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The map label for this gene is gidB

Identifier: 197294687

GI number: 197294687

Start: 645351

End: 645977

Strand: Direct

Name: gidB

Synonym: PAa_0645

Alternate gene names: 197294687

Gene position: 645351-645977 (Clockwise)

Preceding gene: 197294686

Following gene: 197294688

Centisome position: 73.34

GC content: 26.32

Gene sequence:

>627_bases
GTGCTTTATCCTGATTTATTAACAAAAAAATTCAAACTTAATCCTCATCAATTGCAACAATTTGATTTATACTATCGCTT
TTTAAACCAAGAAAATAAAAAATATAATCTCACTGCTTTAATATCTTTATCAGATGTTTATTGCAAACATTTTTATGATT
CCTTGATTTTGAAAGAAATACTAGATTTTAATCAAATTCAAAATGTTTGTGATGTGGGTTCTGGAGCTGGGTTTCCTTCT
TTTCCTTTAAAAATAATTTATCCGAATTTAAAAATAACAATTGTTGAATCTTCTTTAAAAAAAATTAATTTTTTAAAACA
ATTGACAGTTGTTTTAGGTTTGAAAAACATCTGTTTTGTTCATCAAAGAGCGGAAAAACATACTTTAAGTTACGATTGTG
TTTTGGTTCGGGCTTTAGGTAGGCTGGATTTGATTTTAAAGTGGTGTCGCCTTTTAATCAAAAAAAATGGCTATTTTGTG
GCAATGAAAGGAAAAAAGTACGCAGAAGAGTTAAAAGATAGTCAACACATTATTAAAAAGATAAAAATGAATTTAATTAA
AATAAATAAACAAGAATTGCCCAATCAATTAGGGACACGAATCAATTTATTATTTCAAACTCATTAA

Upstream 100 bases:

>100_bases
TTAACTTTAATTAAACCAGAAACTTTAGGCCAGGCTTCAAGAATTTTAGGAGTCAATCAAGTAGATATTTCAATCTTGTT
AATTTATTTAGGTAAAGAAC

Downstream 100 bases:

>100_bases
AAGATAAAATAAGGCCACTAAAAATAGTGGCCTTATTTTTTTCCCGTAACTCTTAAAAGAAGGATGAAAACATTTAAAAA
AACTAAATCAATTAAATAAT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MLYPDLLTKKFKLNPHQLQQFDLYYRFLNQENKKYNLTALISLSDVYCKHFYDSLILKEILDFNQIQNVCDVGSGAGFPS
FPLKIIYPNLKITIVESSLKKINFLKQLTVVLGLKNICFVHQRAEKHTLSYDCVLVRALGRLDLILKWCRLLIKKNGYFV
AMKGKKYAEELKDSQHIIKKIKMNLIKINKQELPNQLGTRINLLFQTH

Sequences:

>Translated_208_residues
MLYPDLLTKKFKLNPHQLQQFDLYYRFLNQENKKYNLTALISLSDVYCKHFYDSLILKEILDFNQIQNVCDVGSGAGFPS
FPLKIIYPNLKITIVESSLKKINFLKQLTVVLGLKNICFVHQRAEKHTLSYDCVLVRALGRLDLILKWCRLLIKKNGYFV
AMKGKKYAEELKDSQHIIKKIKMNLIKINKQELPNQLGTRINLLFQTH
>Mature_208_residues
MLYPDLLTKKFKLNPHQLQQFDLYYRFLNQENKKYNLTALISLSDVYCKHFYDSLILKEILDFNQIQNVCDVGSGAGFPS
FPLKIIYPNLKITIVESSLKKINFLKQLTVVLGLKNICFVHQRAEKHTLSYDCVLVRALGRLDLILKWCRLLIKKNGYFV
AMKGKKYAEELKDSQHIIKKIKMNLIKINKQELPNQLGTRINLLFQTH

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=163, Percent_Identity=33.1288343558282, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_PHYAS (B1VAK6)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799228.1
- ProteinModelPortal:   B1VAK6
- SMR:   B1VAK6
- GeneID:   6799382
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG686577
- OMA:   VFIGIMF
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 24364; Mature: 24364

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPDLLTKKFKLNPHQLQQFDLYYRFLNQENKKYNLTALISLSDVYCKHFYDSLILKEI
CCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHH
LDFNQIQNVCDVGSGAGFPSFPLKIIYPNLKITIVESSLKKINFLKQLTVVLGLKNICFV
HCHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
HQRAEKHTLSYDCVLVRALGRLDLILKWCRLLIKKNGYFVAMKGKKYAEELKDSQHIIKK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHH
IKMNLIKINKQELPNQLGTRINLLFQTH
HHHHHHCCCHHHHHHHHCCEEEEEEECC
>Mature Secondary Structure
MLYPDLLTKKFKLNPHQLQQFDLYYRFLNQENKKYNLTALISLSDVYCKHFYDSLILKEI
CCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHH
LDFNQIQNVCDVGSGAGFPSFPLKIIYPNLKITIVESSLKKINFLKQLTVVLGLKNICFV
HCHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
HQRAEKHTLSYDCVLVRALGRLDLILKWCRLLIKKNGYFVAMKGKKYAEELKDSQHIIKK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHH
IKMNLIKINKQELPNQLGTRINLLFQTH
HHHHHHCCCHHHHHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA