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The map label for this gene is CCA1

Identifier: 20094802

GI number: 20094802

Start: 1393825

End: 1395282

Strand: Reverse

Name: CCA1

Synonym: MK1366

Alternate gene names: 20094802

Gene position: 1395282-1393825 (Counterclockwise)

Preceding gene: 20094803

Following gene: 20094801

Centisome position: 82.32

GC content: 66.46

Gene sequence:

>1458_bases
GTGAGCCTCGAAGAGGTCCTCGACGAGATCAGACGAGAGGTCACGCCGGACCCCGAGGAGCGCGAGCTGGTGGAAGGCTT
CGCACGTAGGATCCTGAGCGAGGTTAGGGACAGGCTGAAGGAGCGCGACCCCGACGCCGAGGTCGAGCTCATCGGCTCGG
TAGCCAGGGACACCTGGCTGCCGGGCGCCTCGGACGTCGACGTGTTCTGCGTGTTCCCGAAGGACCGCGACCTGGACGAA
ATCGTGGAGGTCACGCTGGAGGTAGGACGCGAAGCCATCGAGGCGCTCGGCGGGGAGGCCCGGGAGGAGTACGCGCATCA
CCCTTACATCGGGGGCGAGGTCGAGCACCGGGGTCGGACCTTCGAGGTCGACGTCGTACCCTGCTACGATACCGAACCGG
GCGAAGTTATCACCCCGGTCGATCGCACGCCGCACCACAACCGCTACGTCGAGGAGCACCTGGAGGACACTGTCGAGGCG
AGGCTGCTCAAAGCTTTCGTCAAGGCGATCGACGCGTACGGTGCGGAGGTCCGCGTGAAGGGGTTCTCCGGCTACCTCTG
CGAGCTTCTCGCGATCCACTACGGGTCCTTCGAGGAGGTACTCCGGGAGGCCGTGAGGACCTGGAGGCCCGGCTTCGTCA
TCGACCTCGAGGGGTTCGTGGGTGAAGTTTACGAAGACTACGACGAGGTAAGGGAGACGTTCGAGGATCAGGACCCAGCG
CTGATCGTCCTAGACCCCGTGGATCCCGAGCGCAACGTGGCGGCGGCCCTCTCAAGGCGCCAGCTCACCCGCTTCATCCT
GGCGGCCAGGGCGTTCCTAGGGGATCCGTCGCCCGAGTTCTTCCGGGGGAGGAAGCCCGAACCCGTGAGTGGAGAGAAGG
TCAGGGATTGGTTCGAGAGGAACCCCACCCACGTCGTCGCGATCGAGGTTCGGCTGCCGGATGAGGTGGAGGACATCTAC
TGGCCGCAGCTGGAGAAGACGGCTAGGAGCCTCAGCAGGGTGCTGGAGAACGAGGGGTTCGAGGTGCGTCGGTGGCACGT
CATGCGAGACTCCGAGGAGGAGCATGGGTACGTCCTGTTGGAGTTCGAGCACGGAAAACTGCCCGAGCTCGAGTGGCGCG
TGGGCCCGTCGGGTTGGGTCCGGGAGGACAGGGTCCGCGGGTTCGTCCGCGCCCACGGGAGGTTCTGGGTGGAGGAGGAC
GGAAAGCTGGCGACTCGGGCCGAGCGCAAGTTCGTACGTCCTGAGGACCTCCTGGGGCGTCTGGAGGGCGCCGACCGCCA
GACGCTCCTGTCCCACGGTTTCGGGAAGGACCTAGCGCGGTCTTCCGAGGGTGAGGTGAGGCTGCTCTCCGCCGAGGAGC
TGGCCGAGCTGGCCGATCGGGACCCGGAGCTGGGTAAGGCCCTGGCCGAGTTCATGCGTGGCGACCCGCTCTCCGAGCTC
GTGCGGGACCGCCTGTGA

Upstream 100 bases:

>100_bases
GGGATCGGGACTTAACCCTCATAAGCCCGGCACTGGACGATCGGTGCCCACGTTCGCTCCTTCACGATAATCGCTCCAGT
AGCCCGGCGGGGCGGGTTCG

Downstream 100 bases:

>100_bases
CCCGACCCCAATTTTGACGGCTGACGTTTCCAAACCGTGGCATTTATCATGCTATCCCTGCGGTCGTGATAAGGGGGTAG
CACTTGGCCACCGTGGCCGT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSLEEVLDEIRREVTPDPEERELVEGFARRILSEVRDRLKERDPDAEVELIGSVARDTWLPGASDVDVFCVFPKDRDLDE
IVEVTLEVGREAIEALGGEAREEYAHHPYIGGEVEHRGRTFEVDVVPCYDTEPGEVITPVDRTPHHNRYVEEHLEDTVEA
RLLKAFVKAIDAYGAEVRVKGFSGYLCELLAIHYGSFEEVLREAVRTWRPGFVIDLEGFVGEVYEDYDEVRETFEDQDPA
LIVLDPVDPERNVAAALSRRQLTRFILAARAFLGDPSPEFFRGRKPEPVSGEKVRDWFERNPTHVVAIEVRLPDEVEDIY
WPQLEKTARSLSRVLENEGFEVRRWHVMRDSEEEHGYVLLEFEHGKLPELEWRVGPSGWVREDRVRGFVRAHGRFWVEED
GKLATRAERKFVRPEDLLGRLEGADRQTLLSHGFGKDLARSSEGEVRLLSAEELAELADRDPELGKALAEFMRGDPLSEL
VRDRL

Sequences:

>Translated_485_residues
MSLEEVLDEIRREVTPDPEERELVEGFARRILSEVRDRLKERDPDAEVELIGSVARDTWLPGASDVDVFCVFPKDRDLDE
IVEVTLEVGREAIEALGGEAREEYAHHPYIGGEVEHRGRTFEVDVVPCYDTEPGEVITPVDRTPHHNRYVEEHLEDTVEA
RLLKAFVKAIDAYGAEVRVKGFSGYLCELLAIHYGSFEEVLREAVRTWRPGFVIDLEGFVGEVYEDYDEVRETFEDQDPA
LIVLDPVDPERNVAAALSRRQLTRFILAARAFLGDPSPEFFRGRKPEPVSGEKVRDWFERNPTHVVAIEVRLPDEVEDIY
WPQLEKTARSLSRVLENEGFEVRRWHVMRDSEEEHGYVLLEFEHGKLPELEWRVGPSGWVREDRVRGFVRAHGRFWVEED
GKLATRAERKFVRPEDLLGRLEGADRQTLLSHGFGKDLARSSEGEVRLLSAEELAELADRDPELGKALAEFMRGDPLSEL
VRDRL
>Mature_484_residues
SLEEVLDEIRREVTPDPEERELVEGFARRILSEVRDRLKERDPDAEVELIGSVARDTWLPGASDVDVFCVFPKDRDLDEI
VEVTLEVGREAIEALGGEAREEYAHHPYIGGEVEHRGRTFEVDVVPCYDTEPGEVITPVDRTPHHNRYVEEHLEDTVEAR
LLKAFVKAIDAYGAEVRVKGFSGYLCELLAIHYGSFEEVLREAVRTWRPGFVIDLEGFVGEVYEDYDEVRETFEDQDPAL
IVLDPVDPERNVAAALSRRQLTRFILAARAFLGDPSPEFFRGRKPEPVSGEKVRDWFERNPTHVVAIEVRLPDEVEDIYW
PQLEKTARSLSRVLENEGFEVRRWHVMRDSEEEHGYVLLEFEHGKLPELEWRVGPSGWVREDRVRGFVRAHGRFWVEEDG
KLATRAERKFVRPEDLLGRLEGADRQTLLSHGFGKDLARSSEGEVRLLSAEELAELADRDPELGKALAEFMRGDPLSELV
RDRL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG1746

COG function: function code J; tRNA nucleotidyltransferase (CCA-adding enzyme)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_METKA (Q8TGY5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614649.1
- ProteinModelPortal:   Q8TGY5
- GeneID:   1477961
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1366
- NMPDR:   fig|190192.1.peg.1362
- HOGENOM:   HBG645243
- OMA:   CEKIISA
- ProtClustDB:   PRK13300
- BioCyc:   MKAN190192:MK1366-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01264
- InterPro:   IPR008229
- InterPro:   IPR002934
- InterPro:   IPR011068
- InterPro:   IPR015329
- PIRSF:   PIRSF005335
- TIGRFAMs:   TIGR03671

Pfam domain/function: PF01909 NTP_transf_2; PF09249 tRNA_NucTransf2; SSF55003 PAP_C

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 55775; Mature: 55644

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: BINDING 53-53 BINDING 56-56 BINDING 146-146 BINDING 164-164 BINDING 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEEVLDEIRREVTPDPEERELVEGFARRILSEVRDRLKERDPDAEVELIGSVARDTWL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
PGASDVDVFCVFPKDRDLDEIVEVTLEVGREAIEALGGEAREEYAHHPYIGGEVEHRGRT
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEECCCCCE
FEVDVVPCYDTEPGEVITPVDRTPHHNRYVEEHLEDTVEARLLKAFVKAIDAYGAEVRVK
EEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GFSGYLCELLAIHYGSFEEVLREAVRTWRPGFVIDLEGFVGEVYEDYDEVRETFEDQDPA
CCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCE
LIVLDPVDPERNVAAALSRRQLTRFILAARAFLGDPSPEFFRGRKPEPVSGEKVRDWFER
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHC
NPTHVVAIEVRLPDEVEDIYWPQLEKTARSLSRVLENEGFEVRRWHVMRDSEEEHGYVLL
CCCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEE
EFEHGKLPELEWRVGPSGWVREDRVRGFVRAHGRFWVEEDGKLATRAERKFVRPEDLLGR
EECCCCCCCCEEEECCCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCHHHHHHH
LEGADRQTLLSHGFGKDLARSSEGEVRLLSAEELAELADRDPELGKALAEFMRGDPLSEL
HCCCCHHHHHHCCCCHHHHCCCCCCEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
VRDRL
HHHCC
>Mature Secondary Structure 
SLEEVLDEIRREVTPDPEERELVEGFARRILSEVRDRLKERDPDAEVELIGSVARDTWL
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
PGASDVDVFCVFPKDRDLDEIVEVTLEVGREAIEALGGEAREEYAHHPYIGGEVEHRGRT
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEECCCCCE
FEVDVVPCYDTEPGEVITPVDRTPHHNRYVEEHLEDTVEARLLKAFVKAIDAYGAEVRVK
EEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GFSGYLCELLAIHYGSFEEVLREAVRTWRPGFVIDLEGFVGEVYEDYDEVRETFEDQDPA
CCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCE
LIVLDPVDPERNVAAALSRRQLTRFILAARAFLGDPSPEFFRGRKPEPVSGEKVRDWFER
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHC
NPTHVVAIEVRLPDEVEDIYWPQLEKTARSLSRVLENEGFEVRRWHVMRDSEEEHGYVLL
CCCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEE
EFEHGKLPELEWRVGPSGWVREDRVRGFVRAHGRFWVEEDGKLATRAERKFVRPEDLLGR
EECCCCCCCCEEEECCCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCCHHHHHHH
LEGADRQTLLSHGFGKDLARSSEGEVRLLSAEELAELADRDPELGKALAEFMRGDPLSEL
HCCCCHHHHHHCCCCHHHHCCCCCCEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCHHHH
VRDRL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014