Click here to switch to the map view.

The map label for this gene is glpK [H]

Identifier: 19746596

GI number: 19746596

Start: 1411485

End: 1413011

Strand: Reverse

Name: glpK [H]

Synonym: spyM18_1696

Alternate gene names: 19746596

Gene position: 1413011-1411485 (Counterclockwise)

Preceding gene: 19746597

Following gene: 19746595

Centisome position: 74.56

GC content: 41.72

Gene sequence:

>1527_bases
ATGTCGCAAGAAAAGTATATCATGGCTATTGATCAGGGAACAACAAGTTCACGAGCGATTATTTTCAATCAAAAAGGGGA
AAAGGTTAGCAGTAGCCAAAAAGAATTCCCTCAGATTTTCCCACACGCTGGTTGGGTAGAGCACAATGCTAATCAGATTT
GGAATTCGGTTCAGTCTGTTATTGCGGGTGCTTTTATTGAGTCCAGCATCAAACCAAGCCAAATTGAAGCAATCGGAATT
ACCAACCAACGTGAGACCACAGTGGTTTGGGACAAAAAAACGGGTGTACCTATTTATAATGCTATTGTGTGGCAATCACG
ACAGACGGCACCAATTGCTGAGCAACTCAAACAAGATGGCCATACAAAAATGATCCATGAAAAGACAGGCCTTGTTATTG
ATGCCTACTTTTCTGCAACCAAGATCCGTTGGATTTTAGATCATGTTCCTGGTGCTCAAGAACGAGCTGAAAAAGGCGAG
TTACTTTTTGGAACCATTGATACTTGGCTGGTTTGGAAACTAACAGATGGTGCTGTTCATGTGACGGATTATTCCAATGC
GGCTCGTACCATGCTCTATAATATTAAAGACTTAACATGGGATGATGAGATTTTAGAGCTTTTAAATATTCCAAAAGATA
TGCTTCCAGAGGTTAAATCCAATTCTGAAATTTATGGCAAAACAGCTGCCTTTCATTTTTATGGGGGCGAAGTGCCTATT
TCAGGTATGGCGGGTGACCAACAGGCAGCTTTGTTTGGCCAACTAGCTTTTGAGCCTGGTATGGTGAAAAACACTTATGG
CACCGGTTCTTTCATTATCATGAATACTGGTGATGAGATGCAGTTATCGAGTAATAATCTTTTGACCACCATTGGGTATG
GTATTGGTGGTAAGGTACATTATGCCTTGGAAGGTTCCATCTTTATTGCAGGCTCTGCCATTCAATGGTTGAGAGATGGT
CTTAAAATGATTGAGACTTCACCAGAATCTGAACAGTTCGCTCTAGCATCAACTAGTGACGATGAAGTTTATGTTGTGCC
TGCTTTTACCGGTTTAGGAGCTCCTTACTGGGATTCTAATGCGCGTGGATCTGTCTTTGGCTTGACACGTGGTACCAGCA
AAGAAGACTTTGTCAAAGCAACCTTACAATCTATCGCCTATCAAGTGCGAGATGTTATTGATACCATGCAGGTGGATTCA
GGTATTGATATTCAACAGCTTCGTGTGGATGGCGGTGCAGCCATGAATAATATGCTCATGCAATTTCAGGCTGATATTTT
AGGAATTGATATTGCCCGTGCTAAAAATTTAGAAACAACAGCTCTAGGAGCAGCTTTCTTAGCAGGGCTTGCTGTGGGGT
ATTGGGAAGATATGGATGCTTTGAAAGAGTTAAATGCCACAGGCCAGCTCTTTAAGGCTAGCATGAATGAATCGCGCAAA
GAAAAATTATACAAGGGTTGGAAGCGCGCTGTTAAAGCAACTCAAGTTTTCACTCAAGAAGAAGATGCAGATGACGATGC
AAAATAA

Upstream 100 bases:

>100_bases
AGCTTTTTTTTAGCTTTAAAAATTCTTGATAATATATTTCTGATAAAATTAGCTATAATAGAATCAAATAGGAAAACGCT
TACTTAAAGGAGGGTAATGA

Downstream 100 bases:

>100_bases
CTAGAGGAGGATGGAATGGAATTTTCAAGAGAAACAAGACGCTTAGCTCTCCAAAAAATGCAAGAAAGAGATTTAGACCT
ACTGATTATTGGGGGAGGTA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAGWVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGI
TNQRETTVVWDKKTGVPIYNAIVWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEVKSNSEIYGKTAAFHFYGGEVPI
SGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGDEMQLSSNNLLTTIGYGIGGKVHYALEGSIFIAGSAIQWLRDG
LKMIETSPESEQFALASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDVIDTMQVDS
GIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGLAVGYWEDMDALKELNATGQLFKASMNESRK
EKLYKGWKRAVKATQVFTQEEDADDDAK

Sequences:

>Translated_508_residues
MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAGWVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGI
TNQRETTVVWDKKTGVPIYNAIVWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEVKSNSEIYGKTAAFHFYGGEVPI
SGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGDEMQLSSNNLLTTIGYGIGGKVHYALEGSIFIAGSAIQWLRDG
LKMIETSPESEQFALASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDVIDTMQVDS
GIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGLAVGYWEDMDALKELNATGQLFKASMNESRK
EKLYKGWKRAVKATQVFTQEEDADDDAK
>Mature_507_residues
SQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAGWVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGIT
NQRETTVVWDKKTGVPIYNAIVWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGEL
LFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEVKSNSEIYGKTAAFHFYGGEVPIS
GMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGDEMQLSSNNLLTTIGYGIGGKVHYALEGSIFIAGSAIQWLRDGL
KMIETSPESEQFALASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDVIDTMQVDSG
IDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGLAVGYWEDMDALKELNATGQLFKASMNESRKE
KLYKGWKRAVKATQVFTQEEDADDDAK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=518, Percent_Identity=44.980694980695, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI4504007, Length=500, Percent_Identity=45.8, Blast_Score=421, Evalue=1e-117,
Organism=Homo sapiens, GI42794763, Length=504, Percent_Identity=45.2380952380952, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI41393575, Length=517, Percent_Identity=44.294003868472, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI88196792, Length=503, Percent_Identity=32.6043737574553, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1790361, Length=502, Percent_Identity=56.7729083665339, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1790005, Length=516, Percent_Identity=22.2868217054264, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789987, Length=456, Percent_Identity=24.5614035087719, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787789, Length=487, Percent_Identity=24.2299794661191, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789168, Length=475, Percent_Identity=21.4736842105263, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=448, Percent_Identity=42.1875, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71986140, Length=515, Percent_Identity=26.0194174757282, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71986147, Length=294, Percent_Identity=30.952380952381, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=545, Percent_Identity=32.2935779816514, Blast_Score=300, Evalue=3e-82,
Organism=Drosophila melanogaster, GI17864214, Length=502, Percent_Identity=43.6254980079681, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24654573, Length=454, Percent_Identity=43.8325991189427, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24655555, Length=496, Percent_Identity=38.7096774193548, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=496, Percent_Identity=38.7096774193548, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=496, Percent_Identity=38.7096774193548, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=496, Percent_Identity=38.7096774193548, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=496, Percent_Identity=38.7096774193548, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19922058, Length=536, Percent_Identity=33.2089552238806, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24652871, Length=503, Percent_Identity=33.3996023856859, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24656367, Length=492, Percent_Identity=26.219512195122, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI21357867, Length=508, Percent_Identity=25.1968503937008, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24656372, Length=328, Percent_Identity=28.0487804878049, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI45552909, Length=280, Percent_Identity=25.3571428571429, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55997; Mature: 55866

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAGWVEHNANQIWNSVQSV
CCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAIVWQSRQTAPIAEQLKQDG
HHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCEEEHEEECCCCCCHHHHHHHHCC
HTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGELLFGTIDTWLVWKLTDGAVH
CCEEEECCCCEEEEEECCHHHHHHHHHCCCCHHHHHCCCCEEEEEECEEEEEEEECCEEE
VTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEVKSNSEIYGKTAAFHFYGGEVPI
EEECCCHHHHHEEECCCCCCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEEEEEECCCEEE
SGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGDEMQLSSNNLLTTIGYGIGGKVH
CCCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEECCCCEEEEEECCCCCEEE
YALEGSIFIAGSAIQWLRDGLKMIETSPESEQFALASTSDDEVYVVPAFTGLGAPYWDSN
EEEECCEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC
ARGSVFGLTRGTSKEDFVKATLQSIAYQVRDVIDTMQVDSGIDIQQLRVDGGAAMNNMLM
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
QFQADILGIDIARAKNLETTALGAAFLAGLAVGYWEDMDALKELNATGQLFKASMNESRK
HHHHHHHEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHH
EKLYKGWKRAVKATQVFTQEEDADDDAK
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAGWVEHNANQIWNSVQSV
CCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAIVWQSRQTAPIAEQLKQDG
HHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCEEEHEEECCCCCCHHHHHHHHCC
HTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGELLFGTIDTWLVWKLTDGAVH
CCEEEECCCCEEEEEECCHHHHHHHHHCCCCHHHHHCCCCEEEEEECEEEEEEEECCEEE
VTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEVKSNSEIYGKTAAFHFYGGEVPI
EEECCCHHHHHEEECCCCCCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEEEEEECCCEEE
SGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGDEMQLSSNNLLTTIGYGIGGKVH
CCCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEECCCCEEEEEECCCCCEEE
YALEGSIFIAGSAIQWLRDGLKMIETSPESEQFALASTSDDEVYVVPAFTGLGAPYWDSN
EEEECCEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC
ARGSVFGLTRGTSKEDFVKATLQSIAYQVRDVIDTMQVDSGIDIQQLRVDGGAAMNNMLM
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
QFQADILGIDIARAKNLETTALGAAFLAGLAVGYWEDMDALKELNATGQLFKASMNESRK
HHHHHHHEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHH
EKLYKGWKRAVKATQVFTQEEDADDDAK
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]