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The map label for this gene is HisA

Identifier: 20093802

GI number: 20093802

Start: 352038

End: 352766

Strand: Direct

Name: HisA

Synonym: MK0364

Alternate gene names: 20093802

Gene position: 352038-352766 (Clockwise)

Preceding gene: 20093801

Following gene: 20093804

Centisome position: 20.77

GC content: 59.53

Gene sequence:

>729_bases
TTGGCTTTCGTCGTCATCCCCTCGGTCGATGTAGTCGAGGGGAAGTGTGTGCAGCTTGTCGAAGGAGATCCGGAACGGAG
GACGTTCGAGTCCGATGACCCCGTGGAAACCGCGCACCAGTGGTCCGAGTTTTTCCCCTGGATTCACGTGGTGGACGTCG
ACGCGGCACGCGGTGAGGGGGATAACTCGGATATCATCGGACGGATCTGCGAGGAGGTCGACGCCAAGGTGCAGGTGGGT
GGCGGGATCCGTTCCGCCGAACGCGCGGAAGAGCTCATCGAGCTGGGTGCCGATCGTCTGATAGTGGGAACCGTGGCGTT
CACCGATAAGGATGATTTCTCGAAGATCGTCGACGTGTGTCACGATCACGGTATCGAGGTATTCGTGGCACTGGACGTCA
ACGAGAACCATGAGGTGCTAGTCAGCGGGTGGAAGGAGGACGCGGGAGTCACCCTGGAAGACGCCATAGAGCGCTTCAAC
GAAGTTGCCGACGGGTATCTGACCACCGCGGTGCACGTCGAGGGTAAGGAAATGGGTATCGACGAAAAAGTCGTTGAGAA
GTCGACCGGTGCCACGGATCTCCCCGTGTTGTACGCCGGTGGAATAGCTTCGATCAAGGACGTGAAGCGGGCCAAGGAGG
CCGGAGCGTACGGGGTAGTGATCGGGACCGCCCTGTACCACGGGGACATCGATCCCGTGGCACTACTTGATCTCATGGAG
GAGGATTAG

Upstream 100 bases:

>100_bases
GGACCGGATCACCAGCGAGGTACCTGAGGTAACCAGAGTTTTGTTCGACATAACCCCTAAACCCCCTGCAACGATCGAGT
TCGAGTGAGGGGGACGCGCG

Downstream 100 bases:

>100_bases
GGTATCATCCTCTCGATGGGGAGGACGATGATACCTTCTGCACCCAACTCTTTGGCCCTGAGTACGACCTCCGACACGTC
CTCCTCGTTCACTACCGCGT

Product: phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MAFVVIPSVDVVEGKCVQLVEGDPERRTFESDDPVETAHQWSEFFPWIHVVDVDAARGEGDNSDIIGRICEEVDAKVQVG
GGIRSAERAEELIELGADRLIVGTVAFTDKDDFSKIVDVCHDHGIEVFVALDVNENHEVLVSGWKEDAGVTLEDAIERFN
EVADGYLTTAVHVEGKEMGIDEKVVEKSTGATDLPVLYAGGIASIKDVKRAKEAGAYGVVIGTALYHGDIDPVALLDLME
ED

Sequences:

>Translated_242_residues
MAFVVIPSVDVVEGKCVQLVEGDPERRTFESDDPVETAHQWSEFFPWIHVVDVDAARGEGDNSDIIGRICEEVDAKVQVG
GGIRSAERAEELIELGADRLIVGTVAFTDKDDFSKIVDVCHDHGIEVFVALDVNENHEVLVSGWKEDAGVTLEDAIERFN
EVADGYLTTAVHVEGKEMGIDEKVVEKSTGATDLPVLYAGGIASIKDVKRAKEAGAYGVVIGTALYHGDIDPVALLDLME
ED
>Mature_241_residues
AFVVIPSVDVVEGKCVQLVEGDPERRTFESDDPVETAHQWSEFFPWIHVVDVDAARGEGDNSDIIGRICEEVDAKVQVGG
GIRSAERAEELIELGADRLIVGTVAFTDKDDFSKIVDVCHDHGIEVFVALDVNENHEVLVSGWKEDAGVTLEDAIERFNE
VADGYLTTAVHVEGKEMGIDEKVVEKSTGATDLPVLYAGGIASIKDVKRAKEAGAYGVVIGTALYHGDIDPVALLDLMEE
D

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=234, Percent_Identity=29.9145299145299, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_METKA (Q8TYD6)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613649.1
- ProteinModelPortal:   Q8TYD6
- SMR:   Q8TYD6
- GeneID:   1477667
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0364
- NMPDR:   fig|190192.1.peg.362
- HOGENOM:   HBG541613
- OMA:   GRCQLGG
- BioCyc:   MKAN190192:MK0364-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26223; Mature: 26092

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFVVIPSVDVVEGKCVQLVEGDPERRTFESDDPVETAHQWSEFFPWIHVVDVDAARGEG
CEEEEECCCEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCC
DNSDIIGRICEEVDAKVQVGGGIRSAERAEELIELGADRLIVGTVAFTDKDDFSKIVDVC
CCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHH
HDHGIEVFVALDVNENHEVLVSGWKEDAGVTLEDAIERFNEVADGYLTTAVHVEGKEMGI
HCCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCC
DEKVVEKSTGATDLPVLYAGGIASIKDVKRAKEAGAYGVVIGTALYHGDIDPVALLDLME
CHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEHEEECCCCCHHHHHHHHC
ED
CC
>Mature Secondary Structure 
AFVVIPSVDVVEGKCVQLVEGDPERRTFESDDPVETAHQWSEFFPWIHVVDVDAARGEG
EEEEECCCEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCC
DNSDIIGRICEEVDAKVQVGGGIRSAERAEELIELGADRLIVGTVAFTDKDDFSKIVDVC
CCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHH
HDHGIEVFVALDVNENHEVLVSGWKEDAGVTLEDAIERFNEVADGYLTTAVHVEGKEMGI
HCCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCC
DEKVVEKSTGATDLPVLYAGGIASIKDVKRAKEAGAYGVVIGTALYHGDIDPVALLDLME
CHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEHEEECCCCCHHHHHHHHC
ED
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014