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The map label for this gene is nusB [H]

Identifier: 203284032

GI number: 203284032

Start: 116428

End: 116868

Strand: Reverse

Name: nusB [H]

Synonym: BDU_110

Alternate gene names: 203284032

Gene position: 116868-116428 (Counterclockwise)

Preceding gene: 203284033

Following gene: 203284031

Centisome position: 12.54

GC content: 27.89

Gene sequence:

>441_bases
TTGGTTATGGATTTGAGACATAAGGCTAGGGTTTTAGCTTTTCAAAAAATTTACAGTATTGATGTTAACTGTAAGGCAAA
GGATAATATTTTTGATATTTTTAGTTTTGAAGAGAATGGGATAGATTTAGATGGAGATTTAAAGTTATTTTATTCTGTTC
TAGTTAATGGTACTTATGATAATTTAGAGTCTATTGACAAATTAATTAGTGGTATTTCACTTAATTGGCGTTTGGATCGT
ATGGATAAGGTTGATCTTGCTATATTAAGAATGAGTGTATATTCACTTAAGTTTCAAGATTTAGATGTTCCTAAAAGGGT
TATAATAGATGAGGCTATTTTGATTGCTAAAAAATATGGCAATAAAAATTCTGATAAGTTTGTCAATGGTATACTTGATG
CTTTGTTAAAAGATATGGAGAGTTCTTTTGAAAACAAATAG

Upstream 100 bases:

>100_bases
TGTTGCTAAAATACAACAAGAAATTTATGATAAACTCAATAAGTCTGCTAGTATACAAATTTTGGATTCTAGTCTAAAAT
GAAGATGATATATTTATATA

Downstream 100 bases:

>100_bases
GTTATTGTTATTTTTTATTTTGTTTTTAATCTTTTTTGTATCAATTTTTGTTTATTTGAGCTTAAATCCATATGTTTTGT
ATATATTAAAGGGAGAAAGG

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MVMDLRHKARVLAFQKIYSIDVNCKAKDNIFDIFSFEENGIDLDGDLKLFYSVLVNGTYDNLESIDKLISGISLNWRLDR
MDKVDLAILRMSVYSLKFQDLDVPKRVIIDEAILIAKKYGNKNSDKFVNGILDALLKDMESSFENK

Sequences:

>Translated_146_residues
MVMDLRHKARVLAFQKIYSIDVNCKAKDNIFDIFSFEENGIDLDGDLKLFYSVLVNGTYDNLESIDKLISGISLNWRLDR
MDKVDLAILRMSVYSLKFQDLDVPKRVIIDEAILIAKKYGNKNSDKFVNGILDALLKDMESSFENK
>Mature_146_residues
MVMDLRHKARVLAFQKIYSIDVNCKAKDNIFDIFSFEENGIDLDGDLKLFYSVLVNGTYDNLESIDKLISGISLNWRLDR
MDKVDLAILRMSVYSLKFQDLDVPKRVIIDEAILIAKKYGNKNSDKFVNGILDALLKDMESSFENK

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=124, Percent_Identity=34.6774193548387, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 16806; Mature: 16806

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMDLRHKARVLAFQKIYSIDVNCKAKDNIFDIFSFEENGIDLDGDLKLFYSVLVNGTYD
CCCCCHHHHHHHHHHHHHEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCC
NLESIDKLISGISLNWRLDRMDKVDLAILRMSVYSLKFQDLDVPKRVIIDEAILIAKKYG
CHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCC
NKNSDKFVNGILDALLKDMESSFENK
CCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVMDLRHKARVLAFQKIYSIDVNCKAKDNIFDIFSFEENGIDLDGDLKLFYSVLVNGTYD
CCCCCHHHHHHHHHHHHHEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCC
NLESIDKLISGISLNWRLDRMDKVDLAILRMSVYSLKFQDLDVPKRVIIDEAILIAKKYG
CHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCC
NKNSDKFVNGILDALLKDMESSFENK
CCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]