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The map label for this gene is HypB_2 [H]

Identifier: 20094982

GI number: 20094982

Start: 1563499

End: 1564155

Strand: Reverse

Name: HypB_2 [H]

Synonym: MK1546

Alternate gene names: 20094982

Gene position: 1564155-1563499 (Counterclockwise)

Preceding gene: 20094983

Following gene: 20094980

Centisome position: 92.28

GC content: 56.47

Gene sequence:

>657_bases
ATGCACAAGGTTGAAGTCGACGTCTCCGAAGATCTGCTCGAGGTCAACCGTAACTTAGCCCGCGAGGTTCGGGAAACACT
GGACGAGCACAACGTCCGGGCAGTGGAGGTACTGGGATCTATCGGTTCCGGAAAGACCTCGCTCATCGAGTGGATCGTGA
AGGAATACGGGGACGAGTACTCCTTCGCGGTCATCGCAGGAGACGTGGTGAGCGAATACGACGAGCGCAGGTTCAAGGAC
CTAGGGGTGCCGACAGTAGGTCTCAACACGGGTCGTGAGTGCCATCTGGACGCTCATATGGTACAACACGGTTTAGAGCA
TCTCGAGGAACTGACGGACCTGAACGAGGTGGACGTGCTGTTCATAGAAAACGTGGGAAACCTCGTATGCCCAGCCGATT
TCCCTATCGGCGCCCACCTACGCGTTATCGTGGTCTCGGCAACGGAGGGTGAGGACGTCATCGGCAAGCATCCGATGATG
ATCCGGAAAGGCGACGTGTTGGTCGTTAATAAGATCGATCTGGCAGACGCCTGCGGTGTCTCTCCGGAGACGATGGTCCG
TACGGCTAAGGAGATAAATCCGGACCTCGAAGTATACCTCACGAGCATCAAGACGGGAGAAGGGATGGCGGAACTCGCCG
AGAGACTGCTCCCTTAA

Upstream 100 bases:

>100_bases
GATCTTAAATGCCCGAAATGTGGTTCCCGAGCGGTGAAGCTGGACGGTGGGGATGCCTGCTCGATCGAGTCCGTACGGCT
TGAGGTGCCGGGTGAGCAGC

Downstream 100 bases:

>100_bases
CCTTCTTCCTCTTCCTCCTTGATGAGGACCGCGTTAACGACGGGTTCCTGACCCGGTCGATTCGTGACCTTCGCGAGCCC
GATCTCCGTCCGAATGATCG

Product: Ni2+-binding GTPase involved in regulation of expression and maturation of hydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MHKVEVDVSEDLLEVNRNLAREVRETLDEHNVRAVEVLGSIGSGKTSLIEWIVKEYGDEYSFAVIAGDVVSEYDERRFKD
LGVPTVGLNTGRECHLDAHMVQHGLEHLEELTDLNEVDVLFIENVGNLVCPADFPIGAHLRVIVVSATEGEDVIGKHPMM
IRKGDVLVVNKIDLADACGVSPETMVRTAKEINPDLEVYLTSIKTGEGMAELAERLLP

Sequences:

>Translated_218_residues
MHKVEVDVSEDLLEVNRNLAREVRETLDEHNVRAVEVLGSIGSGKTSLIEWIVKEYGDEYSFAVIAGDVVSEYDERRFKD
LGVPTVGLNTGRECHLDAHMVQHGLEHLEELTDLNEVDVLFIENVGNLVCPADFPIGAHLRVIVVSATEGEDVIGKHPMM
IRKGDVLVVNKIDLADACGVSPETMVRTAKEINPDLEVYLTSIKTGEGMAELAERLLP
>Mature_218_residues
MHKVEVDVSEDLLEVNRNLAREVRETLDEHNVRAVEVLGSIGSGKTSLIEWIVKEYGDEYSFAVIAGDVVSEYDERRFKD
LGVPTVGLNTGRECHLDAHMVQHGLEHLEELTDLNEVDVLFIENVGNLVCPADFPIGAHLRVIVVSATEGEDVIGKHPMM
IRKGDVLVVNKIDLADACGVSPETMVRTAKEINPDLEVYLTSIKTGEGMAELAERLLP

Specific function: Could be involved in nickel binding and accumulation [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=209, Percent_Identity=38.2775119617225, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 24069; Mature: 24069

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKVEVDVSEDLLEVNRNLAREVRETLDEHNVRAVEVLGSIGSGKTSLIEWIVKEYGDEY
CCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCE
SFAVIAGDVVSEYDERRFKDLGVPTVGLNTGRECHLDAHMVQHGLEHLEELTDLNEVDVL
EEEEEECHHHHHHHHHHHHHCCCCEECCCCCCEECHHHHHHHHHHHHHHHHHCCCCCEEE
FIENVGNLVCPADFPIGAHLRVIVVSATEGEDVIGKHPMMIRKGDVLVVNKIDLADACGV
EEECCCCEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCEEEEEECCCHHHCCC
SPETMVRTAKEINPDLEVYLTSIKTGEGMAELAERLLP
CHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MHKVEVDVSEDLLEVNRNLAREVRETLDEHNVRAVEVLGSIGSGKTSLIEWIVKEYGDEY
CCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCE
SFAVIAGDVVSEYDERRFKDLGVPTVGLNTGRECHLDAHMVQHGLEHLEELTDLNEVDVL
EEEEEECHHHHHHHHHHHHHCCCCEECCCCCCEECHHHHHHHHHHHHHHHHHCCCCCEEE
FIENVGNLVCPADFPIGAHLRVIVVSATEGEDVIGKHPMMIRKGDVLVVNKIDLADACGV
EEECCCCEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCEEEEEECCCHHHCCC
SPETMVRTAKEINPDLEVYLTSIKTGEGMAELAERLLP
CHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]