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The map label for this gene is CbiB [H]

Identifier: 20094952

GI number: 20094952

Start: 1529214

End: 1530110

Strand: Reverse

Name: CbiB [H]

Synonym: MK1516

Alternate gene names: 20094952

Gene position: 1530110-1529214 (Counterclockwise)

Preceding gene: 20094953

Following gene: 20094945

Centisome position: 90.27

GC content: 63.66

Gene sequence:

>897_bases
ATGCCGTTGGATCCCCTTCTCGCCGCGTATCTGGCGGTGGCTCTGGACCTGGTCCTGGGGGATCCGCCGAACCGTTTCCA
CCCGGTGGCGTGGTGCGGCCGGGTGATGGAGCTCGTGGAGAAGAGGGTACGGCGCGGGCGTGTCCTCGACGTGGTCTGGG
GCGGATTAACCCTGGCGGTCGGTTGCGGGTTCCTGCTGGGAGTGTGTTACCCGCTCCACTACATCCCGATGGTTGGAGAT
GCTCTGGTCCTGTGGGTGTGTATTTCCGTACGGGGACTCGTCGAGCACTTGCTCCCGGTAGAGCGGGAGCTCCGGGAGGG
GAACTTAGACGGGGCTCGACAGGCCGTGAGGTGGCTCGTCAGTCGGGACGTAAGCCGTCTAAACGGAGCGGAAGTAGCTT
CGGCTGCGGTGGAAAGTTGCTTCGAGAACCTACTGGACTCCGTCGTGGGCCCCTTGTGGTGGTACTATCTCCTCGGGTGG
CCGGGTGCGGTCATGTACCGAGCGACGAACGTCGCGGACGCCATGTTCGGGTACCGGGGCGAGTACGAGATGTTCGGTAA
GATTCCGGCGAGGTTGGACGACTTGCTGAACGTGCTGTCACTGCCCGTATGCGCCGCGGCACTCACGGTAACCCTACCTT
GGTGGATACTCCGGGCGAGACTCCGGTCACTGTTGCGGGCTGCACGGGACGTCCCGAGCCCCAGTTCCTGGTTGCCCATG
TACGTGGGGGCCTGCGCGCTGGGAGTCCGACTGGAGAAACCGAACGTGTACGTCCTAAAAGGAGGGGATCGACTCCCGAC
ACCCGACGACGTGCGTCGCGCGGCTCAGATCGCCGTGATATTCGGGCTGGTCGCCCCGGGTATCACGTACGTCGCAGCTG
GTCTTTTAAGTCCCTAA

Upstream 100 bases:

>100_bases
TGAACCCCGCCGAACTAGCCGCGGTGCGTGCCGGGTGGAAGGAGTACCGCCTCGATGAGATTCCGACCCCCGAGGACGTC
CTCAACGCCCTACGGCGGTG

Downstream 100 bases:

>100_bases
TCACCTTTAGTACGCGCTCGCCACGCTCGGTAAGCCTGTAAAACGTTCTACCGCCTTCGGAGCGCTTAGTGACCAAACCG
ACCTCCAGGAGCTTCTTCAA

Product: cobalamin biosynthesis protein CobD/CbiB

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPLDPLLAAYLAVALDLVLGDPPNRFHPVAWCGRVMELVEKRVRRGRVLDVVWGGLTLAVGCGFLLGVCYPLHYIPMVGD
ALVLWVCISVRGLVEHLLPVERELREGNLDGARQAVRWLVSRDVSRLNGAEVASAAVESCFENLLDSVVGPLWWYYLLGW
PGAVMYRATNVADAMFGYRGEYEMFGKIPARLDDLLNVLSLPVCAAALTVTLPWWILRARLRSLLRAARDVPSPSSWLPM
YVGACALGVRLEKPNVYVLKGGDRLPTPDDVRRAAQIAVIFGLVAPGITYVAAGLLSP

Sequences:

>Translated_298_residues
MPLDPLLAAYLAVALDLVLGDPPNRFHPVAWCGRVMELVEKRVRRGRVLDVVWGGLTLAVGCGFLLGVCYPLHYIPMVGD
ALVLWVCISVRGLVEHLLPVERELREGNLDGARQAVRWLVSRDVSRLNGAEVASAAVESCFENLLDSVVGPLWWYYLLGW
PGAVMYRATNVADAMFGYRGEYEMFGKIPARLDDLLNVLSLPVCAAALTVTLPWWILRARLRSLLRAARDVPSPSSWLPM
YVGACALGVRLEKPNVYVLKGGDRLPTPDDVRRAAQIAVIFGLVAPGITYVAAGLLSP
>Mature_297_residues
PLDPLLAAYLAVALDLVLGDPPNRFHPVAWCGRVMELVEKRVRRGRVLDVVWGGLTLAVGCGFLLGVCYPLHYIPMVGDA
LVLWVCISVRGLVEHLLPVERELREGNLDGARQAVRWLVSRDVSRLNGAEVASAAVESCFENLLDSVVGPLWWYYLLGWP
GAVMYRATNVADAMFGYRGEYEMFGKIPARLDDLLNVLSLPVCAAALTVTLPWWILRARLRSLLRAARDVPSPSSWLPMY
VGACALGVRLEKPNVYVLKGGDRLPTPDDVRRAAQIAVIFGLVAPGITYVAAGLLSP

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 32685; Mature: 32554

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLDPLLAAYLAVALDLVLGDPPNRFHPVAWCGRVMELVEKRVRRGRVLDVVWGGLTLAV
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GCGFLLGVCYPLHYIPMVGDALVLWVCISVRGLVEHLLPVERELREGNLDGARQAVRWLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SRDVSRLNGAEVASAAVESCFENLLDSVVGPLWWYYLLGWPGAVMYRATNVADAMFGYRG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
EYEMFGKIPARLDDLLNVLSLPVCAAALTVTLPWWILRARLRSLLRAARDVPSPSSWLPM
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
YVGACALGVRLEKPNVYVLKGGDRLPTPDDVRRAAQIAVIFGLVAPGITYVAAGLLSP
HHHHHHHCCEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]