Click here to switch to the map view.

The map label for this gene is PksG [C]

Identifier: 20094815

GI number: 20094815

Start: 1405824

End: 1406876

Strand: Reverse

Name: PksG [C]

Synonym: MK1379

Alternate gene names: 20094815

Gene position: 1406876-1405824 (Counterclockwise)

Preceding gene: 20094816

Following gene: 20094814

Centisome position: 83.0

GC content: 62.77

Gene sequence:

>1053_bases
TTGATACCTTCGGAGCGTGTCGGGATCGTCGGGTACGGTGCTTACGTTCCACGGTACCGGATTAAGGCGGAAGAGATCGC
GGCCGTTTGGGGAGATGACGTCGATTCCATCAAGTCGGGGCTGATGATCGAGGAGAAGAGCGTCCCATCGGAGACCGAAG
ATTCCGCCACTATCGCCGTTGAAGCCGCGAAGAACGCCGTGGCACGGGCTGAGATAGATCCCAAGGACATAGGTGCGATC
TACGTGGGATCTGAGTCACCACCTTACGCCGTCAAACCCACCGCCACGATCGTAGCGGCCGCCATCGGAGCGACGCCCGA
TCTCACGGCGGCCGATTACGAGTTCGCGTGCAAGGCCGGAACAGCTGCCATCCAAACGTGCGCGGGTCTCGTGGCTTCGG
GTATGATCAAGTACGGGCTCGCCATCGGCGCGGACACGGCTCAAGGGGCTCCGGGAGACCCGCTGGAGTACACGGCGGCG
GCCGGTGGAGCCGCGTTCGTGATCGGGAGGAAGAAGCTTGTCGCCGAGATGGAAGGCACCTACTCGTACACCACGGACAC
TCCGGACTTCTGGCGCCGAGAAGGGCAACCTTATCCGCGTCACGGCGGCAGGTTTACCGGTGCCCCGGCGTACTTCAAGC
ACATCATCAGGGCTGCCCGTGGACTGATGGAGGAACTCGACTTGAGCCCCGAGGACTTCGACTACGCGGTGTTCCATCAG
CCCAACGGCAAGTTCCCGAGGAAGGTCGCGCGCAGCCTGGGGTTCGAGCCCGAGCAGGTCGAGCCGACCATCGTCGTCGA
CCGAGTAGGTAACACGTACTCGGGGTCGTCGCTGCTCGGGTTCACGGCGGCACTGGACCGCGCCGAGCCAGGTGACCGCA
TCCTGGTCGTCTCGTACGGTTCGGGTGCGGGTAGCGACGCGTTCAGCTTCGTCGTGACGGAGCGTATCGAGGAGGTCCGG
GAGAAGGCCCCGCTGCTGGAGGAGTACCTGGAGGATCGCGTGTACGTGACGTATGGAGAGTACGCGAAGATGAAGAAGAA
GCTCAAGTTCTGA

Upstream 100 bases:

>100_bases
CGGAGGTAGAGGGTATCAATCTGGCCACGACTACCGAGTCCATTTCGAGGATCTCGAAAAGGCTTAAGGAGCTTACCGAA
GTTCAGCCGGGGGATCGTGG

Downstream 100 bases:

>100_bases
GGGGATGGTGTGCGTGCGTTTCCGTCGGGACGTCGCCATCGTCGGCGTGGGTATGACGAGGTTCGGAGAGCTGTGGGAGC
GGTCCTTCGACGACCTGTTC

Product: hypothetical protein

Products: 3-oxoacyl-[acyl-carrier protein]; CO2; [acyl-carrier protein]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MIPSERVGIVGYGAYVPRYRIKAEEIAAVWGDDVDSIKSGLMIEEKSVPSETEDSATIAVEAAKNAVARAEIDPKDIGAI
YVGSESPPYAVKPTATIVAAAIGATPDLTAADYEFACKAGTAAIQTCAGLVASGMIKYGLAIGADTAQGAPGDPLEYTAA
AGGAAFVIGRKKLVAEMEGTYSYTTDTPDFWRREGQPYPRHGGRFTGAPAYFKHIIRAARGLMEELDLSPEDFDYAVFHQ
PNGKFPRKVARSLGFEPEQVEPTIVVDRVGNTYSGSSLLGFTAALDRAEPGDRILVVSYGSGAGSDAFSFVVTERIEEVR
EKAPLLEEYLEDRVYVTYGEYAKMKKKLKF

Sequences:

>Translated_350_residues
MIPSERVGIVGYGAYVPRYRIKAEEIAAVWGDDVDSIKSGLMIEEKSVPSETEDSATIAVEAAKNAVARAEIDPKDIGAI
YVGSESPPYAVKPTATIVAAAIGATPDLTAADYEFACKAGTAAIQTCAGLVASGMIKYGLAIGADTAQGAPGDPLEYTAA
AGGAAFVIGRKKLVAEMEGTYSYTTDTPDFWRREGQPYPRHGGRFTGAPAYFKHIIRAARGLMEELDLSPEDFDYAVFHQ
PNGKFPRKVARSLGFEPEQVEPTIVVDRVGNTYSGSSLLGFTAALDRAEPGDRILVVSYGSGAGSDAFSFVVTERIEEVR
EKAPLLEEYLEDRVYVTYGEYAKMKKKLKF
>Mature_350_residues
MIPSERVGIVGYGAYVPRYRIKAEEIAAVWGDDVDSIKSGLMIEEKSVPSETEDSATIAVEAAKNAVARAEIDPKDIGAI
YVGSESPPYAVKPTATIVAAAIGATPDLTAADYEFACKAGTAAIQTCAGLVASGMIKYGLAIGADTAQGAPGDPLEYTAA
AGGAAFVIGRKKLVAEMEGTYSYTTDTPDFWRREGQPYPRHGGRFTGAPAYFKHIIRAARGLMEELDLSPEDFDYAVFHQ
PNGKFPRKVARSLGFEPEQVEPTIVVDRVGNTYSGSSLLGFTAALDRAEPGDRILVVSYGSGAGSDAFSFVVTERIEEVR
EKAPLLEEYLEDRVYVTYGEYAKMKKKLKF

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Catalyzes The First Condensation Reaction Which Initiates Fatty Acid Synthesis And May Therefore Play A Role In Governing The

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0219 family

Homologues:

Organism=Escherichia coli, GI1787333, Length=311, Percent_Identity=25.7234726688103, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1379_METKA (Q8TVL0)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614662.1
- ProteinModelPortal:   Q8TVL0
- SMR:   Q8TVL0
- GeneID:   1477974
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1379
- NMPDR:   fig|190192.1.peg.1375
- HOGENOM:   HBG297330
- OMA:   ESKPYAV
- ProtClustDB:   PRK04262
- BioCyc:   MKAN190192:MK1379-MONOMER
- HAMAP:   MF_01409
- InterPro:   IPR013747
- InterPro:   IPR004656
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038
- Gene3D:   G3DSA:3.40.47.10
- TIGRFAMs:   TIGR00748

Pfam domain/function: PF08541 ACP_syn_III_C; PF01154 HMG_CoA_synt_N; SSF53901 Thiolase-like

EC number: 2.3.1.41

Molecular weight: Translated: 37599; Mature: 37599

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPSERVGIVGYGAYVPRYRIKAEEIAAVWGDDVDSIKSGLMIEEKSVPSETEDSATIAV
CCCCCCEEEEEECCCCCCEEECHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCEEEEE
EAAKNAVARAEIDPKDIGAIYVGSESPPYAVKPTATIVAAAIGATPDLTAADYEFACKAG
EHHHCHHHHCCCCHHHCCEEEECCCCCCEEECCHHHHHHHHHCCCCCCCCCCCHHHHCCC
TAAIQTCAGLVASGMIKYGLAIGADTAQGAPGDPLEYTAAAGGAAFVIGRKKLVAEMEGT
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEECCCCEEEEEEHHHHHHHHCCC
YSYTTDTPDFWRREGQPYPRHGGRFTGAPAYFKHIIRAARGLMEELDLSPEDFDYAVFHQ
EEECCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PNGKFPRKVARSLGFEPEQVEPTIVVDRVGNTYSGSSLLGFTAALDRAEPGDRILVVSYG
CCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEC
SGAGSDAFSFVVTERIEEVREKAPLLEEYLEDRVYVTYGEYAKMKKKLKF
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCEEEEECHHHHHHHHCCC
>Mature Secondary Structure
MIPSERVGIVGYGAYVPRYRIKAEEIAAVWGDDVDSIKSGLMIEEKSVPSETEDSATIAV
CCCCCCEEEEEECCCCCCEEECHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCEEEEE
EAAKNAVARAEIDPKDIGAIYVGSESPPYAVKPTATIVAAAIGATPDLTAADYEFACKAG
EHHHCHHHHCCCCHHHCCEEEECCCCCCEEECCHHHHHHHHHCCCCCCCCCCCHHHHCCC
TAAIQTCAGLVASGMIKYGLAIGADTAQGAPGDPLEYTAAAGGAAFVIGRKKLVAEMEGT
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEECCCCEEEEEEHHHHHHHHCCC
YSYTTDTPDFWRREGQPYPRHGGRFTGAPAYFKHIIRAARGLMEELDLSPEDFDYAVFHQ
EEECCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
PNGKFPRKVARSLGFEPEQVEPTIVVDRVGNTYSGSSLLGFTAALDRAEPGDRILVVSYG
CCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEC
SGAGSDAFSFVVTERIEEVREKAPLLEEYLEDRVYVTYGEYAKMKKKLKF
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCEEEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acyl-[acyl-carrier protein]; malonyl-[acyl-carrier protein]

Specific reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014