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The map label for this gene is HybF

Identifier: 20094983

GI number: 20094983

Start: 1564142

End: 1564570

Strand: Reverse

Name: HybF

Synonym: MK1547

Alternate gene names: 20094983

Gene position: 1564570-1564142 (Counterclockwise)

Preceding gene: 20094986

Following gene: 20094982

Centisome position: 92.31

GC content: 57.58

Gene sequence:

>429_bases
TTGCACGAGCTGAGTGTGGCTCAATCCGTACTCGAAACCGTGTTAGATGTCGCGCGAAAGCGAGGTGCCGAAAGGGTGCT
CTCGGTCCGACTCCGGATAGGTGAATTCACGCTACTGAATCCGGAGCAACTACGGTTCTGTCTGGAAGTGCTCGCAGAAG
GAACGCCCGTAGAGGGGGCGAAGTTCGAGATCGAGATCGAACGTGGGTACTTCAAGTGTGCGGAATGCGGGCACAGATGG
CGCCCGGAGGACGAAAGTCTAAAGGATCCTTCGCTGCATACCGCGTTCGACCTGTCCGAGTTGACCGAACTGCTGGATCT
TAAATGCCCGAAATGTGGTTCCCGAGCGGTGAAGCTGGACGGTGGGGATGCCTGCTCGATCGAGTCCGTACGGCTTGAGG
TGCCGGGTGAGCAGCATGCACAAGGTTGA

Upstream 100 bases:

>100_bases
GTCTCCGGGTCCCGCTCCTACTAGTACGAGCTTACCGGCCACTCCTCATCCCCCGGACCACGGACCTGATCGTGCTTTAA
GTTCCGAGGGTGGGGTCCGC

Downstream 100 bases:

>100_bases
AGTCGACGTCTCCGAAGATCTGCTCGAGGTCAACCGTAACTTAGCCCGCGAGGTTCGGGAAACACTGGACGAGCACAACG
TCCGGGCAGTGGAGGTACTG

Product: zinc finger protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MHELSVAQSVLETVLDVARKRGAERVLSVRLRIGEFTLLNPEQLRFCLEVLAEGTPVEGAKFEIEIERGYFKCAECGHRW
RPEDESLKDPSLHTAFDLSELTELLDLKCPKCGSRAVKLDGGDACSIESVRLEVPGEQHAQG

Sequences:

>Translated_142_residues
MHELSVAQSVLETVLDVARKRGAERVLSVRLRIGEFTLLNPEQLRFCLEVLAEGTPVEGAKFEIEIERGYFKCAECGHRW
RPEDESLKDPSLHTAFDLSELTELLDLKCPKCGSRAVKLDGGDACSIESVRLEVPGEQHAQG
>Mature_142_residues
MHELSVAQSVLETVLDVARKRGAERVLSVRLRIGEFTLLNPEQLRFCLEVLAEGTPVEGAKFEIEIERGYFKCAECGHRW
RPEDESLKDPSLHTAFDLSELTELLDLKCPKCGSRAVKLDGGDACSIESVRLEVPGEQHAQG

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPA_METKA (Q8TV53)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614830.1
- GeneID:   1478142
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1547
- NMPDR:   fig|190192.1.peg.1543
- HOGENOM:   HBG688671
- OMA:   MHELSYA
- ProtClustDB:   CLSK634698
- BioCyc:   MKAN190192:MK1547-MONOMER
- HAMAP:   MF_00213
- InterPro:   IPR000688
- InterPro:   IPR020538
- TIGRFAMs:   TIGR00100

Pfam domain/function: PF01155 HypA

EC number: NA

Molecular weight: Translated: 15806; Mature: 15806

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELSVAQSVLETVLDVARKRGAERVLSVRLRIGEFTLLNPEQLRFCLEVLAEGTPVEGA
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHCCCCCCCC
KFEIEIERGYFKCAECGHRWRPEDESLKDPSLHTAFDLSELTELLDLKCPKCGSRAVKLD
EEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCEEEEEC
GGDACSIESVRLEVPGEQHAQG
CCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MHELSVAQSVLETVLDVARKRGAERVLSVRLRIGEFTLLNPEQLRFCLEVLAEGTPVEGA
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEECHHHHHHHHHHHHCCCCCCCC
KFEIEIERGYFKCAECGHRWRPEDESLKDPSLHTAFDLSELTELLDLKCPKCGSRAVKLD
EEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCEEEEEC
GGDACSIESVRLEVPGEQHAQG
CCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014