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The map label for this gene is NuoB [H]

Identifier: 20806654

GI number: 20806654

Start: 127668

End: 128135

Strand: Direct

Name: NuoB [H]

Synonym: TTE0125

Alternate gene names: 20806654

Gene position: 127668-128135 (Clockwise)

Preceding gene: 20806653

Following gene: 20806655

Centisome position: 4.75

GC content: 39.74

Gene sequence:

>468_bases
ATGGGTTTAAAGGAGTTTATAGAAAAATCTTTTAAAAAATCCCCTTGGGTTTTGCACTACAACGCGAGCAGCTGTAATGG
CTGTGACATTGAGATTTTAGCCTGTATGACACCTATATATGATATGGAAAGATTTGGAATGATGAATGTGGGCAATCCTA
AGCATGCGGACATTTTGGTGGTTACAGGCTCTGTAAATACTAGAAATAAGGATGTGCTCAAAAACATATACGACCAGATG
CCAGAGCCTAAAGTGGTAGTAGCAGTTGGAGTTTGTGCGGCAACTGGCGGAGTTTTTAGAGATACTTACAATGTTTTGGG
AGGAGTTGACAAGGTAATCCCTGTTGATGTATATGTTCCTGGCTGTCCTGCTAAACCGGAAGCCATAATAGACGGCCTTT
ACAAGGCCACAGAGATTTTAAAGGAAAAATATAAAAAAATGGGTAAAGCTGAAGAGGTGTTAAAATGA

Upstream 100 bases:

>100_bases
CAAGGATGACAGTTAAGTGGATGCTGGCTTTCAGCTGGACTATAAGCATACTGTTTACTATTGTAAATATAGCTTATATC
TACTTTAGGAGATGAGGAAA

Downstream 100 bases:

>100_bases
TTAAGACGAGGGAAGTTAAGTTGGAAGAGGTTGAAAAAGAGGCAAAGTATTATTACGACAATGGATACAGGTTTGTGACA
GAGACATGTCTTGAAGAAGA

Product: NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductase

Products: NAD+; ubiquinol

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MGLKEFIEKSFKKSPWVLHYNASSCNGCDIEILACMTPIYDMERFGMMNVGNPKHADILVVTGSVNTRNKDVLKNIYDQM
PEPKVVVAVGVCAATGGVFRDTYNVLGGVDKVIPVDVYVPGCPAKPEAIIDGLYKATEILKEKYKKMGKAEEVLK

Sequences:

>Translated_155_residues
MGLKEFIEKSFKKSPWVLHYNASSCNGCDIEILACMTPIYDMERFGMMNVGNPKHADILVVTGSVNTRNKDVLKNIYDQM
PEPKVVVAVGVCAATGGVFRDTYNVLGGVDKVIPVDVYVPGCPAKPEAIIDGLYKATEILKEKYKKMGKAEEVLK
>Mature_154_residues
GLKEFIEKSFKKSPWVLHYNASSCNGCDIEILACMTPIYDMERFGMMNVGNPKHADILVVTGSVNTRNKDVLKNIYDQMP
EPKVVVAVGVCAATGGVFRDTYNVLGGVDKVIPVDVYVPGCPAKPEAIIDGLYKATEILKEKYKKMGKAEEVLK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=143, Percent_Identity=32.8671328671329, Blast_Score=118, Evalue=3e-27,
Organism=Escherichia coli, GI1789074, Length=149, Percent_Identity=40.9395973154362, Blast_Score=139, Evalue=7e-35,
Organism=Escherichia coli, GI1788834, Length=141, Percent_Identity=39.7163120567376, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1788624, Length=147, Percent_Identity=37.4149659863946, Blast_Score=110, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17509685, Length=141, Percent_Identity=38.2978723404255, Blast_Score=116, Evalue=4e-27,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=36.551724137931, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=36.551724137931, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24651058, Length=145, Percent_Identity=36.551724137931, Blast_Score=118, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 17061; Mature: 16930

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKEFIEKSFKKSPWVLHYNASSCNGCDIEILACMTPIYDMERFGMMNVGNPKHADILV
CCHHHHHHHHHCCCCEEEEECCCCCCCCCEEHHHHHCCHHHHHHCCCCCCCCCCCCEEEE
VTGSVNTRNKDVLKNIYDQMPEPKVVVAVGVCAATGGVFRDTYNVLGGVDKVIPVDVYVP
EECCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHCCCCCEEEEEEEEC
GCPAKPEAIIDGLYKATEILKEKYKKMGKAEEVLK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
GLKEFIEKSFKKSPWVLHYNASSCNGCDIEILACMTPIYDMERFGMMNVGNPKHADILV
CHHHHHHHHHCCCCEEEEECCCCCCCCCEEHHHHHCCHHHHHHCCCCCCCCCCCCEEEE
VTGSVNTRNKDVLKNIYDQMPEPKVVVAVGVCAATGGVFRDTYNVLGGVDKVIPVDVYVP
EECCCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHHHHHCCCCCEEEEEEEEC
GCPAKPEAIIDGLYKATEILKEKYKKMGKAEEVLK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]