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The map label for this gene is NuoL [H]

Identifier: 20806652

GI number: 20806652

Start: 124928

End: 126814

Strand: Direct

Name: NuoL [H]

Synonym: TTE0123

Alternate gene names: 20806652

Gene position: 124928-126814 (Clockwise)

Preceding gene: 20806651

Following gene: 20806653

Centisome position: 4.65

GC content: 40.12

Gene sequence:

>1887_bases
ATGCAATCAAATCTGCTTTTGTTGAGCATTTTGTTCCCAATCGCAGCAGGAGTAGTTGTGCTTTTTCTGAGGCAATCTAA
CATTAGAAAAGGTTTTATTGCTGTAGCTCTAGCTGTAATCGTTTTATCATCTTTGGTTTTGCTGGGAACTTTAAAAGTTC
CAGATGTGTTTACTGCATCTGACTTCTACGGTATACTGGATGTGGCTATTAAGATAGCAGATTTTGTGCTGCTAGTCTAT
GTAATTTATATCTCCTTTAAAATTAAAGAATGGAGAGCCACATTTTTTGCAGTATTGCAGCTGATTCCGCTCATCTATTT
CGAGTTTTTTATGCTAAAAGAGCATGAGATGGAGATGTTCTTTGTGGATGAGCTGGCCATCATAATGAATCTGATTATTT
CAATTGTGGGCTCGCTAATAGCTCTTTATGCTTTTAGCTACATGGACCATCACGAAGAACACCTCCACGTATCCCCTACA
AGACAGCCAAGATTTTTCGCTATTATCTTGGTGTTTTTGGGAGCAATGAACGGTTTAGTGCTTTCAAACAATTTAATGTG
GATGTACTTCTTCTGGGAAGTTACAACGCTTTCCTCTTTCCTCCTGATAGGACATGACCAGACAGAGGAAGCTATTAGAA
ATGCTGCAAGGGCTTTGTGGATTAACATGATGGGAGGTTTTGCACTACTCCTTGGAATAATATTTATCTATGCAAATTAC
AGCACCATCTCTCTGGTAGAGGTTTTGAAGATACAAAACGCATCTTATATACTTTTGCCGATGTTTTTCATAGCTTTTGC
AGCATTTACGAAGTCAGCTCAGATGCCTTTCCAGAGCTGGCTTTTAGGGGCGATGGTGGCGCCTACTCCTGTATCTGCAC
TTTTGCACTCCAGCACGATGGTTAAAGCGGGAATATATATCTTACTTAGATTTTCACCCCTTTTCAGAGGCACATTGCTG
AGCGATTTTATAGCGGTGTACGGGGCCTTTACCTTTTTAGCTACTGCCTTTTTAGCTGTGAGCCAGAGCAATGCCAAGAG
AATACTGGCGTACTCTACGGTTTCTAACCTGGGCCTTATGATTGCGAGCATTGGAATAAATACCTCCAGTGCTATTGCTG
CGGCTATACTGCTTCTGATATTCCATGCTATATCCAAGGCACTTCTTTTCTTGAGCGTTGGGACTATTGAACAGCACATT
GGAAGCAGAGACATAGAAGATATGAAGGGCTTAATAAACAAAATGCCTGTAACGACTTCTATAACTTTTGTTGGGATACT
CACGCTGATGGCAGCTCCATTTGGGATGCTTGTGTCAAAGTGGCTCATACTGGAGGTCGCTGCGGGTAGTATAATTATAT
CCATCATACTGGCTATAGGAAGCGCTCTTACAGTGCTTTATTACACAAGGTGGATAGGGAATATCCTATCAGGCACTTAT
TTCACAAAGAAAGAACAGGAACGTATGGATGTTCCAGTTGGGATATCTCTGTATAGTTTAACAGGTTTGGCAGTGATTTT
AAGCCTTATGATTGTGAGCCTTTACAACATGCTGGTGATACCTCAGATTGTAAATATGAAAATGGATGTGGCTTTAAAAG
CTGCGAGAGGTTATTTGTACACGGCTAGCGGAGGATTTATGATTTATCCTATTTTCCTCACAATAGGATTGGCTATAGTT
TTGTCTTTGATATCTATCCAAAAAGCTTCAAAGACGGCTGTAAAAGTTACTCCTTACAATGCAGGGCTTAATTATGTAGA
AGAAAAGAGCTTTGATGTTAAAAACTACTATCTAACTACTTTTGCCACAGAGAAGACTTTGACAAAGTACTTTAATTACG
TCTCTATTGCCCTGATTGCGGTTATTCTGGGAGGTGCTATTTTATGA

Upstream 100 bases:

>100_bases
ATTATTGACTTTGTAGTTTTAAAACTTTATAATCATTGTAGAAGGTTTATCAATTAATTAGAATCCTGTATACAATGTGT
AGAATAGGTGAGGTGTTTGT

Downstream 100 bases:

>100_bases
TTAAAGAAATACTAATATTCGTCGCAGCTGTAATTTTGGCTCCAATAATCGGTGGTTTTTTAAGTGGTATTGACAGAAAG
TTAACAGCTTTTATACAGGG

Product: NADH:ubiquinone oxidoreductase subunit 5 (chain L)/multisubunit Na+/H+ antiporter, MnhA subunit

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MQSNLLLLSILFPIAAGVVVLFLRQSNIRKGFIAVALAVIVLSSLVLLGTLKVPDVFTASDFYGILDVAIKIADFVLLVY
VIYISFKIKEWRATFFAVLQLIPLIYFEFFMLKEHEMEMFFVDELAIIMNLIISIVGSLIALYAFSYMDHHEEHLHVSPT
RQPRFFAIILVFLGAMNGLVLSNNLMWMYFFWEVTTLSSFLLIGHDQTEEAIRNAARALWINMMGGFALLLGIIFIYANY
STISLVEVLKIQNASYILLPMFFIAFAAFTKSAQMPFQSWLLGAMVAPTPVSALLHSSTMVKAGIYILLRFSPLFRGTLL
SDFIAVYGAFTFLATAFLAVSQSNAKRILAYSTVSNLGLMIASIGINTSSAIAAAILLLIFHAISKALLFLSVGTIEQHI
GSRDIEDMKGLINKMPVTTSITFVGILTLMAAPFGMLVSKWLILEVAAGSIIISIILAIGSALTVLYYTRWIGNILSGTY
FTKKEQERMDVPVGISLYSLTGLAVILSLMIVSLYNMLVIPQIVNMKMDVALKAARGYLYTASGGFMIYPIFLTIGLAIV
LSLISIQKASKTAVKVTPYNAGLNYVEEKSFDVKNYYLTTFATEKTLTKYFNYVSIALIAVILGGAIL

Sequences:

>Translated_628_residues
MQSNLLLLSILFPIAAGVVVLFLRQSNIRKGFIAVALAVIVLSSLVLLGTLKVPDVFTASDFYGILDVAIKIADFVLLVY
VIYISFKIKEWRATFFAVLQLIPLIYFEFFMLKEHEMEMFFVDELAIIMNLIISIVGSLIALYAFSYMDHHEEHLHVSPT
RQPRFFAIILVFLGAMNGLVLSNNLMWMYFFWEVTTLSSFLLIGHDQTEEAIRNAARALWINMMGGFALLLGIIFIYANY
STISLVEVLKIQNASYILLPMFFIAFAAFTKSAQMPFQSWLLGAMVAPTPVSALLHSSTMVKAGIYILLRFSPLFRGTLL
SDFIAVYGAFTFLATAFLAVSQSNAKRILAYSTVSNLGLMIASIGINTSSAIAAAILLLIFHAISKALLFLSVGTIEQHI
GSRDIEDMKGLINKMPVTTSITFVGILTLMAAPFGMLVSKWLILEVAAGSIIISIILAIGSALTVLYYTRWIGNILSGTY
FTKKEQERMDVPVGISLYSLTGLAVILSLMIVSLYNMLVIPQIVNMKMDVALKAARGYLYTASGGFMIYPIFLTIGLAIV
LSLISIQKASKTAVKVTPYNAGLNYVEEKSFDVKNYYLTTFATEKTLTKYFNYVSIALIAVILGGAIL
>Mature_628_residues
MQSNLLLLSILFPIAAGVVVLFLRQSNIRKGFIAVALAVIVLSSLVLLGTLKVPDVFTASDFYGILDVAIKIADFVLLVY
VIYISFKIKEWRATFFAVLQLIPLIYFEFFMLKEHEMEMFFVDELAIIMNLIISIVGSLIALYAFSYMDHHEEHLHVSPT
RQPRFFAIILVFLGAMNGLVLSNNLMWMYFFWEVTTLSSFLLIGHDQTEEAIRNAARALWINMMGGFALLLGIIFIYANY
STISLVEVLKIQNASYILLPMFFIAFAAFTKSAQMPFQSWLLGAMVAPTPVSALLHSSTMVKAGIYILLRFSPLFRGTLL
SDFIAVYGAFTFLATAFLAVSQSNAKRILAYSTVSNLGLMIASIGINTSSAIAAAILLLIFHAISKALLFLSVGTIEQHI
GSRDIEDMKGLINKMPVTTSITFVGILTLMAAPFGMLVSKWLILEVAAGSIIISIILAIGSALTVLYYTRWIGNILSGTY
FTKKEQERMDVPVGISLYSLTGLAVILSLMIVSLYNMLVIPQIVNMKMDVALKAARGYLYTASGGFMIYPIFLTIGLAIV
LSLISIQKASKTAVKVTPYNAGLNYVEEKSFDVKNYYLTTFATEKTLTKYFNYVSIALIAVILGGAIL

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=325, Percent_Identity=39.3846153846154, Blast_Score=156, Evalue=7e-38,
Organism=Escherichia coli, GI1788829, Length=381, Percent_Identity=31.496062992126, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788614, Length=417, Percent_Identity=33.0935251798561, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788831, Length=338, Percent_Identity=30.7692307692308, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788827, Length=414, Percent_Identity=27.7777777777778, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788613, Length=401, Percent_Identity=27.6807980049875, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI145693160, Length=410, Percent_Identity=22.1951219512195, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI2367154, Length=206, Percent_Identity=25.2427184466019, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 69392; Mature: 69392

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSNLLLLSILFPIAAGVVVLFLRQSNIRKGFIAVALAVIVLSSLVLLGTLKVPDVFTAS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECH
DFYGILDVAIKIADFVLLVYVIYISFKIKEWRATFFAVLQLIPLIYFEFFMLKEHEMEMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FVDELAIIMNLIISIVGSLIALYAFSYMDHHEEHLHVSPTRQPRFFAIILVFLGAMNGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCEE
LSNNLMWMYFFWEVTTLSSFLLIGHDQTEEAIRNAARALWINMMGGFALLLGIIFIYANY
EECCEEEEHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
STISLVEVLKIQNASYILLPMFFIAFAAFTKSAQMPFQSWLLGAMVAPTPVSALLHSSTM
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
VKAGIYILLRFSPLFRGTLLSDFIAVYGAFTFLATAFLAVSQSNAKRILAYSTVSNLGLM
HHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHH
IASIGINTSSAIAAAILLLIFHAISKALLFLSVGTIEQHIGSRDIEDMKGLINKMPVTTS
HHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
ITFVGILTLMAAPFGMLVSKWLILEVAAGSIIISIILAIGSALTVLYYTRWIGNILSGTY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FTKKEQERMDVPVGISLYSLTGLAVILSLMIVSLYNMLVIPQIVNMKMDVALKAARGYLY
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEE
TASGGFMIYPIFLTIGLAIVLSLISIQKASKTAVKVTPYNAGLNYVEEKSFDVKNYYLTT
EECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEE
FATEKTLTKYFNYVSIALIAVILGGAIL
EHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQSNLLLLSILFPIAAGVVVLFLRQSNIRKGFIAVALAVIVLSSLVLLGTLKVPDVFTAS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECH
DFYGILDVAIKIADFVLLVYVIYISFKIKEWRATFFAVLQLIPLIYFEFFMLKEHEMEMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FVDELAIIMNLIISIVGSLIALYAFSYMDHHEEHLHVSPTRQPRFFAIILVFLGAMNGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCEE
LSNNLMWMYFFWEVTTLSSFLLIGHDQTEEAIRNAARALWINMMGGFALLLGIIFIYANY
EECCEEEEHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
STISLVEVLKIQNASYILLPMFFIAFAAFTKSAQMPFQSWLLGAMVAPTPVSALLHSSTM
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
VKAGIYILLRFSPLFRGTLLSDFIAVYGAFTFLATAFLAVSQSNAKRILAYSTVSNLGLM
HHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHH
IASIGINTSSAIAAAILLLIFHAISKALLFLSVGTIEQHIGSRDIEDMKGLINKMPVTTS
HHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
ITFVGILTLMAAPFGMLVSKWLILEVAAGSIIISIILAIGSALTVLYYTRWIGNILSGTY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FTKKEQERMDVPVGISLYSLTGLAVILSLMIVSLYNMLVIPQIVNMKMDVALKAARGYLY
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEE
TASGGFMIYPIFLTIGLAIVLSLISIQKASKTAVKVTPYNAGLNYVEEKSFDVKNYYLTT
EECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEE
FATEKTLTKYFNYVSIALIAVILGGAIL
EHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]