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The map label for this gene is RIO1_2 [H]

Identifier: 20094835

GI number: 20094835

Start: 1423066

End: 1423941

Strand: Reverse

Name: RIO1_2 [H]

Synonym: MK1399

Alternate gene names: 20094835

Gene position: 1423941-1423066 (Counterclockwise)

Preceding gene: 20094836

Following gene: 20094834

Centisome position: 84.01

GC content: 61.99

Gene sequence:

>876_bases
TTGGAGCAACTGGGACGTGCACTGTTAGAAACGGTGCAGAAAGACGATCCTGAGCCGTTCCTGAGGGCGCTGGAGGAGAC
GGAGGTAGATCCGGAAGACGCGGAGCGGTTCCTGGAAGCGTTGCGGATACAGCGGGAGAAAGGGAGGATCTCTGACGAAA
CCCTGGAGGCCGTCGAAGATGCGCTCTTCAAGGTGTCAGAGTCCGAGGAACGTGAGATCCCGGAGCCTGACCCTCTGGCG
GAGTACGCCGGAGTTGATCGCATTCTCGGGACGATCATGACCGGAAAGGAGGCGGATGTCCTGTTGGCGGAACGTGACGG
GGAGAAGGTAGCCCTGAAGGTGTATCGCGCGCACACCGGGTACGAGGAACGGCACGAGGAGCGGGTTTACAGGTTAGAGG
ACGGTGAGGTACGACGGATCGAGCGCGGGGATGCGGCCCTGAGGGAGTTCTCCAGGCTGCGACGCGCGTACGAGGCGGGT
GTGAGGGTACCGAAGCCCTACGATGCCCGTCCGGGCCTCATCGTGATGGAGTACATTCCGGGCGAACCCCTCTACCGAGC
GCCGGATCTGGACGACCCGGGATCGGTGTTAGAGGATCTGCTGGACCAGGTCGTGCGGTTGGCCGTGGATGCCGAGCTGG
TTCACGGCGATCTGAGCGCGTTCAACGTCCTCGTGGGCGACGACGGTGTACCGTATATCATCGACCTGTCCGAAGCTGTG
AAAGTGAAGGAGCCGGGGGCGTTTGAGACACTCCGGCGGGACGTGAAGAACCTCGTATCGTTCTTCGAGCGGAAGTACGG
GGTGTCGGCGGACGTAGACGAGGTCGTAGAGAGGGTGAGGCGAGGTGTTTACGGGACCGATCGTGGCCGTAGGTGA

Upstream 100 bases:

>100_bases
AGACTGTCGGGGAGCTCGAGGGCCTCGACCTGACGGAGTAAGGCCCCGTTCCCTCGTGGCTCCGGTGTCGGGATTCCAGG
GACCACCGGGGGCCTCGGAG

Downstream 100 bases:

>100_bases
ATATGTGGAGGGACTGGGTGAAGGCCGTCGCTACGTCAGTATTCCTTATTACCGGAGGGAAATCGAACGTGTGATCGGGG
CGCGACCGTTCCCGGGGACG

Product: serine/threonine protein kinase

Products: NA

Alternate protein names: AfRio1 [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MEQLGRALLETVQKDDPEPFLRALEETEVDPEDAERFLEALRIQREKGRISDETLEAVEDALFKVSESEEREIPEPDPLA
EYAGVDRILGTIMTGKEADVLLAERDGEKVALKVYRAHTGYEERHEERVYRLEDGEVRRIERGDAALREFSRLRRAYEAG
VRVPKPYDARPGLIVMEYIPGEPLYRAPDLDDPGSVLEDLLDQVVRLAVDAELVHGDLSAFNVLVGDDGVPYIIDLSEAV
KVKEPGAFETLRRDVKNLVSFFERKYGVSADVDEVVERVRRGVYGTDRGRR

Sequences:

>Translated_291_residues
MEQLGRALLETVQKDDPEPFLRALEETEVDPEDAERFLEALRIQREKGRISDETLEAVEDALFKVSESEEREIPEPDPLA
EYAGVDRILGTIMTGKEADVLLAERDGEKVALKVYRAHTGYEERHEERVYRLEDGEVRRIERGDAALREFSRLRRAYEAG
VRVPKPYDARPGLIVMEYIPGEPLYRAPDLDDPGSVLEDLLDQVVRLAVDAELVHGDLSAFNVLVGDDGVPYIIDLSEAV
KVKEPGAFETLRRDVKNLVSFFERKYGVSADVDEVVERVRRGVYGTDRGRR
>Mature_291_residues
MEQLGRALLETVQKDDPEPFLRALEETEVDPEDAERFLEALRIQREKGRISDETLEAVEDALFKVSESEEREIPEPDPLA
EYAGVDRILGTIMTGKEADVLLAERDGEKVALKVYRAHTGYEERHEERVYRLEDGEVRRIERGDAALREFSRLRRAYEAG
VRVPKPYDARPGLIVMEYIPGEPLYRAPDLDDPGSVLEDLLDQVVRLAVDAELVHGDLSAFNVLVGDDGVPYIIDLSEAV
KVKEPGAFETLRRDVKNLVSFFERKYGVSADVDEVVERVRRGVYGTDRGRR

Specific function: Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis [H]

COG id: COG1718

COG function: function code TD; Serine/threonine protein kinase involved in cell cycle control

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI229331967, Length=200, Percent_Identity=29, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI229331971, Length=200, Percent_Identity=29, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI23510356, Length=210, Percent_Identity=33.8095238095238, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI23510358, Length=142, Percent_Identity=36.6197183098592, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115532073, Length=194, Percent_Identity=35.0515463917526, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17556977, Length=194, Percent_Identity=31.4432989690722, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI32564178, Length=185, Percent_Identity=28.6486486486486, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324693, Length=228, Percent_Identity=31.140350877193, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324122, Length=190, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24649986, Length=189, Percent_Identity=29.6296296296296, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24662811, Length=211, Percent_Identity=30.3317535545024, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356879, Length=211, Percent_Identity=30.3317535545024, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920700, Length=217, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR018934
- InterPro:   IPR000687
- InterPro:   IPR018935 [H]

Pfam domain/function: PF01163 RIO1 [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 33054; Mature: 33054

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00109 PROTEIN_KINASE_TYR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQLGRALLETVQKDDPEPFLRALEETEVDPEDAERFLEALRIQREKGRISDETLEAVED
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ALFKVSESEEREIPEPDPLAEYAGVDRILGTIMTGKEADVLLAERDGEKVALKVYRAHTG
HHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHCC
YEERHEERVYRLEDGEVRRIERGDAALREFSRLRRAYEAGVRVPKPYDARPGLIVMEYIP
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
GEPLYRAPDLDDPGSVLEDLLDQVVRLAVDAELVHGDLSAFNVLVGDDGVPYIIDLSEAV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCEEEECHHHH
KVKEPGAFETLRRDVKNLVSFFERKYGVSADVDEVVERVRRGVYGTDRGRR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEQLGRALLETVQKDDPEPFLRALEETEVDPEDAERFLEALRIQREKGRISDETLEAVED
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ALFKVSESEEREIPEPDPLAEYAGVDRILGTIMTGKEADVLLAERDGEKVALKVYRAHTG
HHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHCC
YEERHEERVYRLEDGEVRRIERGDAALREFSRLRRAYEAGVRVPKPYDARPGLIVMEYIP
HHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
GEPLYRAPDLDDPGSVLEDLLDQVVRLAVDAELVHGDLSAFNVLVGDDGVPYIIDLSEAV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCEEEECHHHH
KVKEPGAFETLRRDVKNLVSFFERKYGVSADVDEVVERVRRGVYGTDRGRR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]