCAAAGGGAATCCACTTTACCTTTATGCAGGAGCTTTATCTGACAACAACTCCTTCTTGTTCTTCCAGGGTGC
ATGGGAAGTTTTGCAGCCTAAGATTGCCGATGGCACTTTTGTGATTGCAAATTCTGACAAGGCAAATGCTCTCAAAGATA
AAAAAGATTTGACTCGTCAGGAAATGGCAGATATAATAGGTCAAATTACAACAAACTGGGATTTCAATACGGCAAAGAAC
TTGGCTCAAGCTAATTTGGCAAAAGCACCTAAAGATCTCAAAGGAAATGTATTCATCCTTGCTCCGAATGACGGAACTGC
TAGGTCTATCGCAGATACTTTCGCAGCAGATCCAGATATTAAAAGCTATGTGATTACAGGGCAGGACGCAGAAAAGCCTT
CTATCCAGTATATAATCGATGGTAAGCAGTCTATGACTGTTTTCAAAGATGTGAGAATACTGGTTAAAGACGCTATAAAT
GTGGCTCTTACTTACCTCAAAGGCGGCACACCTGAATCTACGAAGACTTACAACAACGGTGTAAAGGATGTTCCATCTAA
GCCGTCACCTGTCCAAGTGGTGGATAAGAACAATGTAAAGCAGGTTCTCATAGACTCTGGCTACTATAAAGAGAGCGACT
TTAAATGGTCTAAATAA

Upstream 100 bases:

>100_bases
AAAAAAGTAAAGGATAACAAAAAAACTTTCAGAGGTGATTTTAAAGGAGAAGTAGAATTATATAAATGCAGTAAAACTTA
AAAAAGGAGAGGGTGTAAGG

Downstream 100 bases:

>100_bases
TTGAGGAGGTGTAAAAATGGGTGCCCCGCTTATAGAGCTTGTAAACGTCAATAAAAGTTTCTCTGGTGTGCAGGTGTTGT
TCAATGTCAATTTTAGGGTA

Product: periplasmic sugar-binding proteins

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSRKGKIFLVLTALLLVVSAFLGGCSSSKQPASSSNSSGKQLAIGIVLPTKDEPRWIQDETRFQDALKDTPYAAEILFSQ
GDPAKEKANVETLLSKGIKVLILCPHDANAAAASAEAAKKAGVKVISYDRLILNTDAVDYYVTFDSVQVGQQQGQYLVDH
ATGKGNPLYLYAGALSDNNSFLFFQGAWEVLQPKIADGTFVIANSDKANALKDKKDLTRQEMADIIGQITTNWDFNTAKN
LAQANLAKAPKDLKGNVFILAPNDGTARSIADTFAADPDIKSYVITGQDAEKPSIQYIIDGKQSMTVFKDVRILVKDAIN
VALTYLKGGTPESTKTYNNGVKDVPSKPSPVQVVDKNNVKQVLIDSGYYKESDFKWSK

Sequences:

>Translated_378_residues
MSRKGKIFLVLTALLLVVSAFLGGCSSSKQPASSSNSSGKQLAIGIVLPTKDEPRWIQDETRFQDALKDTPYAAEILFSQ
GDPAKEKANVETLLSKGIKVLILCPHDANAAAASAEAAKKAGVKVISYDRLILNTDAVDYYVTFDSVQVGQQQGQYLVDH
ATGKGNPLYLYAGALSDNNSFLFFQGAWEVLQPKIADGTFVIANSDKANALKDKKDLTRQEMADIIGQITTNWDFNTAKN
LAQANLAKAPKDLKGNVFILAPNDGTARSIADTFAADPDIKSYVITGQDAEKPSIQYIIDGKQSMTVFKDVRILVKDAIN
VALTYLKGGTPESTKTYNNGVKDVPSKPSPVQVVDKNNVKQVLIDSGYYKESDFKWSK
>Mature_377_residues
SRKGKIFLVLTALLLVVSAFLGGCSSSKQPASSSNSSGKQLAIGIVLPTKDEPRWIQDETRFQDALKDTPYAAEILFSQG
DPAKEKANVETLLSKGIKVLILCPHDANAAAASAEAAKKAGVKVISYDRLILNTDAVDYYVTFDSVQVGQQQGQYLVDHA
TGKGNPLYLYAGALSDNNSFLFFQGAWEVLQPKIADGTFVIANSDKANALKDKKDLTRQEMADIIGQITTNWDFNTAKNL
AQANLAKAPKDLKGNVFILAPNDGTARSIADTFAADPDIKSYVITGQDAEKPSIQYIIDGKQSMTVFKDVRILVKDAINV
ALTYLKGGTPESTKTYNNGVKDVPSKPSPVQVVDKNNVKQVLIDSGYYKESDFKWSK

Specific function: Required for effective transcriptional induction of the vir genes by monosaccharides in response to plant signals and for normal growth and chemotaxis towards certain sugars. Function as a periplasmic multiple sugar-binding receptor protein. It does not i

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=339, Percent_Identity=28.023598820059, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40999; Mature: 40868

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKGKIFLVLTALLLVVSAFLGGCSSSKQPASSSNSSGKQLAIGIVLPTKDEPRWIQDE
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHH
TRFQDALKDTPYAAEILFSQGDPAKEKANVETLLSKGIKVLILCPHDANAAAASAEAAKK
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHH
AGVKVISYDRLILNTDAVDYYVTFDSVQVGQQQGQYLVDHATGKGNPLYLYAGALSDNNS
CCCEEEEECEEEEECCCEEEEEEECCCHHHHHCCCEEEEECCCCCCEEEEEEEEECCCCC
FLFFQGAWEVLQPKIADGTFVIANSDKANALKDKKDLTRQEMADIIGQITTNWDFNTAKN
EEEECCCHHHHCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LAQANLAKAPKDLKGNVFILAPNDGTARSIADTFAADPDIKSYVITGQDAEKPSIQYIID
HHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEC
GKQSMTVFKDVRILVKDAINVALTYLKGGTPESTKTYNNGVKDVPSKPSPVQVVDKNNVK
CCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCCE
QVLIDSGYYKESDFKWSK
EEEECCCCEECCCCCCCC
>Mature Secondary Structure 
SRKGKIFLVLTALLLVVSAFLGGCSSSKQPASSSNSSGKQLAIGIVLPTKDEPRWIQDE
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCHH
TRFQDALKDTPYAAEILFSQGDPAKEKANVETLLSKGIKVLILCPHDANAAAASAEAAKK
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHH
AGVKVISYDRLILNTDAVDYYVTFDSVQVGQQQGQYLVDHATGKGNPLYLYAGALSDNNS
CCCEEEEECEEEEECCCEEEEEEECCCHHHHHCCCEEEEECCCCCCEEEEEEEEECCCCC
FLFFQGAWEVLQPKIADGTFVIANSDKANALKDKKDLTRQEMADIIGQITTNWDFNTAKN
EEEECCCHHHHCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LAQANLAKAPKDLKGNVFILAPNDGTARSIADTFAADPDIKSYVITGQDAEKPSIQYIID
HHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEC
GKQSMTVFKDVRILVKDAINVALTYLKGGTPESTKTYNNGVKDVPSKPSPVQVVDKNNVK
CCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCCE
QVLIDSGYYKESDFKWSK
EEEECCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253785; 2156804; 11743193; 11743194 [H]