Click here to switch to the map view.

The map label for this gene is NuoH [H]

Identifier: 20806653

GI number: 20806653

Start: 126811

End: 127662

Strand: Direct

Name: NuoH [H]

Synonym: TTE0124

Alternate gene names: 20806653

Gene position: 126811-127662 (Clockwise)

Preceding gene: 20806652

Following gene: 20806654

Centisome position: 4.72

GC content: 38.5

Gene sequence:

>852_bases
ATGATTAAAGAAATACTAATATTCGTCGCAGCTGTAATTTTGGCTCCAATAATCGGTGGTTTTTTAAGTGGTATTGACAG
AAAGTTAACAGCTTTTATACAGGGAAGATATGGGCCTCCCATATGGCAGCCTTTTTACGATATAGTTAAGCTTTTATATA
AACAAAAAATACTTGTGAATAATTTTCAGGTTTTTTCTGCATATATGTACCTTTTGACGGCAATCTTATCTGTAGGACTT
TTTGCGATAAAAGCAGACCTTTTAATGATTTTGTTTGTAATGTCTGTGGGACTGGTGTTTTATATAGCAGGTGCTCTTTC
GACTAAATCGCCGTATAGCCAGGTAGGTGCCCAGAGAGAGCTCATGCAGATGCTGGCCTATGAGCCAATGCTCATATTTT
TTGCAATAGCTTTGTACAATGCAACGGGAAGCTTTAACATAGGACAGATAATGACTCACGGAAGGCTTCTTTTGGACTTG
CCTCTTATGTTTATACTCCTGGCTTTGGTATTGACTATAAAGCTTAAGAAATCTCCTTTTGACTTGTCAGCATCAGAACA
CGCCCATCAGGAGCTGGTAAGAGGCATTTTGACAGATTATTCGGGACCGTATCTCGCTTTAATCCACATTGCTGACTGGT
ACGAGATGGTCCTCATACTCATGATGATAGCGATTTTGTGGTCTGAAAACTTGCTCATAGGGGCGCTAATTGCTCTTTTG
ACTTTCTTTTTAGATATAGTGCTTGACAATATAACTGCAAGGATGACAGTTAAGTGGATGCTGGCTTTCAGCTGGACTAT
AAGCATACTGTTTACTATTGTAAATATAGCTTATATCTACTTTAGGAGATGA

Upstream 100 bases:

>100_bases
ACTACTATCTAACTACTTTTGCCACAGAGAAGACTTTGACAAAGTACTTTAATTACGTCTCTATTGCCCTGATTGCGGTT
ATTCTGGGAGGTGCTATTTT

Downstream 100 bases:

>100_bases
GGAAAATGGGTTTAAAGGAGTTTATAGAAAAATCTTTTAAAAAATCCCCTTGGGTTTTGCACTACAACGCGAGCAGCTGT
AATGGCTGTGACATTGAGAT

Product: NADH:ubiquinone oxidoreductase subunit 1 (chain H)

Products: NAD+; ubiquinol

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MIKEILIFVAAVILAPIIGGFLSGIDRKLTAFIQGRYGPPIWQPFYDIVKLLYKQKILVNNFQVFSAYMYLLTAILSVGL
FAIKADLLMILFVMSVGLVFYIAGALSTKSPYSQVGAQRELMQMLAYEPMLIFFAIALYNATGSFNIGQIMTHGRLLLDL
PLMFILLALVLTIKLKKSPFDLSASEHAHQELVRGILTDYSGPYLALIHIADWYEMVLILMMIAILWSENLLIGALIALL
TFFLDIVLDNITARMTVKWMLAFSWTISILFTIVNIAYIYFRR

Sequences:

>Translated_283_residues
MIKEILIFVAAVILAPIIGGFLSGIDRKLTAFIQGRYGPPIWQPFYDIVKLLYKQKILVNNFQVFSAYMYLLTAILSVGL
FAIKADLLMILFVMSVGLVFYIAGALSTKSPYSQVGAQRELMQMLAYEPMLIFFAIALYNATGSFNIGQIMTHGRLLLDL
PLMFILLALVLTIKLKKSPFDLSASEHAHQELVRGILTDYSGPYLALIHIADWYEMVLILMMIAILWSENLLIGALIALL
TFFLDIVLDNITARMTVKWMLAFSWTISILFTIVNIAYIYFRR
>Mature_283_residues
MIKEILIFVAAVILAPIIGGFLSGIDRKLTAFIQGRYGPPIWQPFYDIVKLLYKQKILVNNFQVFSAYMYLLTAILSVGL
FAIKADLLMILFVMSVGLVFYIAGALSTKSPYSQVGAQRELMQMLAYEPMLIFFAIALYNATGSFNIGQIMTHGRLLLDL
PLMFILLALVLTIKLKKSPFDLSASEHAHQELVRGILTDYSGPYLALIHIADWYEMVLILMMIAILWSENLLIGALIALL
TFFLDIVLDNITARMTVKWMLAFSWTISILFTIVNIAYIYFRR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=279, Percent_Identity=27.5985663082437, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI87082115, Length=207, Percent_Identity=32.8502415458937, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788618, Length=307, Percent_Identity=27.6872964169381, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: 1.6.5.3

Molecular weight: Translated: 32042; Mature: 32042

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEILIFVAAVILAPIIGGFLSGIDRKLTAFIQGRYGPPIWQPFYDIVKLLYKQKILVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
NFQVFSAYMYLLTAILSVGLFAIKADLLMILFVMSVGLVFYIAGALSTKSPYSQVGAQRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LMQMLAYEPMLIFFAIALYNATGSFNIGQIMTHGRLLLDLPLMFILLALVLTIKLKKSPF
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DLSASEHAHQELVRGILTDYSGPYLALIHIADWYEMVLILMMIAILWSENLLIGALIALL
CCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TFFLDIVLDNITARMTVKWMLAFSWTISILFTIVNIAYIYFRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKEILIFVAAVILAPIIGGFLSGIDRKLTAFIQGRYGPPIWQPFYDIVKLLYKQKILVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
NFQVFSAYMYLLTAILSVGLFAIKADLLMILFVMSVGLVFYIAGALSTKSPYSQVGAQRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LMQMLAYEPMLIFFAIALYNATGSFNIGQIMTHGRLLLDLPLMFILLALVLTIKLKKSPF
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DLSASEHAHQELVRGILTDYSGPYLALIHIADWYEMVLILMMIAILWSENLLIGALIALL
CCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TFFLDIVLDNITARMTVKWMLAFSWTISILFTIVNIAYIYFRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]