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The map label for this gene is AnsB

Identifier: 20094945

GI number: 20094945

Start: 1524636

End: 1526012

Strand: Reverse

Name: AnsB

Synonym: MK1509

Alternate gene names: 20094945

Gene position: 1526012-1524636 (Counterclockwise)

Preceding gene: 20094952

Following gene: 20094942

Centisome position: 90.03

GC content: 60.35

Gene sequence:

>1377_bases
GTGAGGGTGTTGTCCGAGTACAAAGGTAAGGCCGGGGAGTTACTACGGGAGCACGGGCTGAGTGTCGGCGACCGGGTTCG
TATAGTCCGGGACGATGGGGTGGTCGTGGAAGGCATCATCATGCCCAGGTCGGAACTTGGAGACGATGAGCACATCGTCG
TCAAAATGGACAACGGTTACAATGTAGGGGTACGTGTCGACAGGATCGAGAAGCTCGAAGCCCCGGGTGAAGGACATGAA
CCTAGCTTCAAGCCGATGGAGGGAGAGATCGAGTACGATCCTAAGCTTCCCAACGTATCGGTGATGTCGACGGGCGGTAC
CATCGCATGCCGGGTGGACTACGAGACCGGGGCCGTGAAACCTGCGTTCACAGCCGAGGAGCTCGTCGGAGCGGTCCCGG
AGCTGCTCGACGTGATCAACATCGTAGACGCCAGGGCCGTGCTCGATCTGCTCAGCGAGAATATGGAGCCCAAACACTGG
ATGAAGATCGCCGAAGAGGTCGTGGACGCTCTCTCGGACCCGGATGTAGAGGGTGTCGTGATCGGACACGGAACGGACAC
GATGGCGTTCACGGCCGCGGCGCTATCGTTCGTCATCGAGGGGCTTAACGGGCCGGTCGTGTTGGTCGGAGCGCAGCGGA
GTTCGGACAGGCCGTCTTCGGACGCGGCCTCGAACCTCATCGCGGCGTGCGCCTTCGCGGGCGACGGAGAGGTGGGTGAG
GTCACCGTCTGCATGCACGGGTGGACCTCCGATGAGGTATGCCTCGTGCATCGGGGAGTGAGGGTCAGGAAGATGCACAC
GTCACGCAGGGACGCGTTCAGGTCGGTGGAGTCCATCCCTATCGCGAAGGTGGACGTGAAGGATCTCAGGAACCCTAAAA
TCGAGTTCCTCCGGAGCGATTACCGGAGGCCGGAGGACGGTGAGCCTGAGATCTCAGGCGGGTTCGAGGAAAAAGTGGCC
TTGGTGAAGTTCGCACCCGGTATGGATCCCGAGGTCTTAGACTTCTACGTGGATCGGGGTTACCGGGGGATCGTCCTCGA
AGGCACCGGTTTGGGCCACGTCTCGGAGCAGTGGTTGGAGTCGATCGAGCGGGCGGTGGACGACGGGATCGCCGTCGTGA
TGACATCTCAGTGCTTGTACGGTCGTGTGAACATGAACGTGTACAGGACCGGACGACTGCTGAGGGCTGTGGGTGTGATA
CCGGGTGAGGATATGCTGCCAGAGGTGGCTTACGTGAAGCTCATGTACGTGCTCGATAGAACCGACGACATCAAGGAGGT
CGAGAGGCTCATGAGGACGAACATCGCTGGAGAGATCGAAGGTGGTCGAGTTCTGGGAGGATTCGAACCCGCGGACGGTC
CACATCATCGCCTGTAG

Upstream 100 bases:

>100_bases
CAAGTGTTTAACCGCGCGGTACACGCTGTTCAAGTTGCCGACGCCGTACTCTACTATCGCCACTTTCAAGCCGAGTCCCC
CTTGGAAGGATGGGTCTGGG

Downstream 100 bases:

>100_bases
CCGGAGTCGCCGAGGCGTGACTTGGCGGTGTTCAGTTCGGTACAGGCGTCGCTTCAGAACACCTCGATTTCGCCCTCCAC
GATACGTTCCGTGAGCTCGG

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRVLSEYKGKAGELLREHGLSVGDRVRIVRDDGVVVEGIIMPRSELGDDEHIVVKMDNGYNVGVRVDRIEKLEAPGEGHE
PSFKPMEGEIEYDPKLPNVSVMSTGGTIACRVDYETGAVKPAFTAEELVGAVPELLDVINIVDARAVLDLLSENMEPKHW
MKIAEEVVDALSDPDVEGVVIGHGTDTMAFTAAALSFVIEGLNGPVVLVGAQRSSDRPSSDAASNLIAACAFAGDGEVGE
VTVCMHGWTSDEVCLVHRGVRVRKMHTSRRDAFRSVESIPIAKVDVKDLRNPKIEFLRSDYRRPEDGEPEISGGFEEKVA
LVKFAPGMDPEVLDFYVDRGYRGIVLEGTGLGHVSEQWLESIERAVDDGIAVVMTSQCLYGRVNMNVYRTGRLLRAVGVI
PGEDMLPEVAYVKLMYVLDRTDDIKEVERLMRTNIAGEIEGGRVLGGFEPADGPHHRL

Sequences:

>Translated_458_residues
MRVLSEYKGKAGELLREHGLSVGDRVRIVRDDGVVVEGIIMPRSELGDDEHIVVKMDNGYNVGVRVDRIEKLEAPGEGHE
PSFKPMEGEIEYDPKLPNVSVMSTGGTIACRVDYETGAVKPAFTAEELVGAVPELLDVINIVDARAVLDLLSENMEPKHW
MKIAEEVVDALSDPDVEGVVIGHGTDTMAFTAAALSFVIEGLNGPVVLVGAQRSSDRPSSDAASNLIAACAFAGDGEVGE
VTVCMHGWTSDEVCLVHRGVRVRKMHTSRRDAFRSVESIPIAKVDVKDLRNPKIEFLRSDYRRPEDGEPEISGGFEEKVA
LVKFAPGMDPEVLDFYVDRGYRGIVLEGTGLGHVSEQWLESIERAVDDGIAVVMTSQCLYGRVNMNVYRTGRLLRAVGVI
PGEDMLPEVAYVKLMYVLDRTDDIKEVERLMRTNIAGEIEGGRVLGGFEPADGPHHRL
>Mature_458_residues
MRVLSEYKGKAGELLREHGLSVGDRVRIVRDDGVVVEGIIMPRSELGDDEHIVVKMDNGYNVGVRVDRIEKLEAPGEGHE
PSFKPMEGEIEYDPKLPNVSVMSTGGTIACRVDYETGAVKPAFTAEELVGAVPELLDVINIVDARAVLDLLSENMEPKHW
MKIAEEVVDALSDPDVEGVVIGHGTDTMAFTAAALSFVIEGLNGPVVLVGAQRSSDRPSSDAASNLIAACAFAGDGEVGE
VTVCMHGWTSDEVCLVHRGVRVRKMHTSRRDAFRSVESIPIAKVDVKDLRNPKIEFLRSDYRRPEDGEPEISGGFEEKVA
LVKFAPGMDPEVLDFYVDRGYRGIVLEGTGLGHVSEQWLESIERAVDDGIAVVMTSQCLYGRVNMNVYRTGRLLRAVGVI
PGEDMLPEVAYVKLMYVLDRTDDIKEVERLMRTNIAGEIEGGRVLGGFEPADGPHHRL

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=307, Percent_Identity=28.6644951140065, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788065, Length=350, Percent_Identity=30.5714285714286, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789327, Length=348, Percent_Identity=30.4597701149425, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI193207372, Length=303, Percent_Identity=32.3432343234323, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI193207374, Length=303, Percent_Identity=32.3432343234323, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17558278, Length=303, Percent_Identity=32.3432343234323, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17558280, Length=303, Percent_Identity=32.3432343234323, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323189, Length=274, Percent_Identity=28.1021897810219, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323187, Length=274, Percent_Identity=28.1021897810219, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323186, Length=274, Percent_Identity=28.1021897810219, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323184, Length=274, Percent_Identity=28.1021897810219, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320527, Length=349, Percent_Identity=22.9226361031519, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24645530, Length=290, Percent_Identity=33.448275862069, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24639609, Length=325, Percent_Identity=29.2307692307692, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_METKA (Q8TV84)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614792.1
- ProteinModelPortal:   Q8TV84
- GeneID:   1478104
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1509
- NMPDR:   fig|190192.1.peg.1505
- HOGENOM:   HBG497495
- OMA:   KMKSGYN
- ProtClustDB:   PRK04183
- BioCyc:   MKAN190192:MK1509-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 50120; Mature: 50120

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 107-107 ACT_SITE 185-185 ACT_SITE 186-186 ACT_SITE 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLSEYKGKAGELLREHGLSVGDRVRIVRDDGVVVEGIIMPRSELGDDEHIVVKMDNGY
CCCHHHHCCHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECHHHCCCCCEEEEEECCCC
NVGVRVDRIEKLEAPGEGHEPSFKPMEGEIEYDPKLPNVSVMSTGGTIACRVDYETGAVK
EEEEEEEHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCEEEEEEECCCCCCC
PAFTAEELVGAVPELLDVINIVDARAVLDLLSENMEPKHWMKIAEEVVDALSDPDVEGVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEE
IGHGTDTMAFTAAALSFVIEGLNGPVVLVGAQRSSDRPSSDAASNLIAACAFAGDGEVGE
EECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEECCCCCCCE
VTVCMHGWTSDEVCLVHRGVRVRKMHTSRRDAFRSVESIPIAKVDVKDLRNPKIEFLRSD
EEEEEECCCCCCEEEEECCCEEEEHHHHHHHHHHHHHCCCCEEECHHHCCCCHHHHHHHH
YRRPEDGEPEISGGFEEKVALVKFAPGMDPEVLDFYVDRGYRGIVLEGTGLGHVSEQWLE
CCCCCCCCCCCCCCHHHCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
SIERAVDDGIAVVMTSQCLYGRVNMNVYRTGRLLRAVGVIPGEDMLPEVAYVKLMYVLDR
HHHHHHHCCEEEEEECCHHEEEECCEEEHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHCC
TDDIKEVERLMRTNIAGEIEGGRVLGGFEPADGPHHRL
CCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRVLSEYKGKAGELLREHGLSVGDRVRIVRDDGVVVEGIIMPRSELGDDEHIVVKMDNGY
CCCHHHHCCHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECHHHCCCCCEEEEEECCCC
NVGVRVDRIEKLEAPGEGHEPSFKPMEGEIEYDPKLPNVSVMSTGGTIACRVDYETGAVK
EEEEEEEHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCEEEEEEECCCCCCC
PAFTAEELVGAVPELLDVINIVDARAVLDLLSENMEPKHWMKIAEEVVDALSDPDVEGVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEE
IGHGTDTMAFTAAALSFVIEGLNGPVVLVGAQRSSDRPSSDAASNLIAACAFAGDGEVGE
EECCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEECCCCCCCE
VTVCMHGWTSDEVCLVHRGVRVRKMHTSRRDAFRSVESIPIAKVDVKDLRNPKIEFLRSD
EEEEEECCCCCCEEEEECCCEEEEHHHHHHHHHHHHHCCCCEEECHHHCCCCHHHHHHHH
YRRPEDGEPEISGGFEEKVALVKFAPGMDPEVLDFYVDRGYRGIVLEGTGLGHVSEQWLE
CCCCCCCCCCCCCCHHHCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
SIERAVDDGIAVVMTSQCLYGRVNMNVYRTGRLLRAVGVIPGEDMLPEVAYVKLMYVLDR
HHHHHHHCCEEEEEECCHHEEEECCEEEHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHCC
TDDIKEVERLMRTNIAGEIEGGRVLGGFEPADGPHHRL
CCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014