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The map label for this gene is frvX [C]

Identifier: 20093821

GI number: 20093821

Start: 368062

End: 369129

Strand: Reverse

Name: frvX [C]

Synonym: MK0383

Alternate gene names: 20093821

Gene position: 369129-368062 (Counterclockwise)

Preceding gene: 20093822

Following gene: 20093820

Centisome position: 21.78

GC content: 60.96

Gene sequence:

>1068_bases
ATGGGAACCGTCGAAACCCTCACGGAACACCTACGCGAGCTGGTCGGCCGTGTGGCTCCACCGGGCTGGGAGGACGAGGT
CCGCGAGTACGTGGAGGCCACACTGGAGAAGTACTGTGATGATGTCCACGTAGATACCTTAGGGAACGTGATCGGTACTA
TCGAAGGCTCCGAGTACGAGGTGATGGTAGCCGCCCACATGGACGAGGTGGGTTTCATCGTCAAGTCCATCGACAAGAAC
GGGTTCATACGGTTCGCGAAGCTCGGCGGTATCGATGACAGGATCTTACCGGGCTCACGCGTGATCATCGTGAACTCCGA
AGGCGAAAAGGTGCCGGGGGTCGTCGGGACCAAGCCTCCGCACATCCAGGAGCCGAAGGATCGGCGGAAAGTGCCCAAGC
ACAAGGACTTGTTCATAGATATCGGCGCCTCAGACAGGGAGGAGGCTGAGGAACTCGTGAGCGTAGGGGATGTCGGGGTC
CTAGCCGGAGAGTTCGTAGAACTGGTAGGATCGAGGGTCAACGGCCGAGGTCTCGACGACAAGATCGGTGTCGCCGTTCT
TCTCGCGCTGGCCGAGCGTCTCGCCGACCTCGACGGAGACCACCCCACGTTTTACCTGGTGGGAACAGTCCAGGAGGAGG
TGGGTCTGAAGGGGGCCAAAACGTCCGCCTTCGAGGTCTACCCGGACGGTGCTGTAGTTCTAGACACTGCCGTAGCCGGT
GACGTGCCGGGAGTGAAGGAGGCGGAGCTGAAGCTCGGGAAGGGGCCGGCGATCACGGTAGTCGACGCGAGTGGTCGGGG
TCTGATCACTCACCCCAAGGTGAGAAAGCTACTGATAGACACCGCGGAGGAGCTGGACATCCCGTATCAGCTCGAGGTAG
GTGAGGGAGGCACCACCGACGCGACGGCCATTCACCTAACCAGAGGTGGCGTTCCGACAGGTGTAGTGGGGATACCGACC
AGGTACCTACACTCGCCGGCGGAGGTGCTGGATCTCGAGGATGCGAAACACGCCCTCGAGCTCGTAGTCGAGGTCGTGCA
AAGGTTCCCGGATTACGTGCCGAGGTGA

Upstream 100 bases:

>100_bases
GGCGGGACTGCAGGACGAGTTCTTCGAGAAGCTTAACGAGATCACGAAAGGAGATTTCGAGAGCAAAATACTGGAGAGGG
AGAGTGTGGAGGGTCCCTGA

Downstream 100 bases:

>100_bases
TGTAAGCGTGCCCGAGTTCGAGCTGTACGTCGAGGACCGTCAGGTCGTCACTCCCGGTGAGTTGTTGGCTAGAGGGCAGG
TGATCGCGAGCGAGGGGACG

Product: hydrolase related to cellulase M

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MGTVETLTEHLRELVGRVAPPGWEDEVREYVEATLEKYCDDVHVDTLGNVIGTIEGSEYEVMVAAHMDEVGFIVKSIDKN
GFIRFAKLGGIDDRILPGSRVIIVNSEGEKVPGVVGTKPPHIQEPKDRRKVPKHKDLFIDIGASDREEAEELVSVGDVGV
LAGEFVELVGSRVNGRGLDDKIGVAVLLALAERLADLDGDHPTFYLVGTVQEEVGLKGAKTSAFEVYPDGAVVLDTAVAG
DVPGVKEAELKLGKGPAITVVDASGRGLITHPKVRKLLIDTAEELDIPYQLEVGEGGTTDATAIHLTRGGVPTGVVGIPT
RYLHSPAEVLDLEDAKHALELVVEVVQRFPDYVPR

Sequences:

>Translated_355_residues
MGTVETLTEHLRELVGRVAPPGWEDEVREYVEATLEKYCDDVHVDTLGNVIGTIEGSEYEVMVAAHMDEVGFIVKSIDKN
GFIRFAKLGGIDDRILPGSRVIIVNSEGEKVPGVVGTKPPHIQEPKDRRKVPKHKDLFIDIGASDREEAEELVSVGDVGV
LAGEFVELVGSRVNGRGLDDKIGVAVLLALAERLADLDGDHPTFYLVGTVQEEVGLKGAKTSAFEVYPDGAVVLDTAVAG
DVPGVKEAELKLGKGPAITVVDASGRGLITHPKVRKLLIDTAEELDIPYQLEVGEGGTTDATAIHLTRGGVPTGVVGIPT
RYLHSPAEVLDLEDAKHALELVVEVVQRFPDYVPR
>Mature_354_residues
GTVETLTEHLRELVGRVAPPGWEDEVREYVEATLEKYCDDVHVDTLGNVIGTIEGSEYEVMVAAHMDEVGFIVKSIDKNG
FIRFAKLGGIDDRILPGSRVIIVNSEGEKVPGVVGTKPPHIQEPKDRRKVPKHKDLFIDIGASDREEAEELVSVGDVGVL
AGEFVELVGSRVNGRGLDDKIGVAVLLALAERLADLDGDHPTFYLVGTVQEEVGLKGAKTSAFEVYPDGAVVLDTAVAGD
VPGVKEAELKLGKGPAITVVDASGRGLITHPKVRKLLIDTAEELDIPYQLEVGEGGTTDATAIHLTRGGVPTGVVGIPTR
YLHSPAEVLDLEDAKHALELVVEVVQRFPDYVPR

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=337, Percent_Identity=37.0919881305638, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI87082417, Length=349, Percent_Identity=33.2378223495702, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1788727, Length=330, Percent_Identity=32.4242424242424, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 38123; Mature: 37992

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTVETLTEHLRELVGRVAPPGWEDEVREYVEATLEKYCDDVHVDTLGNVIGTIEGSEYE
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCEE
VMVAAHMDEVGFIVKSIDKNGFIRFAKLGGIDDRILPGSRVIIVNSEGEKVPGVVGTKPP
EEEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
HIQEPKDRRKVPKHKDLFIDIGASDREEAEELVSVGDVGVLAGEFVELVGSRVNGRGLDD
CCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCH
KIGVAVLLALAERLADLDGDHPTFYLVGTVQEEVGLKGAKTSAFEVYPDGAVVLDTAVAG
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHCCCCCCCCEEEECCCCCEEEEEHHCC
DVPGVKEAELKLGKGPAITVVDASGRGLITHPKVRKLLIDTAEELDIPYQLEVGEGGTTD
CCCCCCHHHEEECCCCEEEEEECCCCCCEECHHHHHHHHHHHHHCCCCEEEEECCCCCCC
ATAIHLTRGGVPTGVVGIPTRYLHSPAEVLDLEDAKHALELVVEVVQRFPDYVPR
CEEEEEECCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GTVETLTEHLRELVGRVAPPGWEDEVREYVEATLEKYCDDVHVDTLGNVIGTIEGSEYE
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCEE
VMVAAHMDEVGFIVKSIDKNGFIRFAKLGGIDDRILPGSRVIIVNSEGEKVPGVVGTKPP
EEEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
HIQEPKDRRKVPKHKDLFIDIGASDREEAEELVSVGDVGVLAGEFVELVGSRVNGRGLDD
CCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCH
KIGVAVLLALAERLADLDGDHPTFYLVGTVQEEVGLKGAKTSAFEVYPDGAVVLDTAVAG
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHCCCCCCCCEEEECCCCCEEEEEHHCC
DVPGVKEAELKLGKGPAITVVDASGRGLITHPKVRKLLIDTAEELDIPYQLEVGEGGTTD
CCCCCCHHHEEECCCCEEEEEECCCCCCEECHHHHHHHHHHHHHCCCCEEEEECCCCCCC
ATAIHLTRGGVPTGVVGIPTRYLHSPAEVLDLEDAKHALELVVEVVQRFPDYVPR
CEEEEEECCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]